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Reduced Mad2 expression keeps relaxed kinetochores from arresting budding yeast in mitosis

Chromosome segregation depends on the spindle checkpoint, which delays anaphase until all chromosomes have bound microtubules and have been placed under tension. The Mad1–Mad2 complex is an essential component of the checkpoint. We studied the consequences of removing one copy of MAD2 in diploid cel...

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Detalles Bibliográficos
Autores principales: Barnhart, Erin L., Dorer, Russell K., Murray, Andrew W., Schuyler, Scott C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The American Society for Cell Biology 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3135471/
https://www.ncbi.nlm.nih.gov/pubmed/21593209
http://dx.doi.org/10.1091/mbc.E09-01-0029
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author Barnhart, Erin L.
Dorer, Russell K.
Murray, Andrew W.
Schuyler, Scott C.
author_facet Barnhart, Erin L.
Dorer, Russell K.
Murray, Andrew W.
Schuyler, Scott C.
author_sort Barnhart, Erin L.
collection PubMed
description Chromosome segregation depends on the spindle checkpoint, which delays anaphase until all chromosomes have bound microtubules and have been placed under tension. The Mad1–Mad2 complex is an essential component of the checkpoint. We studied the consequences of removing one copy of MAD2 in diploid cells of the budding yeast, Saccharomyces cerevisiae. Compared to MAD2/MAD2 cells, MAD2/mad2Δ heterozygotes show increased chromosome loss and have different responses to two insults that activate the spindle checkpoint: MAD2/mad2Δ cells respond normally to antimicrotubule drugs but cannot respond to chromosomes that lack tension between sister chromatids. In MAD2/mad2Δ cells with normal sister chromatid cohesion, removing one copy of MAD1 restores the checkpoint and returns chromosome loss to wild-type levels. We conclude that cells need the normal Mad2:Mad1 ratio to respond to chromosomes that are not under tension.
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spelling pubmed-31354712011-09-30 Reduced Mad2 expression keeps relaxed kinetochores from arresting budding yeast in mitosis Barnhart, Erin L. Dorer, Russell K. Murray, Andrew W. Schuyler, Scott C. Mol Biol Cell Articles Chromosome segregation depends on the spindle checkpoint, which delays anaphase until all chromosomes have bound microtubules and have been placed under tension. The Mad1–Mad2 complex is an essential component of the checkpoint. We studied the consequences of removing one copy of MAD2 in diploid cells of the budding yeast, Saccharomyces cerevisiae. Compared to MAD2/MAD2 cells, MAD2/mad2Δ heterozygotes show increased chromosome loss and have different responses to two insults that activate the spindle checkpoint: MAD2/mad2Δ cells respond normally to antimicrotubule drugs but cannot respond to chromosomes that lack tension between sister chromatids. In MAD2/mad2Δ cells with normal sister chromatid cohesion, removing one copy of MAD1 restores the checkpoint and returns chromosome loss to wild-type levels. We conclude that cells need the normal Mad2:Mad1 ratio to respond to chromosomes that are not under tension. The American Society for Cell Biology 2011-07-15 /pmc/articles/PMC3135471/ /pubmed/21593209 http://dx.doi.org/10.1091/mbc.E09-01-0029 Text en © 2011 Barnhart et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0). “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society of Cell Biology.
spellingShingle Articles
Barnhart, Erin L.
Dorer, Russell K.
Murray, Andrew W.
Schuyler, Scott C.
Reduced Mad2 expression keeps relaxed kinetochores from arresting budding yeast in mitosis
title Reduced Mad2 expression keeps relaxed kinetochores from arresting budding yeast in mitosis
title_full Reduced Mad2 expression keeps relaxed kinetochores from arresting budding yeast in mitosis
title_fullStr Reduced Mad2 expression keeps relaxed kinetochores from arresting budding yeast in mitosis
title_full_unstemmed Reduced Mad2 expression keeps relaxed kinetochores from arresting budding yeast in mitosis
title_short Reduced Mad2 expression keeps relaxed kinetochores from arresting budding yeast in mitosis
title_sort reduced mad2 expression keeps relaxed kinetochores from arresting budding yeast in mitosis
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3135471/
https://www.ncbi.nlm.nih.gov/pubmed/21593209
http://dx.doi.org/10.1091/mbc.E09-01-0029
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