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An intein with genetically selectable markers provides a new approach to internally label proteins with GFP
BACKGROUND: Inteins are proteins that catalyze their own removal from within larger precursor proteins. In the process they splice the flanking protein sequences, termed the N-and C-terminal exteins. Large inteins frequently have a homing endonuclease that is involved in maintaining the intein in th...
Autores principales: | Ramsden, Richard, Arms, Luther, Davis, Trisha N, Muller, Eric GD |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3141402/ https://www.ncbi.nlm.nih.gov/pubmed/21708017 http://dx.doi.org/10.1186/1472-6750-11-71 |
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