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Validation of a Diagnostic Microarray for Human Papillomavirus: Coverage of 102 Genotypes
Papillomaviruses have been implicated in a variety of human diseases ranging from common warts to invasive carcinoma of the anogenital mucosa. Existing assays for genotyping human papillomavirus are restricted to a small number of types. Here, we present a comprehensive, accurate microarray strategy...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
SAGE-Hindawi Access to Research
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3139178/ https://www.ncbi.nlm.nih.gov/pubmed/21785699 http://dx.doi.org/10.4061/2011/756905 |
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author | Arron, Sarah Tuttleton Skewes-Cox, Peter Do, Phong H. Dybbro, Eric Da Costa, Maria Palefsky, Joel M. DeRisi, Joseph L. |
author_facet | Arron, Sarah Tuttleton Skewes-Cox, Peter Do, Phong H. Dybbro, Eric Da Costa, Maria Palefsky, Joel M. DeRisi, Joseph L. |
author_sort | Arron, Sarah Tuttleton |
collection | PubMed |
description | Papillomaviruses have been implicated in a variety of human diseases ranging from common warts to invasive carcinoma of the anogenital mucosa. Existing assays for genotyping human papillomavirus are restricted to a small number of types. Here, we present a comprehensive, accurate microarray strategy for detection and genotyping of 102 human papillomavirus types and validate its use in a panel of 91 anal swabs. This array has equal performance to traditional dot blot analysis with the benefits of added genotype coverage and the ability to calibrate readout over a range of sensitivity or specificity values. |
format | Online Article Text |
id | pubmed-3139178 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | SAGE-Hindawi Access to Research |
record_format | MEDLINE/PubMed |
spelling | pubmed-31391782011-07-22 Validation of a Diagnostic Microarray for Human Papillomavirus: Coverage of 102 Genotypes Arron, Sarah Tuttleton Skewes-Cox, Peter Do, Phong H. Dybbro, Eric Da Costa, Maria Palefsky, Joel M. DeRisi, Joseph L. J Nucleic Acids Research Article Papillomaviruses have been implicated in a variety of human diseases ranging from common warts to invasive carcinoma of the anogenital mucosa. Existing assays for genotyping human papillomavirus are restricted to a small number of types. Here, we present a comprehensive, accurate microarray strategy for detection and genotyping of 102 human papillomavirus types and validate its use in a panel of 91 anal swabs. This array has equal performance to traditional dot blot analysis with the benefits of added genotype coverage and the ability to calibrate readout over a range of sensitivity or specificity values. SAGE-Hindawi Access to Research 2011-05-18 /pmc/articles/PMC3139178/ /pubmed/21785699 http://dx.doi.org/10.4061/2011/756905 Text en Copyright © 2011 Sarah Tuttleton Arron et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Arron, Sarah Tuttleton Skewes-Cox, Peter Do, Phong H. Dybbro, Eric Da Costa, Maria Palefsky, Joel M. DeRisi, Joseph L. Validation of a Diagnostic Microarray for Human Papillomavirus: Coverage of 102 Genotypes |
title | Validation of a Diagnostic Microarray for Human Papillomavirus: Coverage of 102 Genotypes |
title_full | Validation of a Diagnostic Microarray for Human Papillomavirus: Coverage of 102 Genotypes |
title_fullStr | Validation of a Diagnostic Microarray for Human Papillomavirus: Coverage of 102 Genotypes |
title_full_unstemmed | Validation of a Diagnostic Microarray for Human Papillomavirus: Coverage of 102 Genotypes |
title_short | Validation of a Diagnostic Microarray for Human Papillomavirus: Coverage of 102 Genotypes |
title_sort | validation of a diagnostic microarray for human papillomavirus: coverage of 102 genotypes |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3139178/ https://www.ncbi.nlm.nih.gov/pubmed/21785699 http://dx.doi.org/10.4061/2011/756905 |
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