Pathogen detection using short-RNA deep sequencing subtraction and assembly

Motivation: Early and accurate detection of human pathogen infection is critical for treatment and therapeutics. Here we describe pathogen identification using short RNA subtraction and assembly (SRSA), a detection method that overcomes the requirement of prior knowledge and culturing of pathogens,...

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Detalles Bibliográficos
Autores principales: Isakov, Ofer, Modai, Shira, Shomron, Noam
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2011
Materias:
Discovery Note
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3137223/
https://www.ncbi.nlm.nih.gov/pubmed/21666269
http://dx.doi.org/10.1093/bioinformatics/btr349
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author Isakov, Ofer
Modai, Shira
Shomron, Noam
author_facet Isakov, Ofer
Modai, Shira
Shomron, Noam
author_sort Isakov, Ofer
collection PubMed
description Motivation: Early and accurate detection of human pathogen infection is critical for treatment and therapeutics. Here we describe pathogen identification using short RNA subtraction and assembly (SRSA), a detection method that overcomes the requirement of prior knowledge and culturing of pathogens, by using degraded small RNA and deep sequencing technology. We prove our approach's efficiency through identification of a combined viral and bacterial infection in human cells. Contact: nshomron@post.tau.ac.il
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spelling pubmed-31372232011-07-15 Pathogen detection using short-RNA deep sequencing subtraction and assembly Isakov, Ofer Modai, Shira Shomron, Noam Bioinformatics Discovery Note Motivation: Early and accurate detection of human pathogen infection is critical for treatment and therapeutics. Here we describe pathogen identification using short RNA subtraction and assembly (SRSA), a detection method that overcomes the requirement of prior knowledge and culturing of pathogens, by using degraded small RNA and deep sequencing technology. We prove our approach's efficiency through identification of a combined viral and bacterial infection in human cells. Contact: nshomron@post.tau.ac.il Oxford University Press 2011-08-01 2011-06-11 /pmc/articles/PMC3137223/ /pubmed/21666269 http://dx.doi.org/10.1093/bioinformatics/btr349 Text en © The Author(s) 2011. Published by Oxford University Press. http://creativecommons.org/licenses/by-nc/2.5 This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Discovery Note
Isakov, Ofer
Modai, Shira
Shomron, Noam
Pathogen detection using short-RNA deep sequencing subtraction and assembly
title Pathogen detection using short-RNA deep sequencing subtraction and assembly
title_full Pathogen detection using short-RNA deep sequencing subtraction and assembly
title_fullStr Pathogen detection using short-RNA deep sequencing subtraction and assembly
title_full_unstemmed Pathogen detection using short-RNA deep sequencing subtraction and assembly
title_short Pathogen detection using short-RNA deep sequencing subtraction and assembly
title_sort pathogen detection using short-rna deep sequencing subtraction and assembly
topic Discovery Note
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3137223/
https://www.ncbi.nlm.nih.gov/pubmed/21666269
http://dx.doi.org/10.1093/bioinformatics/btr349
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