Cargando…

Expression of DOG1, PDGFRA, and p16 in Gastrointestinal Stromal Tumors

BACKGROUND/AIMS: The diagnosis of gastrointestinal stromal tumors (GIST) relies on the demonstration of KIT expression, but KIT expression is absent or reduced in approximately 15% of GIST. METHODS: Eighty-one GISTs were diagnosed between January 1998 and December 2007 at the Department of Pathology...

Descripción completa

Detalles Bibliográficos
Autores principales: Jung, Sung Hee, Suh, Kwang Sun, Kang, Dae Young, Kang, Dong Wook, Kim, Young-Beum, Kim, Eun-Sun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Korean Society of Gastroenterology; the Korean Society of Gastrointestinal Endoscopy; the Korean Association for the Study of the Liver; the Korean Society of Neurogastroenterology and Motility; Korean Association for the Study of Intestinal Diseases; Korean College of Helicobacter and Upper Gastrointestinal Research; Korean Society of Pancreatobiliary Diseases 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3140662/
https://www.ncbi.nlm.nih.gov/pubmed/21814597
http://dx.doi.org/10.5009/gnl.2011.5.2.171
_version_ 1782208578426241024
author Jung, Sung Hee
Suh, Kwang Sun
Kang, Dae Young
Kang, Dong Wook
Kim, Young-Beum
Kim, Eun-Sun
author_facet Jung, Sung Hee
Suh, Kwang Sun
Kang, Dae Young
Kang, Dong Wook
Kim, Young-Beum
Kim, Eun-Sun
author_sort Jung, Sung Hee
collection PubMed
description BACKGROUND/AIMS: The diagnosis of gastrointestinal stromal tumors (GIST) relies on the demonstration of KIT expression, but KIT expression is absent or reduced in approximately 15% of GIST. METHODS: Eighty-one GISTs were diagnosed between January 1998 and December 2007 at the Department of Pathology at both Chungnam National University Hospital and Eulji University Hospital, Daejeon. Medical history, patient follow-up, and radiographic data were collected if available in the medical records. To determine diagnostic and prognostic markers for GISTs focused on PDGFRA mutation and clinicopathologic features, we analyzed 81 GIST cases for KIT, PDGFRA, DOG1, and p16 expression and for mutation of PDGFRA genes. RESULTS: Among 81 GIST cases, 20 high risk cases (24.7%) were recurred or metastasized. Immunohistochemically, KIT was positive in 76 (93.8%), PDGFRA in 75 (92.7%), and DOG1 in 77 (95.1%). With a cutoff value of 50%, p16 expression was positive in 26 cases were positive (32.1%). A correlation between p16 expression or negative DOG1 expression and recurrence or metastasis was demonstrated (p<0.05). Four cases showed a missense mutation in exon 12 of PDGFRA gene, three of these were of epithelioid GISTs. Two cases showed a silent mutation in exon 18 of PDGFRA. CONCLUSIONS: These results indicate that the expression of DOG1 and PDGFRA is observed in a majority of GIST cases. Expression of p16 and negative DOG1 expression is predictive for development of recurrence and/or metastasis. Even though mutation of the PDGFRA gene is frequently seen in epithelioid GISTs, a clinicopathologic correlation was not demonstrated.
format Online
Article
Text
id pubmed-3140662
institution National Center for Biotechnology Information
language English
publishDate 2011
publisher The Korean Society of Gastroenterology; the Korean Society of Gastrointestinal Endoscopy; the Korean Association for the Study of the Liver; the Korean Society of Neurogastroenterology and Motility; Korean Association for the Study of Intestinal Diseases; Korean College of Helicobacter and Upper Gastrointestinal Research; Korean Society of Pancreatobiliary Diseases
record_format MEDLINE/PubMed
