Cargando…
Cerebrolysin Attenuates Heat Shock Protein (HSP 72 KD) Expression in the Rat Spinal Cord Following Morphine Dependence and Withdrawal: Possible New Therapy for Pain Management
The possibility that pain perception and processing in the CNS results in cellular stress and may influence heat shock protein (HSP) expression was examined in a rat model of morphine dependence and withdrawal. Since activation of pain pathways result in exhaustion of growth factors, we examined the...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Bentham Science Publishers Ltd
2011
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3137188/ https://www.ncbi.nlm.nih.gov/pubmed/21886595 http://dx.doi.org/10.2174/157015911795017100 |
_version_ | 1782208269500022784 |
---|---|
author | Sharma, Hari S Ali, Syed F Patnaik, Ranjana Zimmermann-Meinzingen, Sibilla Sharma, Aruna Muresanu, Dafin F |
author_facet | Sharma, Hari S Ali, Syed F Patnaik, Ranjana Zimmermann-Meinzingen, Sibilla Sharma, Aruna Muresanu, Dafin F |
author_sort | Sharma, Hari S |
collection | PubMed |
description | The possibility that pain perception and processing in the CNS results in cellular stress and may influence heat shock protein (HSP) expression was examined in a rat model of morphine dependence and withdrawal. Since activation of pain pathways result in exhaustion of growth factors, we examined the influence of cerebrolysin, a mixture of potent growth factors (BDNF, GDNF, NGF, CNTF etc,) on morphine induced HSP expression. Rats were administered morphine (10 mg/kg, s.c. /day) for 12 days and the spontaneous withdrawal symptoms were developed by cessation of the drug administration on day 13(th) that were prominent on day 14(th) and continued up to day 15(th) (24 to 72 h periods). In a separate group of rats, cerebrolysin was infused intravenously (5 ml/kg) once daily from day one until day 15(th). In these animals, morphine dependence and withdrawal along with HSP immunoreactivity was examined using standard protocol. In untreated group mild HSP immunoreaction was observed during morphine tolerance, whereas massive upregulation of HSP was seen in CNS during withdrawal phase that correlated well with the withdrawal symptoms and neuronal damage. Pretreatment with cerebrolysin did not affect morphine tolerance but reduced the HSP expression during this phase. Furthermore, cerebrolysin reduced the withdrawal symptoms on day 14(th) to 15(th). Taken together these observations suggest that cellular stress plays an important role in morphine induced pain pathology and exogenous supplement of growth factors, i.e. cerebrolysin attenuates HSP expression in the CNS and induce neuroprotection. This indicates a new therapeutic role of cerebrolysin in the pathophysiology of drugs of abuse, not reported earlier. |
format | Online Article Text |
id | pubmed-3137188 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | Bentham Science Publishers Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-31371882011-09-01 Cerebrolysin Attenuates Heat Shock Protein (HSP 72 KD) Expression in the Rat Spinal Cord Following Morphine Dependence and Withdrawal: Possible New Therapy for Pain Management Sharma, Hari S Ali, Syed F Patnaik, Ranjana Zimmermann-Meinzingen, Sibilla Sharma, Aruna Muresanu, Dafin F Curr Neuropharmacol Article The possibility that pain perception and processing in the CNS results in cellular stress and may influence heat shock protein (HSP) expression was examined in a rat model of morphine dependence and withdrawal. Since activation of pain pathways result in exhaustion of growth factors, we examined the influence of cerebrolysin, a mixture of potent growth factors (BDNF, GDNF, NGF, CNTF etc,) on morphine induced HSP expression. Rats were administered morphine (10 mg/kg, s.c. /day) for 12 days and the spontaneous withdrawal symptoms were developed by cessation of the drug administration on day 13(th) that were prominent on day 14(th) and continued up to day 15(th) (24 to 72 h periods). In