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Metabolic Versatility and Antibacterial Metabolite Biosynthesis Are Distinguishing Genomic Features of the Fire Blight Antagonist Pantoea vagans C9-1

BACKGROUND: Pantoea vagans is a commercialized biological control agent used against the pome fruit bacterial disease fire blight, caused by Erwinia amylovora. Compared to other biocontrol agents, relatively little is currently known regarding Pantoea genetics. Better understanding of antagonist mec...

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Autores principales: Smits, Theo H. M., Rezzonico, Fabio, Kamber, Tim, Blom, Jochen, Goesmann, Alexander, Ishimaru, Carol A., Frey, Jürg E., Stockwell, Virginia O., Duffy, Brion
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3137637/
https://www.ncbi.nlm.nih.gov/pubmed/21789243
http://dx.doi.org/10.1371/journal.pone.0022247
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author Smits, Theo H. M.
Rezzonico, Fabio
Kamber, Tim
Blom, Jochen
Goesmann, Alexander
Ishimaru, Carol A.
Frey, Jürg E.
Stockwell, Virginia O.
Duffy, Brion
author_facet Smits, Theo H. M.
Rezzonico, Fabio
Kamber, Tim
Blom, Jochen
Goesmann, Alexander
Ishimaru, Carol A.
Frey, Jürg E.
Stockwell, Virginia O.
Duffy, Brion
author_sort Smits, Theo H. M.
collection PubMed
description BACKGROUND: Pantoea vagans is a commercialized biological control agent used against the pome fruit bacterial disease fire blight, caused by Erwinia amylovora. Compared to other biocontrol agents, relatively little is currently known regarding Pantoea genetics. Better understanding of antagonist mechanisms of action and ecological fitness is critical to improving efficacy. PRINCIPAL FINDINGS: Genome analysis indicated two major factors contribute to biocontrol activity: competition for limiting substrates and antibacterial metabolite production. Pathways for utilization of a broad diversity of sugars and acquisition of iron were identified. Metabolism of sorbitol by P. vagans C9-1 may be a major metabolic feature in biocontrol of fire blight. Biosynthetic genes for the antibacterial peptide pantocin A were found on a chromosomal 28-kb genomic island, and for dapdiamide E on the plasmid pPag2. There was no evidence of potential virulence factors that could enable an animal or phytopathogenic lifestyle and no indication of any genetic-based biosafety risk in the antagonist. CONCLUSIONS: Identifying key determinants contributing to disease suppression allows the development of procedures to follow their expression in planta and the genome sequence contributes to rationale risk assessment regarding the use of the biocontrol strain in agricultural systems.
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spelling pubmed-31376372011-07-25 Metabolic Versatility and Antibacterial Metabolite Biosynthesis Are Distinguishing Genomic Features of the Fire Blight Antagonist Pantoea vagans C9-1 Smits, Theo H. M. Rezzonico, Fabio Kamber, Tim Blom, Jochen Goesmann, Alexander Ishimaru, Carol A. Frey, Jürg E. Stockwell, Virginia O. Duffy, Brion PLoS One Research Article BACKGROUND: Pantoea vagans is a commercialized biological control agent used against the pome fruit bacterial disease fire blight, caused by Erwinia amylovora. Compared to other biocontrol agents, relatively little is currently known regarding Pantoea genetics. Better understanding of antagonist mechanisms of action and ecological fitness is critical to improving efficacy. PRINCIPAL FINDINGS: Genome analysis indicated two major factors contribute to biocontrol activity: competition for limiting substrates and antibacterial metabolite production. Pathways for utilization of a broad diversity of sugars and acquisition of iron were identified. Metabolism of sorbitol by P. vagans C9-1 may be a major metabolic feature in biocontrol of fire blight. Biosynthetic genes for the antibacterial peptide pantocin A were found on a chromosomal 28-kb genomic island, and for dapdiamide E on the plasmid pPag2. There was no evidence of potential virulence factors that could enable an animal or phytopathogenic lifestyle and no indication of any genetic-based biosafety risk in the antagonist. CONCLUSIONS: Identifying key determinants contributing to disease suppression allows the development of procedures to follow their expression in planta and the genome sequence contributes to rationale risk assessment regarding the use of the biocontrol strain in agricultural systems. Public Library of Science 2011-07-15 /pmc/articles/PMC3137637/ /pubmed/21789243 http://dx.doi.org/10.1371/journal.pone.0022247 Text en Smits et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Smits, Theo H. M.
Rezzonico, Fabio
Kamber, Tim
Blom, Jochen
Goesmann, Alexander
Ishimaru, Carol A.
Frey, Jürg E.
Stockwell, Virginia O.
Duffy, Brion
Metabolic Versatility and Antibacterial Metabolite Biosynthesis Are Distinguishing Genomic Features of the Fire Blight Antagonist Pantoea vagans C9-1
title Metabolic Versatility and Antibacterial Metabolite Biosynthesis Are Distinguishing Genomic Features of the Fire Blight Antagonist Pantoea vagans C9-1
title_full Metabolic Versatility and Antibacterial Metabolite Biosynthesis Are Distinguishing Genomic Features of the Fire Blight Antagonist Pantoea vagans C9-1
title_fullStr Metabolic Versatility and Antibacterial Metabolite Biosynthesis Are Distinguishing Genomic Features of the Fire Blight Antagonist Pantoea vagans C9-1
title_full_unstemmed Metabolic Versatility and Antibacterial Metabolite Biosynthesis Are Distinguishing Genomic Features of the Fire Blight Antagonist Pantoea vagans C9-1
title_short Metabolic Versatility and Antibacterial Metabolite Biosynthesis Are Distinguishing Genomic Features of the Fire Blight Antagonist Pantoea vagans C9-1
title_sort metabolic versatility and antibacterial metabolite biosynthesis are distinguishing genomic features of the fire blight antagonist pantoea vagans c9-1
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3137637/
https://www.ncbi.nlm.nih.gov/pubmed/21789243
http://dx.doi.org/10.1371/journal.pone.0022247
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