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Microinjection Manipulation Resulted in the Increased Apoptosis of Spermatocytes in Testes from Intracytoplasmic Sperm Injection (ICSI) Derived Mice

The invention of intracytoplasmic sperm injection (ICSI) has possibly been the most important development in reproductive medicine, one that has given hope to thousands of infertile couples worldwide. However, concerns remain regarding the safety of this method since it is a more invasive procedure...

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Autores principales: Yu, Yang, Zhao, Chun, Lv, Zhuo, Chen, Wen, Tong, Man, Guo, Xuejiang, Wang, Liu, Liu, Jiayin, Zhou, Zuomin, Zhu, Hui, Zhou, Qi, Sha, Jiahao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3140508/
https://www.ncbi.nlm.nih.gov/pubmed/21799787
http://dx.doi.org/10.1371/journal.pone.0022172
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author Yu, Yang
Zhao, Chun
Lv, Zhuo
Chen, Wen
Tong, Man
Guo, Xuejiang
Wang, Liu
Liu, Jiayin
Zhou, Zuomin
Zhu, Hui
Zhou, Qi
Sha, Jiahao
author_facet Yu, Yang
Zhao, Chun
Lv, Zhuo
Chen, Wen
Tong, Man
Guo, Xuejiang
Wang, Liu
Liu, Jiayin
Zhou, Zuomin
Zhu, Hui
Zhou, Qi
Sha, Jiahao
author_sort Yu, Yang
collection PubMed
description The invention of intracytoplasmic sperm injection (ICSI) has possibly been the most important development in reproductive medicine, one that has given hope to thousands of infertile couples worldwide. However, concerns remain regarding the safety of this method since it is a more invasive procedure than in vitro fertilization (IVF), since a spermatozoon is injected into the oocyte cytoplasm. Using mice derived from IVF technology as a control, we assessed the influence of invasive microinjection in the process of transferring sperm into oocyte cytoplasm in ICSI procedure on the development and physiologic function of resultant offspring. Our results demonstrated that mice produced from ICSI and IVF had no significant difference in phenotypic indices including body weight, forelimb physiology, and learning and memory ability. However, increased spermatocyte apoptosis was observed in the testis of adult ICSI mice, when compared with IVF mice. And, decreased testis weight and marked damage of spermatogenic epithelia were found in aged ICSI mice. Furthermore, proteomic analysis verified that most of the differentiated proteins in testes between adult ICSI and IVF mice were those involved in regulation of apoptosis pathways. Our results demonstrated that the microinjection manipulation used in the ICSI procedure might pose potential risks to the fertility of male offspring. The changed expression of a series of proteins relating to apoptosis or proliferation might contribute to it. Further studies are necessary to better understand all the risks of ICSI.
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spelling pubmed-31405082011-07-28 Microinjection Manipulation Resulted in the Increased Apoptosis of Spermatocytes in Testes from Intracytoplasmic Sperm Injection (ICSI) Derived Mice Yu, Yang Zhao, Chun Lv, Zhuo Chen, Wen Tong, Man Guo, Xuejiang Wang, Liu Liu, Jiayin Zhou, Zuomin Zhu, Hui Zhou, Qi Sha, Jiahao PLoS One Research Article The invention of intracytoplasmic sperm injection (ICSI) has possibly been the most important development in reproductive medicine, one that has given hope to thousands of infertile couples worldwide. However, concerns remain regarding the safety of this method since it is a more invasive procedure than in vitro fertilization (IVF), since a spermatozoon is injected into the oocyte cytoplasm. Using mice derived from IVF technology as a control, we assessed the influence of invasive microinjection in the process of transferring sperm into oocyte cytoplasm in ICSI procedure on the development and physiologic function of resultant offspring. Our results demonstrated that mice produced from ICSI and IVF had no significant difference in phenotypic indices including body weight, forelimb physiology, and learning and memory ability. However, increased spermatocyte apoptosis was observed in the testis of adult ICSI mice, when compared with IVF mice. And, decreased testis weight and marked damage of spermatogenic epithelia were found in aged ICSI mice. Furthermore, proteomic analysis verified that most of the differentiated proteins in testes between adult ICSI and IVF mice were those involved in regulation of apoptosis pathways. Our results demonstrated that the microinjection manipulation used in the ICSI procedure might pose potential risks to the fertility of male offspring. The changed expression of a series of proteins relating to apoptosis or proliferation might contribute to it. Further studies are necessary to better understand all the risks of ICSI. Public Library of Science 2011-07-20 /pmc/articles/PMC3140508/ /pubmed/21799787 http://dx.doi.org/10.1371/journal.pone.0022172 Text en Yu et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Yu, Yang
Zhao, Chun
Lv, Zhuo
Chen, Wen
Tong, Man
Guo, Xuejiang
Wang, Liu
Liu, Jiayin
Zhou, Zuomin
Zhu, Hui
Zhou, Qi
Sha, Jiahao
Microinjection Manipulation Resulted in the Increased Apoptosis of Spermatocytes in Testes from Intracytoplasmic Sperm Injection (ICSI) Derived Mice
title Microinjection Manipulation Resulted in the Increased Apoptosis of Spermatocytes in Testes from Intracytoplasmic Sperm Injection (ICSI) Derived Mice
title_full Microinjection Manipulation Resulted in the Increased Apoptosis of Spermatocytes in Testes from Intracytoplasmic Sperm Injection (ICSI) Derived Mice
title_fullStr Microinjection Manipulation Resulted in the Increased Apoptosis of Spermatocytes in Testes from Intracytoplasmic Sperm Injection (ICSI) Derived Mice
title_full_unstemmed Microinjection Manipulation Resulted in the Increased Apoptosis of Spermatocytes in Testes from Intracytoplasmic Sperm Injection (ICSI) Derived Mice
title_short Microinjection Manipulation Resulted in the Increased Apoptosis of Spermatocytes in Testes from Intracytoplasmic Sperm Injection (ICSI) Derived Mice
title_sort microinjection manipulation resulted in the increased apoptosis of spermatocytes in testes from intracytoplasmic sperm injection (icsi) derived mice
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3140508/
https://www.ncbi.nlm.nih.gov/pubmed/21799787
http://dx.doi.org/10.1371/journal.pone.0022172
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