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Paired mutations abolish and restore the balanced annealing and melting activities of ORF1p that are required for LINE-1 retrotransposition

Retrotransposition amplifies LINE-1 (L1) to high copy number in mammalian genomes. The L1 protein encoded by ORF1 (ORF1p) is required for retrotransposition. This dependence on ORF1p was investigated by mutating three highly conserved residues, R238, R284 and Y318 to alanine, thereby inactivating re...

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Detalles Bibliográficos
Autores principales:	Evans, James D., Peddigari, Suresh, Chaurasiya, Kathy R., Williams, Mark C., Martin, Sandra L.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Oxford University Press 2011
Materias:	Nucleic Acid Enzymes
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3141268/ https://www.ncbi.nlm.nih.gov/pubmed/21441536 http://dx.doi.org/10.1093/nar/gkr171

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