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Expression analysis of asthma candidate genes during human and murine lung development
BACKGROUND: Little is known about the role of most asthma susceptibility genes during human lung development. Genetic determinants for normal lung development are not only important early in life, but also for later lung function. OBJECTIVE: To investigate the role of expression patterns of well-def...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3141421/ https://www.ncbi.nlm.nih.gov/pubmed/21699702 http://dx.doi.org/10.1186/1465-9921-12-86 |
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author | Melén, Erik Kho, Alvin T Sharma, Sunita Gaedigk, Roger Leeder, J Steven Mariani, Thomas J Carey, Vincent J Weiss, Scott T Tantisira, Kelan G |
author_facet | Melén, Erik Kho, Alvin T Sharma, Sunita Gaedigk, Roger Leeder, J Steven Mariani, Thomas J Carey, Vincent J Weiss, Scott T Tantisira, Kelan G |
author_sort | Melén, Erik |
collection | PubMed |
description | BACKGROUND: Little is known about the role of most asthma susceptibility genes during human lung development. Genetic determinants for normal lung development are not only important early in life, but also for later lung function. OBJECTIVE: To investigate the role of expression patterns of well-defined asthma susceptibility genes during human and murine lung development. We hypothesized that genes influencing normal airways development would be over-represented by genes associated with asthma. METHODS: Asthma genes were first identified via comprehensive search of the current literature. Next, we analyzed their expression patterns in the developing human lung during the pseudoglandular (gestational age, 7-16 weeks) and canalicular (17-26 weeks) stages of development, and in the complete developing lung time series of 3 mouse strains: A/J, SW, C57BL6. RESULTS: In total, 96 genes with association to asthma in at least two human populations were identified in the literature. Overall, there was no significant over-representation of the asthma genes among genes differentially expressed during lung development, although trends were seen in the human (Odds ratio, OR 1.22, confidence interval, CI 0.90-1.62) and C57BL6 mouse (OR 1.41, CI 0.92-2.11) data. However, differential expression of some asthma genes was consistent in both developing human and murine lung, e.g. NOD1, EDN1, CCL5, RORA and HLA-G. Among the asthma genes identified in genome wide association studies, ROBO1, RORA, HLA-DQB1, IL2RB and PDE10A were differentially expressed during human lung development. CONCLUSIONS: Our data provide insight about the role of asthma susceptibility genes during lung development and suggest common mechanisms underlying lung morphogenesis and pathogenesis of respiratory diseases. |
format | Online Article Text |
id | pubmed-3141421 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-31414212011-07-23 Expression analysis of asthma candidate genes during human and murine lung development Melén, Erik Kho, Alvin T Sharma, Sunita Gaedigk, Roger Leeder, J Steven Mariani, Thomas J Carey, Vincent J Weiss, Scott T Tantisira, Kelan G Respir Res Research BACKGROUND: Little is known about the role of most asthma susceptibility genes during human lung development. Genetic determinants for normal lung development are not only important early in life, but also for later lung function. OBJECTIVE: To investigate the role of expression patterns of well-defined asthma susceptibility genes during human and murine lung development. We hypothesized that genes influencing normal airways development would be over-represented by genes associated with asthma. METHODS: Asthma genes were first identified via comprehensive search of the current literature. Next, we analyzed their expression patterns in the developing human lung during the pseudoglandular (gestational age, 7-16 weeks) and canalicular (17-26 weeks) stages of development, and in the complete developing lung time series of 3 mouse strains: A/J, SW, C57BL6. RESULTS: In total, 96 genes with association to asthma in at least two human populations were identified in the literature. Overall, there was no significant over-representation of the asthma genes among genes differentially expressed during lung development, although trends were seen in the human (Odds ratio, OR 1.22, confidence interval, CI 0.90-1.62) and C57BL6 mouse (OR 1.41, CI 0.92-2.11) data. However, differential expression of some asthma genes was consistent in both developing human and murine lung, e.g. NOD1, EDN1, CCL5, RORA and HLA-G. Among the asthma genes identified in genome wide association studies, ROBO1, RORA, HLA-DQB1, IL2RB and PDE10A were differentially expressed during human lung development. CONCLUSIONS: Our data provide insight about the role of asthma susceptibility genes during lung development and suggest common mechanisms underlying lung morphogenesis and pathogenesis of respiratory diseases. BioMed Central 2011 2011-06-23 /pmc/articles/PMC3141421/ /pubmed/21699702 http://dx.doi.org/10.1186/1465-9921-12-86 Text en Copyright ©2011 Melén et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Melén, Erik Kho, Alvin T Sharma, Sunita Gaedigk, Roger Leeder, J Steven Mariani, Thomas J Carey, Vincent J Weiss, Scott T Tantisira, Kelan G Expression analysis of asthma candidate genes during human and murine lung development |
title | Expression analysis of asthma candidate genes during human and murine lung development |
title_full | Expression analysis of asthma candidate genes during human and murine lung development |
title_fullStr | Expression analysis of asthma candidate genes during human and murine lung development |
title_full_unstemmed | Expression analysis of asthma candidate genes during human and murine lung development |
title_short | Expression analysis of asthma candidate genes during human and murine lung development |
title_sort | expression analysis of asthma candidate genes during human and murine lung development |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3141421/ https://www.ncbi.nlm.nih.gov/pubmed/21699702 http://dx.doi.org/10.1186/1465-9921-12-86 |
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