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The Histological and Immunohistochemical Aspects of Bile Reflux in Patients with Gastroesophageal Reflux Disease

Introduction. The pathogenesis of GERD is strongly related with mixed acid and bile reflux. Benign and malignant esophageal and gastric lesions have been associated with synergetic activity between those parameters. Bile reflux causes reactive gastropathy evaluated with Bile Reflux Index (BRI). The...

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Autores principales: Nakos, Andreas, Kouklakis, Georgios, Pitiakoudis, Michail, Zezos, Petros, Efraimidou, Eleni, Giatromanolaki, Alexandra, Polychronidis, Alexandros, Liratzopoulos, Nikolaos, Sivridis, Efthimios, Simopoulos, Konstantinos
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3142670/
https://www.ncbi.nlm.nih.gov/pubmed/21822428
http://dx.doi.org/10.1155/2011/905872
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author Nakos, Andreas
Kouklakis, Georgios
Pitiakoudis, Michail
Zezos, Petros
Efraimidou, Eleni
Giatromanolaki, Alexandra
Polychronidis, Alexandros
Liratzopoulos, Nikolaos
Sivridis, Efthimios
Simopoulos, Konstantinos
author_facet Nakos, Andreas
Kouklakis, Georgios
Pitiakoudis, Michail
Zezos, Petros
Efraimidou, Eleni
Giatromanolaki, Alexandra
Polychronidis, Alexandros
Liratzopoulos, Nikolaos
Sivridis, Efthimios
Simopoulos, Konstantinos
author_sort Nakos, Andreas
collection PubMed
description Introduction. The pathogenesis of GERD is strongly related with mixed acid and bile reflux. Benign and malignant esophageal and gastric lesions have been associated with synergetic activity between those parameters. Bile reflux causes reactive gastropathy evaluated with Bile Reflux Index (BRI). The aim was to investigate if the sequence: bile reflux-intestinal metaplasia-GERD-esophagitis, is associated with apoptotic/oncogenetic disturbances. Materials/Methods. Fifteen asymptomatic subjects and 53 GERD patients underwent gastroscopy with biopsies. The specimens examined histologically and immunohistochemically for p53, Ki-67, Bax, and Bcl-2. Results. Elevated BRI score detected in 47% (25/53) of patients with GERD and in 13% (2/15) of controls (P = 0.02). Severe esophageal lesions were significantly more common in BRI (+) patients (14/25) compared to BRI (−) ones (P = 0.0049). Immunohistochemical analysis did not show associations between BRI score and biomarker expression. Conclusions. Bile reflux gastropathy is associated with GERD severity, but not with oncogene expression or apoptotic discrepancies of the upper GI mucosa.
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spelling pubmed-31426702011-08-05 The Histological and Immunohistochemical Aspects of Bile Reflux in Patients with Gastroesophageal Reflux Disease Nakos, Andreas Kouklakis, Georgios Pitiakoudis, Michail Zezos, Petros Efraimidou, Eleni Giatromanolaki, Alexandra Polychronidis, Alexandros Liratzopoulos, Nikolaos Sivridis, Efthimios Simopoulos, Konstantinos Gastroenterol Res Pract Clinical Study Introduction. The pathogenesis of GERD is strongly related with mixed acid and bile reflux. Benign and malignant esophageal and gastric lesions have been associated with synergetic activity between those parameters. Bile reflux causes reactive gastropathy evaluated with Bile Reflux Index (BRI). The aim was to investigate if the sequence: bile reflux-intestinal metaplasia-GERD-esophagitis, is associated with apoptotic/oncogenetic disturbances. Materials/Methods. Fifteen asymptomatic subjects and 53 GERD patients underwent gastroscopy with biopsies. The specimens examined histologically and immunohistochemically for p53, Ki-67, Bax, and Bcl-2. Results. Elevated BRI score detected in 47% (25/53) of patients with GERD and in 13% (2/15) of controls (P = 0.02). Severe esophageal lesions were significantly more common in BRI (+) patients (14/25) compared to BRI (−) ones (P = 0.0049). Immunohistochemical analysis did not show associations between BRI score and biomarker expression. Conclusions. Bile reflux gastropathy is associated with GERD severity, but not with oncogene expression or apoptotic discrepancies of the upper GI mucosa. Hindawi Publishing Corporation 2011 2011-07-24 /pmc/articles/PMC3142670/ /pubmed/21822428 http://dx.doi.org/10.1155/2011/905872 Text en Copyright © 2011 Andreas Nakos et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Clinical Study
Nakos, Andreas
Kouklakis, Georgios
Pitiakoudis, Michail
Zezos, Petros
Efraimidou, Eleni
Giatromanolaki, Alexandra
Polychronidis, Alexandros
Liratzopoulos, Nikolaos
Sivridis, Efthimios
Simopoulos, Konstantinos
The Histological and Immunohistochemical Aspects of Bile Reflux in Patients with Gastroesophageal Reflux Disease
title The Histological and Immunohistochemical Aspects of Bile Reflux in Patients with Gastroesophageal Reflux Disease
title_full The Histological and Immunohistochemical Aspects of Bile Reflux in Patients with Gastroesophageal Reflux Disease
title_fullStr The Histological and Immunohistochemical Aspects of Bile Reflux in Patients with Gastroesophageal Reflux Disease
title_full_unstemmed The Histological and Immunohistochemical Aspects of Bile Reflux in Patients with Gastroesophageal Reflux Disease
title_short The Histological and Immunohistochemical Aspects of Bile Reflux in Patients with Gastroesophageal Reflux Disease
title_sort histological and immunohistochemical aspects of bile reflux in patients with gastroesophageal reflux disease
topic Clinical Study
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3142670/
https://www.ncbi.nlm.nih.gov/pubmed/21822428
http://dx.doi.org/10.1155/2011/905872
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