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Histone Deacetylase Inhibition Enhances Self Renewal and Cardioprotection by Human Cord Blood-Derived CD34(+) Cells
BACKGROUND: Use of peripheral blood- or bone marrow-derived progenitors for ischemic heart repair is a feasible option to induce neo-vascularization in ischemic tissues. These cells, named Endothelial Progenitors Cells (EPCs), have been extensively characterized phenotypically and functionally. The...
Autores principales: | , , , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3138768/ https://www.ncbi.nlm.nih.gov/pubmed/21789227 http://dx.doi.org/10.1371/journal.pone.0022158 |
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author | Burba, Ilaria Colombo, Gualtiero I. Staszewsky, Lidia Irene De Simone, Marco Devanna, Paolo Nanni, Simona Avitabile, Daniele Molla, Fabiola Cosentino, Simona Russo, Ilaria De Angelis, Noeleen Soldo, Annarita Biondi, Antonella Gambini, Elisa Gaetano, Carlo Farsetti, Antonella Pompilio, Giulio Latini, Roberto Capogrossi, Maurizio C. Pesce, Maurizio |
author_facet | Burba, Ilaria Colombo, Gualtiero I. Staszewsky, Lidia Irene De Simone, Marco Devanna, Paolo Nanni, Simona Avitabile, Daniele Molla, Fabiola Cosentino, Simona Russo, Ilaria De Angelis, Noeleen Soldo, Annarita Biondi, Antonella Gambini, Elisa Gaetano, Carlo Farsetti, Antonella Pompilio, Giulio Latini, Roberto Capogrossi, Maurizio C. Pesce, Maurizio |
author_sort | Burba, Ilaria |
collection | PubMed |
description | BACKGROUND: Use of peripheral blood- or bone marrow-derived progenitors for ischemic heart repair is a feasible option to induce neo-vascularization in ischemic tissues. These cells, named Endothelial Progenitors Cells (EPCs), have been extensively characterized phenotypically and functionally. The clinical efficacy of cardiac repair by EPCs cells remains, however, limited, due to cell autonomous defects as a consequence of risk factors. The devise of “enhancement” strategies has been therefore sought to improve repair ability of these cells and increase the clinical benefit. PRINCIPAL FINDINGS: Pharmacologic inhibition of histone deacetylases (HDACs) is known to enhance hematopoietic stem cells engraftment by improvement of self renewal and inhibition of differentiation in the presence of mitogenic stimuli in vitro. In the present study cord blood-derived CD34(+) were pre-conditioned with the HDAC inhibitor Valproic Acid. This treatment affected stem cell growth and gene expression, and improved ischemic myocardium protection in an immunodeficient mouse model of myocardial infarction. CONCLUSIONS: Our results show that HDAC blockade leads to phenotype changes in CD34(+) cells with enhanced self renewal and cardioprotection. |
format | Online Article Text |
id | pubmed-3138768 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-31387682011-07-25 Histone Deacetylase Inhibition Enhances Self Renewal and Cardioprotection by Human Cord Blood-Derived CD34(+) Cells Burba, Ilaria Colombo, Gualtiero I. Staszewsky, Lidia Irene De Simone, Marco Devanna, Paolo Nanni, Simona Avitabile, Daniele Molla, Fabiola Cosentino, Simona Russo, Ilaria De Angelis, Noeleen Soldo, Annarita Biondi, Antonella Gambini, Elisa Gaetano, Carlo Farsetti, Antonella Pompilio, Giulio Latini, Roberto Capogrossi, Maurizio C. Pesce, Maurizio PLoS One Research Article BACKGROUND: Use of peripheral blood- or bone marrow-derived progenitors for ischemic heart repair is a feasible option to induce neo-vascularization in ischemic tissues. These cells, named Endothelial Progenitors Cells (EPCs), have been extensively characterized phenotypically and functionally. The clinical efficacy of cardiac repair by EPCs cells remains, however, limited, due to cell autonomous defects as a consequence of risk factors. The devise of “enhancement” strategies has been therefore sought to improve repair ability of these cells and increase the clinical benefit. PRINCIPAL FINDINGS: Pharmacologic inhibition of histone deacetylases (HDACs) is known to enhance hematopoietic stem cells engraftment by improvement of self renewal and inhibition of differentiation in the presence of mitogenic stimuli in vitro. In the present study cord blood-derived CD34(+) were pre-conditioned with the HDAC inhibitor Valproic Acid. This treatment affected stem cell growth and gene expression, and improved ischemic myocardium protection in an immunodeficient mouse model of myocardial infarction. CONCLUSIONS: Our results show that HDAC blockade leads to phenotype changes in CD34(+) cells with enhanced self renewal and cardioprotection. Public Library of Science 2011-07-18 /pmc/articles/PMC3138768/ /pubmed/21789227 http://dx.doi.org/10.1371/journal.pone.0022158 Text en Burba et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Burba, Ilaria Colombo, Gualtiero I. Staszewsky, Lidia Irene De Simone, Marco Devanna, Paolo Nanni, Simona Avitabile, Daniele Molla, Fabiola Cosentino, Simona Russo, Ilaria De Angelis, Noeleen Soldo, Annarita Biondi, Antonella Gambini, Elisa Gaetano, Carlo Farsetti, Antonella Pompilio, Giulio Latini, Roberto Capogrossi, Maurizio C. Pesce, Maurizio Histone Deacetylase Inhibition Enhances Self Renewal and Cardioprotection by Human Cord Blood-Derived CD34(+) Cells |
title | Histone Deacetylase Inhibition Enhances Self Renewal and Cardioprotection by Human Cord Blood-Derived CD34(+) Cells |
title_full | Histone Deacetylase Inhibition Enhances Self Renewal and Cardioprotection by Human Cord Blood-Derived CD34(+) Cells |
title_fullStr | Histone Deacetylase Inhibition Enhances Self Renewal and Cardioprotection by Human Cord Blood-Derived CD34(+) Cells |
title_full_unstemmed | Histone Deacetylase Inhibition Enhances Self Renewal and Cardioprotection by Human Cord Blood-Derived CD34(+) Cells |
title_short | Histone Deacetylase Inhibition Enhances Self Renewal and Cardioprotection by Human Cord Blood-Derived CD34(+) Cells |
title_sort | histone deacetylase inhibition enhances self renewal and cardioprotection by human cord blood-derived cd34(+) cells |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3138768/ https://www.ncbi.nlm.nih.gov/pubmed/21789227 http://dx.doi.org/10.1371/journal.pone.0022158 |
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