Sequencing technology does not eliminate biological variability

RNA sequencing has generated much excitement for the advantages offered over microarrays. This excitement has led to a barrage of publications discounting the importance of biological variability; as microarray publications did in the 1990s. By comparing microarray and sequencing data, we demonstrat...

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Detalles Bibliográficos
Autores principales: Hansen, Kasper D., Wu, Zhijin, Irizarry, Rafael A., Leek, Jeffrey T.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2011
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3137276/
https://www.ncbi.nlm.nih.gov/pubmed/21747377
http://dx.doi.org/10.1038/nbt.1910
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author Hansen, Kasper D.
Wu, Zhijin
Irizarry, Rafael A.
Leek, Jeffrey T.
author_facet Hansen, Kasper D.
Wu, Zhijin
Irizarry, Rafael A.
Leek, Jeffrey T.
author_sort Hansen, Kasper D.
collection PubMed
description RNA sequencing has generated much excitement for the advantages offered over microarrays. This excitement has led to a barrage of publications discounting the importance of biological variability; as microarray publications did in the 1990s. By comparing microarray and sequencing data, we demonstrate that expression measurements exhibit biological variability across individuals irrespective of measurement technology. Our analysis suggests RNA-sequencing experiments designed to estimate biological variability are more likely to produce reproducible results.
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spelling pubmed-31372762012-01-11 Sequencing technology does not eliminate biological variability Hansen, Kasper D. Wu, Zhijin Irizarry, Rafael A. Leek, Jeffrey T. Nat Biotechnol Article RNA sequencing has generated much excitement for the advantages offered over microarrays. This excitement has led to a barrage of publications discounting the importance of biological variability; as microarray publications did in the 1990s. By comparing microarray and sequencing data, we demonstrate that expression measurements exhibit biological variability across individuals irrespective of measurement technology. Our analysis suggests RNA-sequencing experiments designed to estimate biological variability are more likely to produce reproducible results. 2011-07-11 /pmc/articles/PMC3137276/ /pubmed/21747377 http://dx.doi.org/10.1038/nbt.1910 Text en Users may view, print, copy, download and text and data- mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use: http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Hansen, Kasper D.
Wu, Zhijin
Irizarry, Rafael A.
Leek, Jeffrey T.
Sequencing technology does not eliminate biological variability
title Sequencing technology does not eliminate biological variability
title_full Sequencing technology does not eliminate biological variability
title_fullStr Sequencing technology does not eliminate biological variability
title_full_unstemmed Sequencing technology does not eliminate biological variability
title_short Sequencing technology does not eliminate biological variability
title_sort sequencing technology does not eliminate biological variability
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3137276/
https://www.ncbi.nlm.nih.gov/pubmed/21747377
http://dx.doi.org/10.1038/nbt.1910
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