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Colonization of Phlebotomus papatasi changes the effect of pre-immunization with saliva from lack of protection towards protection against experimental challenge with Leishmania major and saliva

BACKGROUND: Sand fly saliva has been postulated as a potential vaccine or as a vaccine component within multi component vaccine against leishmaniasis. It is important to note that these studies were performed using long-term colonized Phlebotomus papatasi. The effect of sand flies colonization on th...

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Autores principales: Ben Hadj Ahmed, Sami, Kaabi, Belhassen, Chelbi, Ifhem, Cherni, Saifeddine, Derbali, Mohamed, Laouini, Dhafer, Zhioua, Elyes
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3143093/
https://www.ncbi.nlm.nih.gov/pubmed/21726438
http://dx.doi.org/10.1186/1756-3305-4-126
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author Ben Hadj Ahmed, Sami
Kaabi, Belhassen
Chelbi, Ifhem
Cherni, Saifeddine
Derbali, Mohamed
Laouini, Dhafer
Zhioua, Elyes
author_facet Ben Hadj Ahmed, Sami
Kaabi, Belhassen
Chelbi, Ifhem
Cherni, Saifeddine
Derbali, Mohamed
Laouini, Dhafer
Zhioua, Elyes
author_sort Ben Hadj Ahmed, Sami
collection PubMed
description BACKGROUND: Sand fly saliva has been postulated as a potential vaccine or as a vaccine component within multi component vaccine against leishmaniasis. It is important to note that these studies were performed using long-term colonized Phlebotomus papatasi. The effect of sand flies colonization on the outcome of Leishmania infection is reported. RESULTS: While pre-immunization of mice with salivary gland homogenate (SGH) of long-term colonized (F5 and beyond) female Phlebotomus papatasi induced protection against Leishmania major co-inoculated with the same type of SGH, pre-immunization of mice with SGH of recently colonized (F2 and F3) female P. papatasi did not confer protection against L. major co-inoculated with the same type of SGH. Our data showed for the first time that a shift from lack of protection to protection occurs at the fourth generation (F4) during the colonization process of P. papatasi. CONCLUSION: For the development of a sand fly saliva-based vaccine, inferences based on long-term colonized populations of sand flies should be treated with caution as colonization of P. papatasi appears to modulate the outcome of L. major infection from lack of protection to protection.
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spelling pubmed-31430932011-07-26 Colonization of Phlebotomus papatasi changes the effect of pre-immunization with saliva from lack of protection towards protection against experimental challenge with Leishmania major and saliva Ben Hadj Ahmed, Sami Kaabi, Belhassen Chelbi, Ifhem Cherni, Saifeddine Derbali, Mohamed Laouini, Dhafer Zhioua, Elyes Parasit Vectors Research BACKGROUND: Sand fly saliva has been postulated as a potential vaccine or as a vaccine component within multi component vaccine against leishmaniasis. It is important to note that these studies were performed using long-term colonized Phlebotomus papatasi. The effect of sand flies colonization on the outcome of Leishmania infection is reported. RESULTS: While pre-immunization of mice with salivary gland homogenate (SGH) of long-term colonized (F5 and beyond) female Phlebotomus papatasi induced protection against Leishmania major co-inoculated with the same type of SGH, pre-immunization of mice with SGH of recently colonized (F2 and F3) female P. papatasi did not confer protection against L. major co-inoculated with the same type of SGH. Our data showed for the first time that a shift from lack of protection to protection occurs at the fourth generation (F4) during the colonization process of P. papatasi. CONCLUSION: For the development of a sand fly saliva-based vaccine, inferences based on long-term colonized populations of sand flies should be treated with caution as colonization of P. papatasi appears to modulate the outcome of L. major infection from lack of protection to protection. BioMed Central 2011-07-04 /pmc/articles/PMC3143093/ /pubmed/21726438 http://dx.doi.org/10.1186/1756-3305-4-126 Text en Copyright ©2011 Ben Hadj Ahmed et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Ben Hadj Ahmed, Sami
Kaabi, Belhassen
Chelbi, Ifhem
Cherni, Saifeddine
Derbali, Mohamed
Laouini, Dhafer
Zhioua, Elyes
Colonization of Phlebotomus papatasi changes the effect of pre-immunization with saliva from lack of protection towards protection against experimental challenge with Leishmania major and saliva
title Colonization of Phlebotomus papatasi changes the effect of pre-immunization with saliva from lack of protection towards protection against experimental challenge with Leishmania major and saliva
title_full Colonization of Phlebotomus papatasi changes the effect of pre-immunization with saliva from lack of protection towards protection against experimental challenge with Leishmania major and saliva
title_fullStr Colonization of Phlebotomus papatasi changes the effect of pre-immunization with saliva from lack of protection towards protection against experimental challenge with Leishmania major and saliva
title_full_unstemmed Colonization of Phlebotomus papatasi changes the effect of pre-immunization with saliva from lack of protection towards protection against experimental challenge with Leishmania major and saliva
title_short Colonization of Phlebotomus papatasi changes the effect of pre-immunization with saliva from lack of protection towards protection against experimental challenge with Leishmania major and saliva
title_sort colonization of phlebotomus papatasi changes the effect of pre-immunization with saliva from lack of protection towards protection against experimental challenge with leishmania major and saliva
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3143093/
https://www.ncbi.nlm.nih.gov/pubmed/21726438
http://dx.doi.org/10.1186/1756-3305-4-126
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