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Racial differences in the responses to shear stress in human umbilical vein endothelial cells
BACKGROUND: African American ethnicity is an independent risk factor for exaggerated oxidative stress, which is related to inflammation, hypertension, and cardiovascular disease. Recently, we reported that in vitro oxidative stress and inflammation levels differ between African American and Caucasia...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Dove Medical Press
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3141915/ https://www.ncbi.nlm.nih.gov/pubmed/21796257 http://dx.doi.org/10.2147/VHRM.S22435 |
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author | Feairheller, Deborah L Park, Joon-Young Rizzo, Victor Kim, Boa Brown, Michael D |
author_facet | Feairheller, Deborah L Park, Joon-Young Rizzo, Victor Kim, Boa Brown, Michael D |
author_sort | Feairheller, Deborah L |
collection | PubMed |
description | BACKGROUND: African American ethnicity is an independent risk factor for exaggerated oxidative stress, which is related to inflammation, hypertension, and cardiovascular disease. Recently, we reported that in vitro oxidative stress and inflammation levels differ between African American and Caucasian human umbilical vein endothelial cells (HUVECs), African American HUVECs having higher levels of both. However, it remains to be shown whether the cells would respond differently to external stimuli. METHODS: We used a cone and plate viscometer to apply laminar shear stress (LSS) as an aerobic exercise mimetic to compare the responses by race. HUVECs were exposed to static conditions (no LSS), low LSS (5 dyne/cm(2)), and moderate LSS (20 dyne/cm(2)). RESULTS: It was found that African American HUVECs had higher levels of oxidative stress under static conditions, and when LSS was applied protein expression levels (NADPH oxidase NOX2, NOX4 and p47phox subunits, eNOS, SOD2, and catalase) and biomarkers (NO, SOD, and total antioxidant capacity) were modulated to similar levels between race. CONCLUSION: African American HUVECs may be more responsive to LSS stimulus indicating that aerobic exercise prescriptions may be valuable for this population since the potential exists for large in vivo improvements in oxidative stress levels along the endothelial layer in response to increased shear flow. |
format | Online Article Text |
id | pubmed-3141915 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | Dove Medical Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-31419152011-07-27 Racial differences in the responses to shear stress in human umbilical vein endothelial cells Feairheller, Deborah L Park, Joon-Young Rizzo, Victor Kim, Boa Brown, Michael D Vasc Health Risk Manag Original Research BACKGROUND: African American ethnicity is an independent risk factor for exaggerated oxidative stress, which is related to inflammation, hypertension, and cardiovascular disease. Recently, we reported that in vitro oxidative stress and inflammation levels differ between African American and Caucasian human umbilical vein endothelial cells (HUVECs), African American HUVECs having higher levels of both. However, it remains to be shown whether the cells would respond differently to external stimuli. METHODS: We used a cone and plate viscometer to apply laminar shear stress (LSS) as an aerobic exercise mimetic to compare the responses by race. HUVECs were exposed to static conditions (no LSS), low LSS (5 dyne/cm(2)), and moderate LSS (20 dyne/cm(2)). RESULTS: It was found that African American HUVECs had higher levels of oxidative stress under static conditions, and when LSS was applied protein expression levels (NADPH oxidase NOX2, NOX4 and p47phox subunits, eNOS, SOD2, and catalase) and biomarkers (NO, SOD, and total antioxidant capacity) were modulated to similar levels between race. CONCLUSION: African American HUVECs may be more responsive to LSS stimulus indicating that aerobic exercise prescriptions may be valuable for this population since the potential exists for large in vivo improvements in oxidative stress levels along the endothelial layer in response to increased shear flow. Dove Medical Press 2011 2011-07-11 /pmc/articles/PMC3141915/ /pubmed/21796257 http://dx.doi.org/10.2147/VHRM.S22435 Text en © 2011 Feairheller et al, publisher and licensee Dove Medical Press Ltd. This is an Open Access article which permits unrestricted noncommercial use, provided the original work is properly cited. |
spellingShingle | Original Research Feairheller, Deborah L Park, Joon-Young Rizzo, Victor Kim, Boa Brown, Michael D Racial differences in the responses to shear stress in human umbilical vein endothelial cells |
title | Racial differences in the responses to shear stress in human umbilical vein endothelial cells |
title_full | Racial differences in the responses to shear stress in human umbilical vein endothelial cells |
title_fullStr | Racial differences in the responses to shear stress in human umbilical vein endothelial cells |
title_full_unstemmed | Racial differences in the responses to shear stress in human umbilical vein endothelial cells |
title_short | Racial differences in the responses to shear stress in human umbilical vein endothelial cells |
title_sort | racial differences in the responses to shear stress in human umbilical vein endothelial cells |
topic | Original Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3141915/ https://www.ncbi.nlm.nih.gov/pubmed/21796257 http://dx.doi.org/10.2147/VHRM.S22435 |
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