Cargando…

Validation of a Diagnostic Microarray for Human Papillomavirus: Coverage of 102 Genotypes

Papillomaviruses have been implicated in a variety of human diseases ranging from common warts to invasive carcinoma of the anogenital mucosa. Existing assays for genotyping human papillomavirus are restricted to a small number of types. Here, we present a comprehensive, accurate microarray strategy...

Descripción completa

Detalles Bibliográficos
Autores principales: Arron, Sarah Tuttleton, Skewes-Cox, Peter, Do, Phong H., Dybbro, Eric, Da Costa, Maria, Palefsky, Joel M., DeRisi, Joseph L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: SAGE-Hindawi Access to Research 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3139178/
https://www.ncbi.nlm.nih.gov/pubmed/21785699
http://dx.doi.org/10.4061/2011/756905
_version_ 1782208430830780416
author Arron, Sarah Tuttleton
Skewes-Cox, Peter
Do, Phong H.
Dybbro, Eric
Da Costa, Maria
Palefsky, Joel M.
DeRisi, Joseph L.
author_facet Arron, Sarah Tuttleton
Skewes-Cox, Peter
Do, Phong H.
Dybbro, Eric
Da Costa, Maria
Palefsky, Joel M.
DeRisi, Joseph L.
author_sort Arron, Sarah Tuttleton
collection PubMed
description Papillomaviruses have been implicated in a variety of human diseases ranging from common warts to invasive carcinoma of the anogenital mucosa. Existing assays for genotyping human papillomavirus are restricted to a small number of types. Here, we present a comprehensive, accurate microarray strategy for detection and genotyping of 102 human papillomavirus types and validate its use in a panel of 91 anal swabs. This array has equal performance to traditional dot blot analysis with the benefits of added genotype coverage and the ability to calibrate readout over a range of sensitivity or specificity values.
format Online
Article
Text
id pubmed-3139178
institution National Center for Biotechnology Information
language English
publishDate 2011
publisher SAGE-Hindawi Access to Research
record_format MEDLINE/PubMed
spelling pubmed-31391782011-07-22 Validation of a Diagnostic Microarray for Human Papillomavirus: Coverage of 102 Genotypes Arron, Sarah Tuttleton Skewes-Cox, Peter Do, Phong H. Dybbro, Eric Da Costa, Maria Palefsky, Joel M. DeRisi, Joseph L. J Nucleic Acids Research Article Papillomaviruses have been implicated in a variety of human diseases ranging from common warts to invasive carcinoma of the anogenital mucosa. Existing assays for genotyping human papillomavirus are restricted to a small number of types. Here, we present a comprehensive, accurate microarray strategy for detection and genotyping of 102 human papillomavirus types and validate its use in a panel of 91 anal swabs. This array has equal performance to traditional dot blot analysis with the benefits of added genotype coverage and the ability to calibrate readout over a range of sensitivity or specificity values. SAGE-Hindawi Access to Research 2011-05-18 /pmc/articles/PMC3139178/ /pubmed/21785699 http://dx.doi.org/10.4061/2011/756905 Text en Copyright © 2011 Sarah Tuttleton Arron et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Arron, Sarah Tuttleton
Skewes-Cox, Peter
Do, Phong H.
Dybbro, Eric
Da Costa, Maria
Palefsky, Joel M.
DeRisi, Joseph L.
Validation of a Diagnostic Microarray for Human Papillomavirus: Coverage of 102 Genotypes
title Validation of a Diagnostic Microarray for Human Papillomavirus: Coverage of 102 Genotypes
title_full Validation of a Diagnostic Microarray for Human Papillomavirus: Coverage of 102 Genotypes
title_fullStr Validation of a Diagnostic Microarray for Human Papillomavirus: Coverage of 102 Genotypes
title_full_unstemmed Validation of a Diagnostic Microarray for Human Papillomavirus: Coverage of 102 Genotypes
title_short Validation of a Diagnostic Microarray for Human Papillomavirus: Coverage of 102 Genotypes
title_sort validation of a diagnostic microarray for human papillomavirus: coverage of 102 genotypes
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3139178/
https://www.ncbi.nlm.nih.gov/pubmed/21785699
http://dx.doi.org/10.4061/2011/756905
work_keys_str_mv AT arronsarahtuttleton validationofadiagnosticmicroarrayforhumanpapillomaviruscoverageof102genotypes
AT skewescoxpeter validationofadiagnosticmicroarrayforhumanpapillomaviruscoverageof102genotypes
AT dophongh validationofadiagnosticmicroarrayforhumanpapillomaviruscoverageof102genotypes
AT dybbroeric validationofadiagnosticmicroarrayforhumanpapillomaviruscoverageof102genotypes
AT dacostamaria validationofadiagnosticmicroarrayforhumanpapillomaviruscoverageof102genotypes
AT palefskyjoelm validationofadiagnosticmicroarrayforhumanpapillomaviruscoverageof102genotypes
AT derisijosephl validationofadiagnosticmicroarrayforhumanpapillomaviruscoverageof102genotypes