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Selection of Resistant Bacteria at Very Low Antibiotic Concentrations
The widespread use of antibiotics is selecting for a variety of resistance mechanisms that seriously challenge our ability to treat bacterial infections. Resistant bacteria can be selected at the high concentrations of antibiotics used therapeutically, but what role the much lower antibiotic concent...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3141051/ https://www.ncbi.nlm.nih.gov/pubmed/21811410 http://dx.doi.org/10.1371/journal.ppat.1002158 |
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author | Gullberg, Erik Cao, Sha Berg, Otto G. Ilbäck, Carolina Sandegren, Linus Hughes, Diarmaid Andersson, Dan I. |
author_facet | Gullberg, Erik Cao, Sha Berg, Otto G. Ilbäck, Carolina Sandegren, Linus Hughes, Diarmaid Andersson, Dan I. |
author_sort | Gullberg, Erik |
collection | PubMed |
description | The widespread use of antibiotics is selecting for a variety of resistance mechanisms that seriously challenge our ability to treat bacterial infections. Resistant bacteria can be selected at the high concentrations of antibiotics used therapeutically, but what role the much lower antibiotic concentrations present in many environments plays in selection remains largely unclear. Here we show using highly sensitive competition experiments that selection of resistant bacteria occurs at extremely low antibiotic concentrations. Thus, for three clinically important antibiotics, drug concentrations up to several hundred-fold below the minimal inhibitory concentration of susceptible bacteria could enrich for resistant bacteria, even when present at a very low initial fraction. We also show that de novo mutants can be selected at sub-MIC concentrations of antibiotics, and we provide a mathematical model predicting how rapidly such mutants would take over in a susceptible population. These results add another dimension to the evolution of resistance and suggest that the low antibiotic concentrations found in many natural environments are important for enrichment and maintenance of resistance in bacterial populations. |
format | Online Article Text |
id | pubmed-3141051 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-31410512011-08-02 Selection of Resistant Bacteria at Very Low Antibiotic Concentrations Gullberg, Erik Cao, Sha Berg, Otto G. Ilbäck, Carolina Sandegren, Linus Hughes, Diarmaid Andersson, Dan I. PLoS Pathog Research Article The widespread use of antibiotics is selecting for a variety of resistance mechanisms that seriously challenge our ability to treat bacterial infections. Resistant bacteria can be selected at the high concentrations of antibiotics used therapeutically, but what role the much lower antibiotic concentrations present in many environments plays in selection remains largely unclear. Here we show using highly sensitive competition experiments that selection of resistant bacteria occurs at extremely low antibiotic concentrations. Thus, for three clinically important antibiotics, drug concentrations up to several hundred-fold below the minimal inhibitory concentration of susceptible bacteria could enrich for resistant bacteria, even when present at a very low initial fraction. We also show that de novo mutants can be selected at sub-MIC concentrations of antibiotics, and we provide a mathematical model predicting how rapidly such mutants would take over in a susceptible population. These results add another dimension to the evolution of resistance and suggest that the low antibiotic concentrations found in many natural environments are important for enrichment and maintenance of resistance in bacterial populations. Public Library of Science 2011-07-21 /pmc/articles/PMC3141051/ /pubmed/21811410 http://dx.doi.org/10.1371/journal.ppat.1002158 Text en Gullberg et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Gullberg, Erik Cao, Sha Berg, Otto G. Ilbäck, Carolina Sandegren, Linus Hughes, Diarmaid Andersson, Dan I. Selection of Resistant Bacteria at Very Low Antibiotic Concentrations |
title | Selection of Resistant Bacteria at Very Low Antibiotic Concentrations |
title_full | Selection of Resistant Bacteria at Very Low Antibiotic Concentrations |
title_fullStr | Selection of Resistant Bacteria at Very Low Antibiotic Concentrations |
title_full_unstemmed | Selection of Resistant Bacteria at Very Low Antibiotic Concentrations |
title_short | Selection of Resistant Bacteria at Very Low Antibiotic Concentrations |
title_sort | selection of resistant bacteria at very low antibiotic concentrations |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3141051/ https://www.ncbi.nlm.nih.gov/pubmed/21811410 http://dx.doi.org/10.1371/journal.ppat.1002158 |
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