Autoassembly Protein Arrays for Analyzing Antibody Cross-Reactivity

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[Image: see text] We report an autoassembly protein array capable of rapidly screening for aberrant antibody−antigen binding events. Our technique combines magnetic nanoparticle technology with proximity-based, magnetically responsive nanosensors for rapid (under 15 min) and high-density screening o...

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Detalles Bibliográficos
Autores principales: Gaster, Richard S., Hall, Drew A., Wang, Shan X.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2010
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3136110/
https://www.ncbi.nlm.nih.gov/pubmed/20804215
http://dx.doi.org/10.1021/nl1026056
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author Gaster, Richard S.
Hall, Drew A.
Wang, Shan X.
author_facet Gaster, Richard S.
Hall, Drew A.
Wang, Shan X.
author_sort Gaster, Richard S.
collection PubMed
description [Image: see text] We report an autoassembly protein array capable of rapidly screening for aberrant antibody−antigen binding events. Our technique combines magnetic nanoparticle technology with proximity-based, magnetically responsive nanosensors for rapid (under 15 min) and high-density screening of antibody cross-reactivity at sensitivities down to 50 fM in a homogeneous assay. This method will enable the identification of the precise cause of aberrant or cross-reactive binding events in an easy-to-use, rapid, and high-throughput manner.
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spelling pubmed-31361102011-07-14 Autoassembly Protein Arrays for Analyzing Antibody Cross-Reactivity Gaster, Richard S. Hall, Drew A. Wang, Shan X. Nano Lett [Image: see text] We report an autoassembly protein array capable of rapidly screening for aberrant antibody−antigen binding events. Our technique combines magnetic nanoparticle technology with proximity-based, magnetically responsive nanosensors for rapid (under 15 min) and high-density screening of antibody cross-reactivity at sensitivities down to 50 fM in a homogeneous assay. This method will enable the identification of the precise cause of aberrant or cross-reactive binding events in an easy-to-use, rapid, and high-throughput manner. American Chemical Society 2010-08-30 2011-07-13 /pmc/articles/PMC3136110/ /pubmed/20804215 http://dx.doi.org/10.1021/nl1026056 Text en Copyright © 2010 American Chemical Society http://pubs.acs.org This is an open-access article distributed under the ACS AuthorChoice Terms & Conditions. Any use of this article, must conform to the terms of that license which are available at http://pubs.acs.org.
spellingShingle Gaster, Richard S.
Hall, Drew A.
Wang, Shan X.
Autoassembly Protein Arrays for Analyzing Antibody Cross-Reactivity
title Autoassembly Protein Arrays for Analyzing Antibody Cross-Reactivity
title_full Autoassembly Protein Arrays for Analyzing Antibody Cross-Reactivity
title_fullStr Autoassembly Protein Arrays for Analyzing Antibody Cross-Reactivity
title_full_unstemmed Autoassembly Protein Arrays for Analyzing Antibody Cross-Reactivity
title_short Autoassembly Protein Arrays for Analyzing Antibody Cross-Reactivity
title_sort autoassembly protein arrays for analyzing antibody cross-reactivity
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3136110/
https://www.ncbi.nlm.nih.gov/pubmed/20804215
http://dx.doi.org/10.1021/nl1026056
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AT wangshanx autoassemblyproteinarraysforanalyzingantibodycrossreactivity

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