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Detection of Periodontal Markers in Chronic Periodontitis

The aim was to compare the detection frequency of periodontopathogens by using the Pado Test 4.5 and checkerboard DNA-DNA hybridization technique in chronic periodontitis patients. Thirty patients with chronic periodontitis were tested cross-sectionally with DNA/RNA oligogenomic probe method (IAI Pa...

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Detalles Bibliográficos
Autores principales: Leonhardt, Åsa, Carlén, Anette, Bengtsson, Lisbeth, Dahlén, Gunnar
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Bentham Open 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3136968/
https://www.ncbi.nlm.nih.gov/pubmed/21769304
http://dx.doi.org/10.2174/1874210601105010110
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author Leonhardt, Åsa
Carlén, Anette
Bengtsson, Lisbeth
Dahlén, Gunnar
author_facet Leonhardt, Åsa
Carlén, Anette
Bengtsson, Lisbeth
Dahlén, Gunnar
author_sort Leonhardt, Åsa
collection PubMed
description The aim was to compare the detection frequency of periodontopathogens by using the Pado Test 4.5 and checkerboard DNA-DNA hybridization technique in chronic periodontitis patients. Thirty patients with chronic periodontitis were tested cross-sectionally with DNA/RNA oligogenomic probe method (IAI Pado Test 4.5) and DNA/DNA whole genomic probe (checkerboard) method. Samples were taken by two paper points at the deepest site in each of the four quadrants and pooled into one sample for each of the two methods. The samples were sent to the two laboratories (IAI, Zuchwil, Switzerland, and Oral Microbiology Laboratory, University of Gothenburg, Sweden) and were analyzed in a routine setting for the presence and amount of Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, Tannerella forsythia and Treponema denticola. While Pado Test 4.5 detected the four periodontal pathogens in 11 (36.7%) of the patients, the checkerboard method showed presence in all patients (100%) using the lower score (Score 1 corresponding to 10(4) bacterial cells) and 16 (53.3%) using a higher treshold (score 3 corresponding to between >10(5) and 10(6) cells). The results of the present study showed low agreement for a positive microbiological outcome using the two diagnostic methods. It was also concluded that microbiological analysis in practice should include a larger number of bacterial species to better serve as markers for a diseased associated flora in chronic periodontitis cases.
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spelling pubmed-31369682011-07-18 Detection of Periodontal Markers in Chronic Periodontitis Leonhardt, Åsa Carlén, Anette Bengtsson, Lisbeth Dahlén, Gunnar Open Dent J Article The aim was to compare the detection frequency of periodontopathogens by using the Pado Test 4.5 and checkerboard DNA-DNA hybridization technique in chronic periodontitis patients. Thirty patients with chronic periodontitis were tested cross-sectionally with DNA/RNA oligogenomic probe method (IAI Pado Test 4.5) and DNA/DNA whole genomic probe (checkerboard) method. Samples were taken by two paper points at the deepest site in each of the four quadrants and pooled into one sample for each of the two methods. The samples were sent to the two laboratories (IAI, Zuchwil, Switzerland, and Oral Microbiology Laboratory, University of Gothenburg, Sweden) and were analyzed in a routine setting for the presence and amount of Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, Tannerella forsythia and Treponema denticola. While Pado Test 4.5 detected the four periodontal pathogens in 11 (36.7%) of the patients, the checkerboard method showed presence in all patients (100%) using the lower score (Score 1 corresponding to 10(4) bacterial cells) and 16 (53.3%) using a higher treshold (score 3 corresponding to between >10(5) and 10(6) cells). The results of the present study showed low agreement for a positive microbiological outcome using the two diagnostic methods. It was also concluded that microbiological analysis in practice should include a larger number of bacterial species to better serve as markers for a diseased associated flora in chronic periodontitis cases. Bentham Open 2011-07-07 /pmc/articles/PMC3136968/ /pubmed/21769304 http://dx.doi.org/10.2174/1874210601105010110 Text en © Leonhardt et al.; Licensee Bentham Open. http://creativecommons.org/licenses/by-nc/3.0/ This is an open access article licensed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted, non-commercial use, distribution and reproduction in any medium, provided the work is properly cited.
spellingShingle Article
Leonhardt, Åsa
Carlén, Anette
Bengtsson, Lisbeth
Dahlén, Gunnar
Detection of Periodontal Markers in Chronic Periodontitis
title Detection of Periodontal Markers in Chronic Periodontitis
title_full Detection of Periodontal Markers in Chronic Periodontitis
title_fullStr Detection of Periodontal Markers in Chronic Periodontitis
title_full_unstemmed Detection of Periodontal Markers in Chronic Periodontitis
title_short Detection of Periodontal Markers in Chronic Periodontitis
title_sort detection of periodontal markers in chronic periodontitis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3136968/
https://www.ncbi.nlm.nih.gov/pubmed/21769304
http://dx.doi.org/10.2174/1874210601105010110
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