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Reduced Mad2 expression keeps relaxed kinetochores from arresting budding yeast in mitosis
Chromosome segregation depends on the spindle checkpoint, which delays anaphase until all chromosomes have bound microtubules and have been placed under tension. The Mad1–Mad2 complex is an essential component of the checkpoint. We studied the consequences of removing one copy of MAD2 in diploid cel...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The American Society for Cell Biology
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3135471/ https://www.ncbi.nlm.nih.gov/pubmed/21593209 http://dx.doi.org/10.1091/mbc.E09-01-0029 |
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author | Barnhart, Erin L. Dorer, Russell K. Murray, Andrew W. Schuyler, Scott C. |
author_facet | Barnhart, Erin L. Dorer, Russell K. Murray, Andrew W. Schuyler, Scott C. |
author_sort | Barnhart, Erin L. |
collection | PubMed |
description | Chromosome segregation depends on the spindle checkpoint, which delays anaphase until all chromosomes have bound microtubules and have been placed under tension. The Mad1–Mad2 complex is an essential component of the checkpoint. We studied the consequences of removing one copy of MAD2 in diploid cells of the budding yeast, Saccharomyces cerevisiae. Compared to MAD2/MAD2 cells, MAD2/mad2Δ heterozygotes show increased chromosome loss and have different responses to two insults that activate the spindle checkpoint: MAD2/mad2Δ cells respond normally to antimicrotubule drugs but cannot respond to chromosomes that lack tension between sister chromatids. In MAD2/mad2Δ cells with normal sister chromatid cohesion, removing one copy of MAD1 restores the checkpoint and returns chromosome loss to wild-type levels. We conclude that cells need the normal Mad2:Mad1 ratio to respond to chromosomes that are not under tension. |
format | Online Article Text |
id | pubmed-3135471 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | The American Society for Cell Biology |
record_format | MEDLINE/PubMed |
spelling | pubmed-31354712011-09-30 Reduced Mad2 expression keeps relaxed kinetochores from arresting budding yeast in mitosis Barnhart, Erin L. Dorer, Russell K. Murray, Andrew W. Schuyler, Scott C. Mol Biol Cell Articles Chromosome segregation depends on the spindle checkpoint, which delays anaphase until all chromosomes have bound microtubules and have been placed under tension. The Mad1–Mad2 complex is an essential component of the checkpoint. We studied the consequences of removing one copy of MAD2 in diploid cells of the budding yeast, Saccharomyces cerevisiae. Compared to MAD2/MAD2 cells, MAD2/mad2Δ heterozygotes show increased chromosome loss and have different responses to two insults that activate the spindle checkpoint: MAD2/mad2Δ cells respond normally to antimicrotubule drugs but cannot respond to chromosomes that lack tension between sister chromatids. In MAD2/mad2Δ cells with normal sister chromatid cohesion, removing one copy of MAD1 restores the checkpoint and returns chromosome loss to wild-type levels. We conclude that cells need the normal Mad2:Mad1 ratio to respond to chromosomes that are not under tension. The American Society for Cell Biology 2011-07-15 /pmc/articles/PMC3135471/ /pubmed/21593209 http://dx.doi.org/10.1091/mbc.E09-01-0029 Text en © 2011 Barnhart et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0). “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society of Cell Biology. |
spellingShingle | Articles Barnhart, Erin L. Dorer, Russell K. Murray, Andrew W. Schuyler, Scott C. Reduced Mad2 expression keeps relaxed kinetochores from arresting budding yeast in mitosis |
title | Reduced Mad2 expression keeps relaxed kinetochores from arresting budding yeast in mitosis |
title_full | Reduced Mad2 expression keeps relaxed kinetochores from arresting budding yeast in mitosis |
title_fullStr | Reduced Mad2 expression keeps relaxed kinetochores from arresting budding yeast in mitosis |
title_full_unstemmed | Reduced Mad2 expression keeps relaxed kinetochores from arresting budding yeast in mitosis |
title_short | Reduced Mad2 expression keeps relaxed kinetochores from arresting budding yeast in mitosis |
title_sort | reduced mad2 expression keeps relaxed kinetochores from arresting budding yeast in mitosis |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3135471/ https://www.ncbi.nlm.nih.gov/pubmed/21593209 http://dx.doi.org/10.1091/mbc.E09-01-0029 |
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