Cargando…

Cross-Species Transmission of a Novel Adenovirus Associated with a Fulminant Pneumonia Outbreak in a New World Monkey Colony

Adenoviruses are DNA viruses that naturally infect many vertebrates, including humans and monkeys, and cause a wide range of clinical illnesses in humans. Infection from individual strains has conventionally been thought to be species-specific. Here we applied the Virochip, a pan-viral microarray, t...

Descripción completa

Detalles Bibliográficos
Autores principales: Chen, Eunice C., Yagi, Shigeo, Kelly, Kristi R., Mendoza, Sally P., Maninger, Nicole, Rosenthal, Ann, Spinner, Abigail, Bales, Karen L., Schnurr, David P., Lerche, Nicholas W., Chiu, Charles Y.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3136464/
https://www.ncbi.nlm.nih.gov/pubmed/21779173
http://dx.doi.org/10.1371/journal.ppat.1002155
_version_ 1782208209431298048
author Chen, Eunice C.
Yagi, Shigeo
Kelly, Kristi R.
Mendoza, Sally P.
Maninger, Nicole
Rosenthal, Ann
Spinner, Abigail
Bales, Karen L.
Schnurr, David P.
Lerche, Nicholas W.
Chiu, Charles Y.
author_facet Chen, Eunice C.
Yagi, Shigeo
Kelly, Kristi R.
Mendoza, Sally P.
Maninger, Nicole
Rosenthal, Ann
Spinner, Abigail
Bales, Karen L.
Schnurr, David P.
Lerche, Nicholas W.
Chiu, Charles Y.
author_sort Chen, Eunice C.
collection PubMed
description Adenoviruses are DNA viruses that naturally infect many vertebrates, including humans and monkeys, and cause a wide range of clinical illnesses in humans. Infection from individual strains has conventionally been thought to be species-specific. Here we applied the Virochip, a pan-viral microarray, to identify a novel adenovirus (TMAdV, titi monkey adenovirus) as the cause of a deadly outbreak in a closed colony of New World monkeys (titi monkeys; Callicebus cupreus) at the California National Primate Research Center (CNPRC). Among 65 titi monkeys housed in a building, 23 (34%) developed upper respiratory symptoms that progressed to fulminant pneumonia and hepatitis, and 19 of 23 monkeys, or 83% of those infected, died or were humanely euthanized. Whole-genome sequencing of TMAdV revealed that this adenovirus is a new species and highly divergent, sharing <57% pairwise nucleotide identity with other adenoviruses. Cultivation of TMAdV was successful in a human A549 lung adenocarcinoma cell line, but not in primary or established monkey kidney cells. At the onset of the outbreak, the researcher in closest contact with the monkeys developed an acute respiratory illness, with symptoms persisting for 4 weeks, and had a convalescent serum sample seropositive for TMAdV. A clinically ill family member, despite having no contact with the CNPRC, also tested positive, and screening of a set of 81 random adult blood donors from the Western United States detected TMAdV-specific neutralizing antibodies in 2 individuals (2/81, or 2.5%). These findings raise the possibility of zoonotic infection by TMAdV and human-to-human transmission of the virus in the population. Given the unusually high case fatality rate from the outbreak (83%), it is unlikely that titi monkeys are the native host species for TMAdV, and the natural reservoir of the virus is still unknown. The discovery of TMAdV, a novel adenovirus with the capacity to infect both monkeys and humans, suggests that adenoviruses should be monitored closely as potential causes of cross-species outbreaks.
format Online
Article
Text
id pubmed-3136464
institution National Center for Biotechnology Information
language English
publishDate 2011
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-31364642011-07-21 Cross-Species Transmission of a Novel Adenovirus Associated with a Fulminant Pneumonia Outbreak in a New World Monkey Colony Chen, Eunice C. Yagi, Shigeo Kelly, Kristi R. Mendoza, Sally P. Maninger, Nicole Rosenthal, Ann Spinner, Abigail Bales, Karen L. Schnurr, David P. Lerche, Nicholas W. Chiu, Charles Y. PLoS Pathog Research Article Adenoviruses are DNA viruses that naturally infect many vertebrates, including humans and monkeys, and cause a wide range of clinical illnesses in humans. Infection from individual strains has conventionally been thought to be species-specific. Here we applied the Virochip, a pan-viral microarray, to identify a novel adenovirus (TMAdV, titi monkey adenovirus) as the cause of a deadly outbreak in a closed colony of New World monkeys (titi monkeys; Callicebus cupreus) at the California