Cargando…

Force Measurements of TCR/pMHC Recognition at T Cell Surface

The rupture forces and adhesion frequencies of single recognition complexes between an affinity selected peptide/MHC complex and a TCR at a murine hybridoma surface were measured using Atomic Force Microscopy. When the CD8 coreceptor is absent, the adhesion frequency depends on the nature of the pep...

Descripción completa

Detalles Bibliográficos
Autores principales: Puech, Pierre-Henri, Nevoltris, Damien, Robert, Philippe, Limozin, Laurent, Boyer, Claude, Bongrand, Pierre
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3142151/
https://www.ncbi.nlm.nih.gov/pubmed/21799834
http://dx.doi.org/10.1371/journal.pone.0022344
_version_ 1782208802991374336
author Puech, Pierre-Henri
Nevoltris, Damien
Robert, Philippe
Limozin, Laurent
Boyer, Claude
Bongrand, Pierre
author_facet Puech, Pierre-Henri
Nevoltris, Damien
Robert, Philippe
Limozin, Laurent
Boyer, Claude
Bongrand, Pierre
author_sort Puech, Pierre-Henri
collection PubMed
description The rupture forces and adhesion frequencies of single recognition complexes between an affinity selected peptide/MHC complex and a TCR at a murine hybridoma surface were measured using Atomic Force Microscopy. When the CD8 coreceptor is absent, the adhesion frequency depends on the nature of the peptide but the rupture force does not. When CD8 is present, no effect of the nature of the peptide is observed. CD8 is proposed to act as a time and distance lock, enabling the shorter TCR molecule to bridge the pMHC and have time to finely read the peptide. Ultimately, such experiments could help the dissection of the sequential steps by which the TCR reads the peptide/MHC complex in order to control T cell activation.
format Online
Article
Text
id pubmed-3142151
institution National Center for Biotechnology Information
language English
publishDate 2011
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-31421512011-07-28 Force Measurements of TCR/pMHC Recognition at T Cell Surface Puech, Pierre-Henri Nevoltris, Damien Robert, Philippe Limozin, Laurent Boyer, Claude Bongrand, Pierre PLoS One Research Article The rupture forces and adhesion frequencies of single recognition complexes between an affinity selected peptide/MHC complex and a TCR at a murine hybridoma surface were measured using Atomic Force Microscopy. When the CD8 coreceptor is absent, the adhesion frequency depends on the nature of the peptide but the rupture force does not. When CD8 is present, no effect of the nature of the peptide is observed. CD8 is proposed to act as a time and distance lock, enabling the shorter TCR molecule to bridge the pMHC and have time to finely read the peptide. Ultimately, such experiments could help the dissection of the sequential steps by which the TCR reads the peptide/MHC complex in order to control T cell activation. Public Library of Science 2011-07-22 /pmc/articles/PMC3142151/ /pubmed/21799834 http://dx.doi.org/10.1371/journal.pone.0022344 Text en Puech et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Puech, Pierre-Henri
Nevoltris, Damien
Robert, Philippe
Limozin, Laurent
Boyer, Claude
Bongrand, Pierre
Force Measurements of TCR/pMHC Recognition at T Cell Surface
title Force Measurements of TCR/pMHC Recognition at T Cell Surface
title_full Force Measurements of TCR/pMHC Recognition at T Cell Surface
title_fullStr Force Measurements of TCR/pMHC Recognition at T Cell Surface
title_full_unstemmed Force Measurements of TCR/pMHC Recognition at T Cell Surface
title_short Force Measurements of TCR/pMHC Recognition at T Cell Surface
title_sort force measurements of tcr/pmhc recognition at t cell surface
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3142151/
https://www.ncbi.nlm.nih.gov/pubmed/21799834
http://dx.doi.org/10.1371/journal.pone.0022344
work_keys_str_mv AT puechpierrehenri forcemeasurementsoftcrpmhcrecognitionattcellsurface
AT nevoltrisdamien forcemeasurementsoftcrpmhcrecognitionattcellsurface
AT robertphilippe forcemeasurementsoftcrpmhcrecognitionattcellsurface
AT limozinlaurent forcemeasurementsoftcrpmhcrecognitionattcellsurface
AT boyerclaude forcemeasurementsoftcrpmhcrecognitionattcellsurface
AT bongrandpierre forcemeasurementsoftcrpmhcrecognitionattcellsurface