On-Chip DNA Methylation Analysis Using Osmium Complexation

The development of a reaction for detecting the presence/absence of one methyl group in a long DNA strand is a chemically and biologically challenging research subject. A newly designed chemical assay on a chip for the typing of DNA methylation has been developed. A methylation-detection probe fixed...

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Detalles Bibliográficos
Autores principales: Sugizaki, Kaori, Umemoto, Tadashi, Okamoto, Akimitsu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: SAGE-Hindawi Access to Research 2011
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3136155/
https://www.ncbi.nlm.nih.gov/pubmed/21772994
http://dx.doi.org/10.4061/2011/480570
Descripción
Sumario:The development of a reaction for detecting the presence/absence of one methyl group in a long DNA strand is a chemically and biologically challenging research subject. A newly designed chemical assay on a chip for the typing of DNA methylation has been developed. A methylation-detection probe fixed at the bottom of microwells was crosslinked with methylated DNA mediated by osmium complexation and contributes to selective amplification of methylated DNA.

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