spelling pubmed-31406622011-08-03 Expression of DOG1, PDGFRA, and p16 in Gastrointestinal Stromal Tumors Jung, Sung Hee Suh, Kwang Sun Kang, Dae Young Kang, Dong Wook Kim, Young-Beum Kim, Eun-Sun Gut Liver Original Article BACKGROUND/AIMS: The diagnosis of gastrointestinal stromal tumors (GIST) relies on the demonstration of KIT expression, but KIT expression is absent or reduced in approximately 15% of GIST. METHODS: Eighty-one GISTs were diagnosed between January 1998 and December 2007 at the Department of Pathology at both Chungnam National University Hospital and Eulji University Hospital, Daejeon. Medical history, patient follow-up, and radiographic data were collected if available in the medical records. To determine diagnostic and prognostic markers for GISTs focused on PDGFRA mutation and clinicopathologic features, we analyzed 81 GIST cases for KIT, PDGFRA, DOG1, and p16 expression and for mutation of PDGFRA genes. RESULTS: Among 81 GIST cases, 20 high risk cases (24.7%) were recurred or metastasized. Immunohistochemically, KIT was positive in 76 (93.8%), PDGFRA in 75 (92.7%), and DOG1 in 77 (95.1%). With a cutoff value of 50%, p16 expression was positive in 26 cases were positive (32.1%). A correlation between p16 expression or negative DOG1 expression and recurrence or metastasis was demonstrated (p<0.05). Four cases showed a missense mutation in exon 12 of PDGFRA gene, three of these were of epithelioid GISTs. Two cases showed a silent mutation in exon 18 of PDGFRA. CONCLUSIONS: These results indicate that the expression of DOG1 and PDGFRA is observed in a majority of GIST cases. Expression of p16 and negative DOG1 expression is predictive for development of recurrence and/or metastasis. Even though mutation of the PDGFRA gene is frequently seen in epithelioid GISTs, a clinicopathologic correlation was not demonstrated. The Korean Society of Gastroenterology; the Korean Society of Gastrointestinal Endoscopy; the Korean Association for the Study of the Liver; the Korean Society of Neurogastroenterology and Motility; Korean Association for the Study of Intestinal Diseases; Korean College of Helicobacter and Upper Gastrointestinal Research; Korean Society of Pancreatobiliary Diseases 2011-06 2011-06-24 /pmc/articles/PMC3140662/ /pubmed/21814597 http://dx.doi.org/10.5009/gnl.2011.5.2.171 Text en Copyright © 2011 The Korean Society of Gastroenterology, the Korean Society of Gastrointestinal Endoscopy, the Korean Society of Neurogastroenterology and Motility, Korean College of Helicobacter and Upper Gastrointestinal Research, the Korean Association for the Study of Intestinal Diseases, Korean Association for the Study of the Liver and Korean Society of Pancreatobiliary Diseases http://creativecommons.org/licenses/by-nc/3.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Jung, Sung Hee
Suh, Kwang Sun
Kang, Dae Young
Kang, Dong Wook
Kim, Young-Beum
Kim, Eun-Sun
Expression of DOG1, PDGFRA, and p16 in Gastrointestinal Stromal Tumors
title Expression of DOG1, PDGFRA, and p16 in Gastrointestinal Stromal Tumors
title_full Expression of DOG1, PDGFRA, and p16 in Gastrointestinal Stromal Tumors
title_fullStr Expression of DOG1, PDGFRA, and p16 in Gastrointestinal Stromal Tumors
title_full_unstemmed Expression of DOG1, PDGFRA, and p16 in Gastrointestinal Stromal Tumors
title_short Expression of DOG1, PDGFRA, and p16 in Gastrointestinal Stromal Tumors
title_sort expression of dog1, pdgfra, and p16 in gastrointestinal stromal tumors
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3140662/
https://www.ncbi.nlm.nih.gov/pubmed/21814597
http://dx.doi.org/10.5009/gnl.2011.5.2.171
work_keys_str_mv AT jungsunghee expressionofdog1pdgfraandp16ingastrointestinalstromaltumors
AT suhkwangsun expressionofdog1pdgfraandp16ingastrointestinalstromaltumors
AT kangdaeyoung expressionofdog1pdgfraandp16ingastrointestinalstromaltumors
AT kangdongwook expressionofdog1pdgfraandp16ingastrointestinalstromaltumors
AT kimyoungbeum expressionofdog1pdgfraandp16ingastrointestinalstromaltumors
AT kimeunsun expressionofdog1pdgfraandp16ingastrointestinalstromaltumors