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Quantitative determination, by real-time reverse transcription polymerase chain reaction, of aromatase mRNA in invasive ductal carcinoma of the breast

BACKGROUND: Estrogen is a mitogenic factor that is implicated in the genesis and progression of breast cancer via its binding to estrogen receptor (ER)-α. Synthesis of estrogen in situ is believed to be catalyzed mainly by aromatase. Previous studies comparing the relative contributions from tumor c...

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Autores principales: Zhang, Zhenhuan, Yamashita, Hiroko, Toyama, Tatsuya, Omoto, Yoko, Sugiura, Hiroshi, Hara, Yasuo, Wu, Xueqing, Kobayashi, Shunzo, Iwase, Hirotaka
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2003
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC314416/
https://www.ncbi.nlm.nih.gov/pubmed/14580261
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author Zhang, Zhenhuan
Yamashita, Hiroko
Toyama, Tatsuya
Omoto, Yoko
Sugiura, Hiroshi
Hara, Yasuo
Wu, Xueqing
Kobayashi, Shunzo
Iwase, Hirotaka
author_facet Zhang, Zhenhuan
Yamashita, Hiroko
Toyama, Tatsuya
Omoto, Yoko
Sugiura, Hiroshi
Hara, Yasuo
Wu, Xueqing
Kobayashi, Shunzo
Iwase, Hirotaka
author_sort Zhang, Zhenhuan
collection PubMed
description BACKGROUND: Estrogen is a mitogenic factor that is implicated in the genesis and progression of breast cancer via its binding to estrogen receptor (ER)-α. Synthesis of estrogen in situ is believed to be catalyzed mainly by aromatase. Previous studies comparing the relative contributions from tumor cells and stromal cells to local estrogen synthesis, as assessed by immunohistochemical analysis, were quite controversial and no consistent relationship was found between the presence of aromatase and any clinicopathologic factor. In addition, previous studies into aromatase gene expression and clinicopathologic factors are limited. METHODS: We assessed the level of expression of aromatase mRNA, using quantitative real-time RT-PCR, in 162 cases of invasive ductal carcinoma of the breast. Associations between aromatase expression and different clinicopathologic factors were sought. RESULTS: It was found that aromatase mRNA was expressed at significantly higher levels in patients older than 50 years, in those without axillary lymph node involvement, in those with tumor size less than 2 cm, and in ER-α positive tumors. However, no relationship was found between aromatase mRNA expression and any other clinicopathologic factor, including histologic grade and progesterone receptor status. Patients with high levels of expression of aromatase mRNA tended to have a better prognosis than did those patients with low expression. CONCLUSION: These findings imply that ER-α and aromatase may be coexpressed in endocrine responsive patients. They may also indicate that aromatase expression could be a marker of endocrine responsiveness, and it may have prognostic implications for breast cancer progression.
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spelling pubmed-3144162004-01-17 Quantitative determination, by real-time reverse transcription polymerase chain reaction, of aromatase mRNA in invasive ductal carcinoma of the breast Zhang, Zhenhuan Yamashita, Hiroko Toyama, Tatsuya Omoto, Yoko Sugiura, Hiroshi Hara, Yasuo Wu, Xueqing Kobayashi, Shunzo Iwase, Hirotaka Breast Cancer Res Research Article BACKGROUND: Estrogen is a mitogenic factor that is implicated in the genesis and progression of breast cancer via its binding to estrogen receptor (ER)-α. Synthesis of estrogen in situ is believed to be catalyzed mainly by aromatase. Previous studies comparing the relative contributions from tumor cells and stromal cells to local estrogen synthesis, as assessed by immunohistochemical analysis, were quite controversial and no consistent relationship was found between the presence of aromatase and any clinicopathologic factor. In addition, previous studies into aromatase gene expression and clinicopathologic factors are limited. METHODS: We assessed the level of expression of aromatase mRNA, using quantitative real-time RT-PCR, in 162 cases of invasive ductal carcinoma of the breast. Associations between aromatase expression and different clinicopathologic factors were sought. RESULTS: It was found that aromatase mRNA was expressed at significantly higher levels in patients older than 50 years, in those without axillary lymph node involvement, in those with tumor size less than 2 cm, and in ER-α positive tumors. However, no relationship was found between aromatase mRNA expression and any other clinicopathologic factor, including histologic grade and progesterone receptor status. Patients with high levels of expression of aromatase mRNA tended to have a better prognosis than did those patients with low expression. CONCLUSION: These findings imply that ER-α and aromatase may be coexpressed in endocrine responsive patients. They may also indicate that aromatase expression could be a marker of endocrine responsiveness, and it may have prognostic implications for breast cancer progression. BioMed Central 2003 2003-10-09 /pmc/articles/PMC314416/ /pubmed/14580261 Text en Copyright © 2003 Zhang et al., licensee BioMed Central Ltd. This is an Open Access article: verbatim copying and redistribution of this article are permitted in all media for any purpose, provided this notice is preserved along with the article's original URL.
spellingShingle Research Article
Zhang, Zhenhuan
Yamashita, Hiroko
Toyama, Tatsuya
Omoto, Yoko
Sugiura, Hiroshi
Hara, Yasuo
Wu, Xueqing
Kobayashi, Shunzo
Iwase, Hirotaka
Quantitative determination, by real-time reverse transcription polymerase chain reaction, of aromatase mRNA in invasive ductal carcinoma of the breast
title Quantitative determination, by real-time reverse transcription polymerase chain reaction, of aromatase mRNA in invasive ductal carcinoma of the breast
title_full Quantitative determination, by real-time reverse transcription polymerase chain reaction, of aromatase mRNA in invasive ductal carcinoma of the breast
title_fullStr Quantitative determination, by real-time reverse transcription polymerase chain reaction, of aromatase mRNA in invasive ductal carcinoma of the breast
title_full_unstemmed Quantitative determination, by real-time reverse transcription polymerase chain reaction, of aromatase mRNA in invasive ductal carcinoma of the breast
title_short Quantitative determination, by real-time reverse transcription polymerase chain reaction, of aromatase mRNA in invasive ductal carcinoma of the breast
title_sort quantitative determination, by real-time reverse transcription polymerase chain reaction, of aromatase mrna in invasive ductal carcinoma of the breast
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC314416/
https://www.ncbi.nlm.nih.gov/pubmed/14580261
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