Cargando…
Analyzing Serum-Stimulated Prostate Cancer Cell Lines After Low-Fat, High-Fiber Diet and Exercise Intervention
Serum from men undergoing a low-fat, high-fiber diet and exercise intervention has previously been shown to decrease growth and increase apoptosis in serum-stimulated, androgen-dependent LNCaP cells associated with a reduction in serum IGF-I. Here we sought to determine the underlying mechanisms for...
Autores principales: | , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi Publishing Corporation
2011
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3135793/ https://www.ncbi.nlm.nih.gov/pubmed/19376839 http://dx.doi.org/10.1093/ecam/nep031 |
_version_ | 1782208144895639552 |
---|---|
author | Soliman, Sherry Aronson, William J. Barnard, R. James |
author_facet | Soliman, Sherry Aronson, William J. Barnard, R. James |
author_sort | Soliman, Sherry |
collection | PubMed |
description | Serum from men undergoing a low-fat, high-fiber diet and exercise intervention has previously been shown to decrease growth and increase apoptosis in serum-stimulated, androgen-dependent LNCaP cells associated with a reduction in serum IGF-I. Here we sought to determine the underlying mechanisms for these anticancer effects. Again, the intervention slowed growth and increased apoptosis in LNCaP cells; responses that were eliminated when IGF-I was added back to the post-intervention samples. The p53 protein content was increased and NFκB activation reduced in the post serum-stimulated LNCaP cells. Similar results were observed when the IGF-I receptor was blocked in the pre-intervention serum. In androgen-independent PC-3 cells, growth was reduced while none of the other factors were changed by the intervention. We conclude that diet and exercise intervention might help prevent clinical PCa as well as aid in the treatment of PCa during the early stages of development. |
format | Online Article Text |
id | pubmed-3135793 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-31357932011-07-22 Analyzing Serum-Stimulated Prostate Cancer Cell Lines After Low-Fat, High-Fiber Diet and Exercise Intervention Soliman, Sherry Aronson, William J. Barnard, R. James Evid Based Complement Alternat Med Original Article Serum from men undergoing a low-fat, high-fiber diet and exercise intervention has previously been shown to decrease growth and increase apoptosis in serum-stimulated, androgen-dependent LNCaP cells associated with a reduction in serum IGF-I. Here we sought to determine the underlying mechanisms for these anticancer effects. Again, the intervention slowed growth and increased apoptosis in LNCaP cells; responses that were eliminated when IGF-I was added back to the post-intervention samples. The p53 protein content was increased and NFκB activation reduced in the post serum-stimulated LNCaP cells. Similar results were observed when the IGF-I receptor was blocked in the pre-intervention serum. In androgen-independent PC-3 cells, growth was reduced while none of the other factors were changed by the intervention. We conclude that diet and exercise intervention might help prevent clinical PCa as well as aid in the treatment of PCa during the early stages of development. Hindawi Publishing Corporation 2011 2011-03-15 /pmc/articles/PMC3135793/ /pubmed/19376839 http://dx.doi.org/10.1093/ecam/nep031 Text en Copyright © 2011 Sherry Soliman et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Soliman, Sherry Aronson, William J. Barnard, R. James Analyzing Serum-Stimulated Prostate Cancer Cell Lines After Low-Fat, High-Fiber Diet and Exercise Intervention |
title | Analyzing Serum-Stimulated Prostate Cancer Cell Lines After Low-Fat, High-Fiber Diet and Exercise Intervention |
title_full | Analyzing Serum-Stimulated Prostate Cancer Cell Lines After Low-Fat, High-Fiber Diet and Exercise Intervention |
title_fullStr | Analyzing Serum-Stimulated Prostate Cancer Cell Lines After Low-Fat, High-Fiber Diet and Exercise Intervention |
title_full_unstemmed | Analyzing Serum-Stimulated Prostate Cancer Cell Lines After Low-Fat, High-Fiber Diet and Exercise Intervention |
title_short | Analyzing Serum-Stimulated Prostate Cancer Cell Lines After Low-Fat, High-Fiber Diet and Exercise Intervention |
title_sort | analyzing serum-stimulated prostate cancer cell lines after low-fat, high-fiber diet and exercise intervention |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3135793/ https://www.ncbi.nlm.nih.gov/pubmed/19376839 http://dx.doi.org/10.1093/ecam/nep031 |
work_keys_str_mv | AT solimansherry analyzingserumstimulatedprostatecancercelllinesafterlowfathighfiberdietandexerciseintervention AT aronsonwilliamj analyzingserumstimulatedprostatecancercelllinesafterlowfathighfiberdietandexerciseintervention AT barnardrjames analyzingserumstimulatedprostatecancercelllinesafterlowfathighfiberdietandexerciseintervention |