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An intein with genetically selectable markers provides a new approach to internally label proteins with GFP

BACKGROUND: Inteins are proteins that catalyze their own removal from within larger precursor proteins. In the process they splice the flanking protein sequences, termed the N-and C-terminal exteins. Large inteins frequently have a homing endonuclease that is involved in maintaining the intein in th...

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Detalles Bibliográficos
Autores principales: Ramsden, Richard, Arms, Luther, Davis, Trisha N, Muller, Eric GD
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3141402/
https://www.ncbi.nlm.nih.gov/pubmed/21708017
http://dx.doi.org/10.1186/1472-6750-11-71