National Primate Research Center (CNPRC). Among 65 titi monkeys housed in a building, 23 (34%) developed upper respiratory symptoms that progressed to fulminant pneumonia and hepatitis, and 19 of 23 monkeys, or 83% of those infected, died or were humanely euthanized. Whole-genome sequencing of TMAdV revealed that this adenovirus is a new species and highly divergent, sharing <57% pairwise nucleotide identity with other adenoviruses. Cultivation of TMAdV was successful in a human A549 lung adenocarcinoma cell line, but not in primary or established monkey kidney cells. At the onset of the outbreak, the researcher in closest contact with the monkeys developed an acute respiratory illness, with symptoms persisting for 4 weeks, and had a convalescent serum sample seropositive for TMAdV. A clinically ill family member, despite having no contact with the CNPRC, also tested positive, and screening of a set of 81 random adult blood donors from the Western United States detected TMAdV-specific neutralizing antibodies in 2 individuals (2/81, or 2.5%). These findings raise the possibility of zoonotic infection by TMAdV and human-to-human transmission of the virus in the population. Given the unusually high case fatality rate from the outbreak (83%), it is unlikely that titi monkeys are the native host species for TMAdV, and the natural reservoir of the virus is still unknown. The discovery of TMAdV, a novel adenovirus with the capacity to infect both monkeys and humans, suggests that adenoviruses should be monitored closely as potential causes of cross-species outbreaks. Public Library of Science 2011-07-14 /pmc/articles/PMC3136464/ /pubmed/21779173 http://dx.doi.org/10.1371/journal.ppat.1002155 Text en Chen et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Chen, Eunice C.
Yagi, Shigeo
Kelly, Kristi R.
Mendoza, Sally P.
Maninger, Nicole
Rosenthal, Ann
Spinner, Abigail
Bales, Karen L.
Schnurr, David P.
Lerche, Nicholas W.
Chiu, Charles Y.
Cross-Species Transmission of a Novel Adenovirus Associated with a Fulminant Pneumonia Outbreak in a New World Monkey Colony
title Cross-Species Transmission of a Novel Adenovirus Associated with a Fulminant Pneumonia Outbreak in a New World Monkey Colony
title_full Cross-Species Transmission of a Novel Adenovirus Associated with a Fulminant Pneumonia Outbreak in a New World Monkey Colony
title_fullStr Cross-Species Transmission of a Novel Adenovirus Associated with a Fulminant Pneumonia Outbreak in a New World Monkey Colony
title_full_unstemmed Cross-Species Transmission of a Novel Adenovirus Associated with a Fulminant Pneumonia Outbreak in a New World Monkey Colony
title_short Cross-Species Transmission of a Novel Adenovirus Associated with a Fulminant Pneumonia Outbreak in a New World Monkey Colony
title_sort cross-species transmission of a novel adenovirus associated with a fulminant pneumonia outbreak in a new world monkey colony
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3136464/
https://www.ncbi.nlm.nih.gov/pubmed/21779173
http://dx.doi.org/10.1371/journal.ppat.1002155
work_keys_str_mv AT cheneunicec crossspeciestransmissionofanoveladenovirusassociatedwithafulminantpneumoniaoutbreakinanewworldmonkeycolony
AT yagishigeo crossspeciestransmissionofanoveladenovirusassociatedwithafulminantpneumoniaoutbreakinanewworldmonkeycolony
AT kellykristir crossspeciestransmissionofanoveladenovirusassociatedwithafulminantpneumoniaoutbreakinanewworldmonkeycolony
AT mendozasallyp crossspeciestransmissionofanoveladenovirusassociatedwithafulminantpneumoniaoutbreakinanewworldmonkeycolony
AT maningernicole crossspeciestransmissionofanoveladenovirusassociatedwithafulminantpneumoniaoutbreakinanewworldmonkeycolony
AT rosenthalann crossspeciestransmissionofanoveladenovirusassociatedwithafulminantpneumoniaoutbreakinanewworldmonkeycolony
AT spinnerabigail crossspeciestransmissionofanoveladenovirusassociatedwithafulminantpneumoniaoutbreakinanewworldmonkeycolony
AT baleskarenl crossspeciestransmissionofanoveladenovirusassociatedwithafulminantpneumoniaoutbreakinanewworldmonkeycolony
AT schnurrdavidp crossspeciestransmissionofanoveladenovirusassociatedwithafulminantpneumoniaoutbreakinanewworldmonkeycolony
AT lerchenicholasw crossspeciestransmissionofanoveladenovirusassociatedwithafulminantpneumoniaoutbreakinanewworldmonkeycolony
AT chiucharlesy crossspeciestransmissionofanoveladenovirusassociatedwithafulminantpneumoniaoutbreakinanewworldmonkeycolony