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Use of Immunohistochemistry Techniques in Patients Exposed to Sulphur Mustard Gas

We performed a pathologic study with further using an immunohistochemical technique (using anti-p63 and anti-CK5) on tissues obtained by open lung biopsy from 18 patients with previous exposure to sulphur mustard (SM) as case group and 8 unexposed patients (control group). The most frequent patholog...

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Autores principales: Ghanei, Mostafa, Chilosi, Marco, Mohammad Hosseini Akbari, Hassan, Motiei-Langroudi, Rouzbeh, Harandi, Ali Amini, Shamsaei, Hassan, Bahadori, Moslem, Tazelaar, Henry D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: SAGE-Hindawi Access to Research 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3138111/
https://www.ncbi.nlm.nih.gov/pubmed/21776342
http://dx.doi.org/10.4061/2011/659603
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author Ghanei, Mostafa
Chilosi, Marco
Mohammad Hosseini Akbari, Hassan
Motiei-Langroudi, Rouzbeh
Harandi, Ali Amini
Shamsaei, Hassan
Bahadori, Moslem
Tazelaar, Henry D.
author_facet Ghanei, Mostafa
Chilosi, Marco
Mohammad Hosseini Akbari, Hassan
Motiei-Langroudi, Rouzbeh
Harandi, Ali Amini
Shamsaei, Hassan
Bahadori, Moslem
Tazelaar, Henry D.
author_sort Ghanei, Mostafa
collection PubMed
description We performed a pathologic study with further using an immunohistochemical technique (using anti-p63 and anti-CK5) on tissues obtained by open lung biopsy from 18 patients with previous exposure to sulphur mustard (SM) as case group and 8 unexposed patients (control group). The most frequent pathologic diagnosis was constrictive bronchiolitis (44.4%), followed by respiratory (22.2%) and chronic cellular bronchiolitis (16.7%) in the case group, and hypersensitivity bronchiolitis (50%) in the control group. The pathologic diagnoses were significantly different in the case and control groups (P = 0.042). In slides stained by anti-p63 and anti-CK5, the percent of stained cells and the mean number of epithelial cells were lower in the case group in comparison to the control group. This difference was significant for the mean number of cells stained by anti-CK5 (P = 0.042). Furthermore, there was a significant correlation between pathologic diagnosis and total number of cells and mean number of cells stained with anti-p63 and anti-CK5 (P  value = 0.002, <0.001, 0.044). These results suggest that constrictive bronchiolitis may be the major pathologic consequence of exposure to SM. Moreover, decrease of p63 in respiratory tissues affected by SM may suggest the lack of regenerative capacity in these patients.
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spelling pubmed-31381112011-07-20 Use of Immunohistochemistry Techniques in Patients Exposed to Sulphur Mustard Gas Ghanei, Mostafa Chilosi, Marco Mohammad Hosseini Akbari, Hassan Motiei-Langroudi, Rouzbeh Harandi, Ali Amini Shamsaei, Hassan Bahadori, Moslem Tazelaar, Henry D. Patholog Res Int Research Article We performed a pathologic study with further using an immunohistochemical technique (using anti-p63 and anti-CK5) on tissues obtained by open lung biopsy from 18 patients with previous exposure to sulphur mustard (SM) as case group and 8 unexposed patients (control group). The most frequent pathologic diagnosis was constrictive bronchiolitis (44.4%), followed by respiratory (22.2%) and chronic cellular bronchiolitis (16.7%) in the case group, and hypersensitivity bronchiolitis (50%) in the control group. The pathologic diagnoses were significantly different in the case and control groups (P = 0.042). In slides stained by anti-p63 and anti-CK5, the percent of stained cells and the mean number of epithelial cells were lower in the case group in comparison to the control group. This difference was significant for the mean number of cells stained by anti-CK5 (P = 0.042). Furthermore, there was a significant correlation between pathologic diagnosis and total number of cells and mean number of cells stained with anti-p63 and anti-CK5 (P  value = 0.002, <0.001, 0.044). These results suggest that constrictive bronchiolitis may be the major pathologic consequence of exposure to SM. Moreover, decrease of p63 in respiratory tissues affected by SM may suggest the lack of regenerative capacity in these patients. SAGE-Hindawi Access to Research 2011-07-06 /pmc/articles/PMC3138111/ /pubmed/21776342 http://dx.doi.org/10.4061/2011/659603 Text en Copyright © 2011 Mostafa Ghanei et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Ghanei, Mostafa
Chilosi, Marco
Mohammad Hosseini Akbari, Hassan
Motiei-Langroudi, Rouzbeh
Harandi, Ali Amini
Shamsaei, Hassan
Bahadori, Moslem
Tazelaar, Henry D.
Use of Immunohistochemistry Techniques in Patients Exposed to Sulphur Mustard Gas
title Use of Immunohistochemistry Techniques in Patients Exposed to Sulphur Mustard Gas
title_full Use of Immunohistochemistry Techniques in Patients Exposed to Sulphur Mustard Gas
title_fullStr Use of Immunohistochemistry Techniques in Patients Exposed to Sulphur Mustard Gas
title_full_unstemmed Use of Immunohistochemistry Techniques in Patients Exposed to Sulphur Mustard Gas
title_short Use of Immunohistochemistry Techniques in Patients Exposed to Sulphur Mustard Gas
title_sort use of immunohistochemistry techniques in patients exposed to sulphur mustard gas
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3138111/
https://www.ncbi.nlm.nih.gov/pubmed/21776342
http://dx.doi.org/10.4061/2011/659603
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