a separate group of rats, cerebrolysin was infused intravenously (5 ml/kg) once daily from day one until day 15(th). In these animals, morphine dependence and withdrawal along with HSP immunoreactivity was examined using standard protocol. In untreated group mild HSP immunoreaction was observed during morphine tolerance, whereas massive upregulation of HSP was seen in CNS during withdrawal phase that correlated well with the withdrawal symptoms and neuronal damage. Pretreatment with cerebrolysin did not affect morphine tolerance but reduced the HSP expression during this phase. Furthermore, cerebrolysin reduced the withdrawal symptoms on day 14(th) to 15(th). Taken together these observations suggest that cellular stress plays an important role in morphine induced pain pathology and exogenous supplement of growth factors, i.e. cerebrolysin attenuates HSP expression in the CNS and induce neuroprotection. This indicates a new therapeutic role of cerebrolysin in the pathophysiology of drugs of abuse, not reported earlier. Bentham Science Publishers Ltd 2011-03 /pmc/articles/PMC3137188/ /pubmed/21886595 http://dx.doi.org/10.2174/157015911795017100 Text en ©2011 Bentham Science Publishers Ltd. http://creativecommons.org/licenses/by/2.5/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.5/), which permits unrestrictive use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Article Sharma, Hari S Ali, Syed F Patnaik, Ranjana Zimmermann-Meinzingen, Sibilla Sharma, Aruna Muresanu, Dafin F Cerebrolysin Attenuates Heat Shock Protein (HSP 72 KD) Expression in the Rat Spinal Cord Following Morphine Dependence and Withdrawal: Possible New Therapy for Pain Management |
title | Cerebrolysin Attenuates Heat Shock Protein (HSP 72 KD) Expression in the Rat Spinal Cord Following Morphine Dependence and Withdrawal: Possible New Therapy for Pain Management |
title_full | Cerebrolysin Attenuates Heat Shock Protein (HSP 72 KD) Expression in the Rat Spinal Cord Following Morphine Dependence and Withdrawal: Possible New Therapy for Pain Management |
title_fullStr | Cerebrolysin Attenuates Heat Shock Protein (HSP 72 KD) Expression in the Rat Spinal Cord Following Morphine Dependence and Withdrawal: Possible New Therapy for Pain Management |
title_full_unstemmed | Cerebrolysin Attenuates Heat Shock Protein (HSP 72 KD) Expression in the Rat Spinal Cord Following Morphine Dependence and Withdrawal: Possible New Therapy for Pain Management |
title_short | Cerebrolysin Attenuates Heat Shock Protein (HSP 72 KD) Expression in the Rat Spinal Cord Following Morphine Dependence and Withdrawal: Possible New Therapy for Pain Management |
title_sort | cerebrolysin attenuates heat shock protein (hsp 72 kd) expression in the rat spinal cord following morphine dependence and withdrawal: possible new therapy for pain management |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3137188/ https://www.ncbi.nlm.nih.gov/pubmed/21886595 http://dx.doi.org/10.2174/157015911795017100 |
work_keys_str_mv | AT sharmaharis cerebrolysinattenuatesheatshockproteinhsp72kdexpressionintheratspinalcordfollowingmorphinedependenceandwithdrawalpossiblenewtherapyforpainmanagement AT alisyedf cerebrolysinattenuatesheatshockproteinhsp72kdexpressionintheratspinalcordfollowingmorphinedependenceandwithdrawalpossiblenewtherapyforpainmanagement AT patnaikranjana cerebrolysinattenuatesheatshockproteinhsp72kdexpressionintheratspinalcordfollowingmorphinedependenceandwithdrawalpossiblenewtherapyforpainmanagement AT zimmermannmeinzingensibilla cerebrolysinattenuatesheatshockproteinhsp72kdexpressionintheratspinalcordfollowingmorphinedependenceandwithdrawalpossiblenewtherapyforpainmanagement AT sharmaaruna cerebrolysinattenuatesheatshockproteinhsp72kdexpressionintheratspinalcordfollowingmorphinedependenceandwithdrawalpossiblenewtherapyforpainmanagement AT muresanudafinf cerebrolysinattenuatesheatshockproteinhsp72kdexpressionintheratspinalcordfollowingmorphinedependenceandwithdrawalpossiblenewtherapyforpainmanagement |