Glioblastoma Stem-Like Cells—Biology and Therapeutic Implications

The cancer stem-cell hypothesis proposes that malignant tumors are likely to encompass a cellular hierarchy that parallels normal tissue and may be responsible for the maintenance and recurrence of glioblastoma multiforme (GBM) in patients. The purpose of this manuscript is to review methods for opt...

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Detalles Bibliográficos
Autores principales: Gürsel, Demirkan B., Shin, Benjamin J., Burkhardt, Jan-Karl, Kesavabhotla, Kartik, Schlaff, Cody D., Boockvar, John A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Molecular Diversity Preservation International (MDPI) 2011
Materias:
Review
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3142771/
https://www.ncbi.nlm.nih.gov/pubmed/21796273
http://dx.doi.org/10.3390/cancers3022655
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author Gürsel, Demirkan B.
Shin, Benjamin J.
Burkhardt, Jan-Karl
Kesavabhotla, Kartik
Schlaff, Cody D.
Boockvar, John A.
author_facet Gürsel, Demirkan B.
Shin, Benjamin J.
Burkhardt, Jan-Karl
Kesavabhotla, Kartik
Schlaff, Cody D.
Boockvar, John A.
author_sort Gürsel, Demirkan B.
collection PubMed
description The cancer stem-cell hypothesis proposes that malignant tumors are likely to encompass a cellular hierarchy that parallels normal tissue and may be responsible for the maintenance and recurrence of glioblastoma multiforme (GBM) in patients. The purpose of this manuscript is to review methods for optimizing the derivation and culturing of stem-like cells also known as tumor stem cells (TSCs) from patient-derived GBM tissue samples. The hallmarks of TSCs are that they must be able to self-renew and retain tumorigenicity. The isolation, optimization and derivation of TSCs as outlined in this review, will be important in understanding biology and therapeutic applications related to these cells.
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spelling pubmed-31427712011-07-25 Glioblastoma Stem-Like Cells—Biology and Therapeutic Implications Gürsel, Demirkan B. Shin, Benjamin J. Burkhardt, Jan-Karl Kesavabhotla, Kartik Schlaff, Cody D. Boockvar, John A. Cancers (Basel) Review The cancer stem-cell hypothesis proposes that malignant tumors are likely to encompass a cellular hierarchy that parallels normal tissue and may be responsible for the maintenance and recurrence of glioblastoma multiforme (GBM) in patients. The purpose of this manuscript is to review methods for optimizing the derivation and culturing of stem-like cells also known as tumor stem cells (TSCs) from patient-derived GBM tissue samples. The hallmarks of TSCs are that they must be able to self-renew and retain tumorigenicity. The isolation, optimization and derivation of TSCs as outlined in this review, will be important in understanding biology and therapeutic applications related to these cells. Molecular Diversity Preservation International (MDPI) 2011-06-10 /pmc/articles/PMC3142771/ /pubmed/21796273 http://dx.doi.org/10.3390/cancers3022655 Text en © 2011 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Review
Gürsel, Demirkan B.
Shin, Benjamin J.
Burkhardt, Jan-Karl
Kesavabhotla, Kartik
Schlaff, Cody D.
Boockvar, John A.
Glioblastoma Stem-Like Cells—Biology and Therapeutic Implications
title Glioblastoma Stem-Like Cells—Biology and Therapeutic Implications
title_full Glioblastoma Stem-Like Cells—Biology and Therapeutic Implications
title_fullStr Glioblastoma Stem-Like Cells—Biology and Therapeutic Implications
title_full_unstemmed Glioblastoma Stem-Like Cells—Biology and Therapeutic Implications
title_short Glioblastoma Stem-Like Cells—Biology and Therapeutic Implications
title_sort glioblastoma stem-like cells—biology and therapeutic implications
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3142771/
https://www.ncbi.nlm.nih.gov/pubmed/21796273
http://dx.doi.org/10.3390/cancers3022655
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