The parallel G-quadruplex structure of vertebrate telomeric repeat sequences is not the preferred folding topology under physiological conditions

G-quadruplex topologies of telomeric repeat sequences from vertebrates were investigated in the presence of molecular crowding (MC) mimetics, namely polyethylene glycol 200 (PEG), Ficoll 70 as well as Xenopus laevis egg extract by CD and NMR spectroscopy and native PAGE. Here, we show that the confo...

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Autores principales: Hänsel, Robert, Löhr, Frank, Foldynová-Trantírková, Silvie, Bamberg, Ernst, Trantírek, Lukáš, Dötsch, Volker
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2011
Materias:
Structural Biology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3141269/
https://www.ncbi.nlm.nih.gov/pubmed/21450807
http://dx.doi.org/10.1093/nar/gkr174
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author Hänsel, Robert
Löhr, Frank
Foldynová-Trantírková, Silvie
Bamberg, Ernst
Trantírek, Lukáš
Dötsch, Volker
author_facet Hänsel, Robert
Löhr, Frank
Foldynová-Trantírková, Silvie
Bamberg, Ernst
Trantírek, Lukáš
Dötsch, Volker
author_sort Hänsel, Robert
collection PubMed
description G-quadruplex topologies of telomeric repeat sequences from vertebrates were investigated in the presence of molecular crowding (MC) mimetics, namely polyethylene glycol 200 (PEG), Ficoll 70 as well as Xenopus laevis egg extract by CD and NMR spectroscopy and native PAGE. Here, we show that the conformational behavior of the telomeric repeats in X. laevis egg extract or in Ficoll is notably different from that observed in the presence of PEG. While the behavior of the telomeric repeat in X. laevis egg extract or in Ficoll resembles results obtained under dilute conditions, PEG promotes the formation of high-order parallel topologies. Our data suggest that PEG should not be used as a MC mimetic.
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spelling pubmed-31412692011-07-22 The parallel G-quadruplex structure of vertebrate telomeric repeat sequences is not the preferred folding topology under physiological conditions Hänsel, Robert Löhr, Frank Foldynová-Trantírková, Silvie Bamberg, Ernst Trantírek, Lukáš Dötsch, Volker Nucleic Acids Res Structural Biology G-quadruplex topologies of telomeric repeat sequences from vertebrates were investigated in the presence of molecular crowding (MC) mimetics, namely polyethylene glycol 200 (PEG), Ficoll 70 as well as Xenopus laevis egg extract by CD and NMR spectroscopy and native PAGE. Here, we show that the conformational behavior of the telomeric repeats in X. laevis egg extract or in Ficoll is notably different from that observed in the presence of PEG. While the behavior of the telomeric repeat in X. laevis egg extract or in Ficoll resembles results obtained under dilute conditions, PEG promotes the formation of high-order parallel topologies. Our data suggest that PEG should not be used as a MC mimetic. Oxford University Press 2011-07 2011-03-30 /pmc/articles/PMC3141269/ /pubmed/21450807 http://dx.doi.org/10.1093/nar/gkr174 Text en © The Author(s) 2011. Published by Oxford University Press. http://creativecommons.org/licenses/by-nc/2.5 This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Structural Biology
Hänsel, Robert
Löhr, Frank
Foldynová-Trantírková, Silvie
Bamberg, Ernst
Trantírek, Lukáš
Dötsch, Volker
The parallel G-quadruplex structure of vertebrate telomeric repeat sequences is not the preferred folding topology under physiological conditions
title The parallel G-quadruplex structure of vertebrate telomeric repeat sequences is not the preferred folding topology under physiological conditions
title_full The parallel G-quadruplex structure of vertebrate telomeric repeat sequences is not the preferred folding topology under physiological conditions
title_fullStr The parallel G-quadruplex structure of vertebrate telomeric repeat sequences is not the preferred folding topology under physiological conditions
title_full_unstemmed The parallel G-quadruplex structure of vertebrate telomeric repeat sequences is not the preferred folding topology under physiological conditions
title_short The parallel G-quadruplex structure of vertebrate telomeric repeat sequences is not the preferred folding topology under physiological conditions
title_sort parallel g-quadruplex structure of vertebrate telomeric repeat sequences is not the preferred folding topology under physiological conditions
topic Structural Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3141269/
https://www.ncbi.nlm.nih.gov/pubmed/21450807
http://dx.doi.org/10.1093/nar/gkr174
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