On-Chip DNA Methylation Analysis Using Osmium Complexation

The development of a reaction for detecting the presence/absence of one methyl group in a long DNA strand is a chemically and biologically challenging research subject. A newly designed chemical assay on a chip for the typing of DNA methylation has been developed. A methylation-detection probe fixed...

Descripción completa

Detalles Bibliográficos
Autores principales: Sugizaki, Kaori, Umemoto, Tadashi, Okamoto, Akimitsu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: SAGE-Hindawi Access to Research 2011
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3136155/
https://www.ncbi.nlm.nih.gov/pubmed/21772994
http://dx.doi.org/10.4061/2011/480570
_version_ 1782208178879987712
author Sugizaki, Kaori
Umemoto, Tadashi
Okamoto, Akimitsu
author_facet Sugizaki, Kaori
Umemoto, Tadashi
Okamoto, Akimitsu
author_sort Sugizaki, Kaori
collection PubMed
description The development of a reaction for detecting the presence/absence of one methyl group in a long DNA strand is a chemically and biologically challenging research subject. A newly designed chemical assay on a chip for the typing of DNA methylation has been developed. A methylation-detection probe fixed at the bottom of microwells was crosslinked with methylated DNA mediated by osmium complexation and contributes to selective amplification of methylated DNA.
format Online
Article
Text
id pubmed-3136155
institution National Center for Biotechnology Information
language English
publishDate 2011
publisher SAGE-Hindawi Access to Research
record_format MEDLINE/PubMed
spelling pubmed-31361552011-07-19 On-Chip DNA Methylation Analysis Using Osmium Complexation Sugizaki, Kaori Umemoto, Tadashi Okamoto, Akimitsu J Nucleic Acids Research Article The development of a reaction for detecting the presence/absence of one methyl group in a long DNA strand is a chemically and biologically challenging research subject. A newly designed chemical assay on a chip for the typing of DNA methylation has been developed. A methylation-detection probe fixed at the bottom of microwells was crosslinked with methylated DNA mediated by osmium complexation and contributes to selective amplification of methylated DNA. SAGE-Hindawi Access to Research 2011-05-15 /pmc/articles/PMC3136155/ /pubmed/21772994 http://dx.doi.org/10.4061/2011/480570 Text en Copyright © 2011 Kaori Sugizaki et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Sugizaki, Kaori
Umemoto, Tadashi
Okamoto, Akimitsu
On-Chip DNA Methylation Analysis Using Osmium Complexation
title On-Chip DNA Methylation Analysis Using Osmium Complexation
title_full On-Chip DNA Methylation Analysis Using Osmium Complexation
title_fullStr On-Chip DNA Methylation Analysis Using Osmium Complexation
title_full_unstemmed On-Chip DNA Methylation Analysis Using Osmium Complexation
title_short On-Chip DNA Methylation Analysis Using Osmium Complexation
title_sort on-chip dna methylation analysis using osmium complexation
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3136155/
https://www.ncbi.nlm.nih.gov/pubmed/21772994
http://dx.doi.org/10.4061/2011/480570
work_keys_str_mv AT sugizakikaori onchipdnamethylationanalysisusingosmiumcomplexation
AT umemototadashi onchipdnamethylationanalysisusingosmiumcomplexation
AT okamotoakimitsu onchipdnamethylationanalysisusingosmiumcomplexation

Ejemplares similares