Direct Spectrophotometric Assay for Benzaldehyde Lyase Activity

Benzaldehyde lyase from Pseudomonas fluorescens Biovar I. (BAL, EC 4.1.2.38) is a versatile catalyst for the organic synthesis of chiral α-hydroxy ketones. To allow fast assessment of enzyme activity, a direct spectrophotometric assay is desirable. Here, a new robust and easy-to-handle assay based o...

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Detalles Bibliográficos
Autores principales: Natalia, Dessy, Kohlmann, Christina, Ansorge-Schumacher, Marion B., Greiner, Lasse
Formato: Online Artículo Texto
Lenguaje:English
Publicado: SAGE-Hindawi Access to Research 2011
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3142707/
https://www.ncbi.nlm.nih.gov/pubmed/21804944
http://dx.doi.org/10.4061/2011/478925
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author Natalia, Dessy
Kohlmann, Christina
Ansorge-Schumacher, Marion B.
Greiner, Lasse
author_facet Natalia, Dessy
Kohlmann, Christina
Ansorge-Schumacher, Marion B.
Greiner, Lasse
author_sort Natalia, Dessy
collection PubMed
description Benzaldehyde lyase from Pseudomonas fluorescens Biovar I. (BAL, EC 4.1.2.38) is a versatile catalyst for the organic synthesis of chiral α-hydroxy ketones. To allow fast assessment of enzyme activity, a direct spectrophotometric assay is desirable. Here, a new robust and easy-to-handle assay based on UV absorption is presented. The assay developed is based on the ligation of the α-hydroxy ketone (R)-2,2′-furoin from 2-furaldehyde. A robust assay with direct monitoring of the product is facilitated with a convenient concentration working range minimising experimental associated with low concentrations.
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spelling pubmed-31427072011-07-29 Direct Spectrophotometric Assay for Benzaldehyde Lyase Activity Natalia, Dessy Kohlmann, Christina Ansorge-Schumacher, Marion B. Greiner, Lasse Biotechnol Res Int Research Article Benzaldehyde lyase from Pseudomonas fluorescens Biovar I. (BAL, EC 4.1.2.38) is a versatile catalyst for the organic synthesis of chiral α-hydroxy ketones. To allow fast assessment of enzyme activity, a direct spectrophotometric assay is desirable. Here, a new robust and easy-to-handle assay based on UV absorption is presented. The assay developed is based on the ligation of the α-hydroxy ketone (R)-2,2′-furoin from 2-furaldehyde. A robust assay with direct monitoring of the product is facilitated with a convenient concentration working range minimising experimental associated with low concentrations. SAGE-Hindawi Access to Research 2011-07-14 /pmc/articles/PMC3142707/ /pubmed/21804944 http://dx.doi.org/10.4061/2011/478925 Text en Copyright © 2011 Dessy Natalia et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Natalia, Dessy
Kohlmann, Christina
Ansorge-Schumacher, Marion B.
Greiner, Lasse
Direct Spectrophotometric Assay for Benzaldehyde Lyase Activity
title Direct Spectrophotometric Assay for Benzaldehyde Lyase Activity
title_full Direct Spectrophotometric Assay for Benzaldehyde Lyase Activity
title_fullStr Direct Spectrophotometric Assay for Benzaldehyde Lyase Activity
title_full_unstemmed Direct Spectrophotometric Assay for Benzaldehyde Lyase Activity
title_short Direct Spectrophotometric Assay for Benzaldehyde Lyase Activity
title_sort direct spectrophotometric assay for benzaldehyde lyase activity
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3142707/
https://www.ncbi.nlm.nih.gov/pubmed/21804944
http://dx.doi.org/10.4061/2011/478925
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AT ansorgeschumachermarionb directspectrophotometricassayforbenzaldehydelyaseactivity
AT greinerlasse directspectrophotometricassayforbenzaldehydelyaseactivity

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