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Expression of Transposable Elements in Neural Tissues during Xenopus Development

Transposable elements comprise a large proportion of animal genomes. Transposons can have detrimental effects on genome stability but also offer positive roles for genome evolution and gene expression regulation. Proper balance of the positive and deleterious effects of transposons is crucial for ce...

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Autores principales: Faunes, Fernando, Sanchez, Natalia, Moreno, Mauricio, Olivares, Gonzalo H., Lee-Liu, Dasfne, Almonacid, Leonardo, Slater, Alex W., Norambuena, Tomas, Taft, Ryan J., Mattick, John S., Melo, Francisco, Larrain, Juan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3144230/
https://www.ncbi.nlm.nih.gov/pubmed/21818339
http://dx.doi.org/10.1371/journal.pone.0022569
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author Faunes, Fernando
Sanchez, Natalia
Moreno, Mauricio
Olivares, Gonzalo H.
Lee-Liu, Dasfne
Almonacid, Leonardo
Slater, Alex W.
Norambuena, Tomas
Taft, Ryan J.
Mattick, John S.
Melo, Francisco
Larrain, Juan
author_facet Faunes, Fernando
Sanchez, Natalia
Moreno, Mauricio
Olivares, Gonzalo H.
Lee-Liu, Dasfne
Almonacid, Leonardo
Slater, Alex W.
Norambuena, Tomas
Taft, Ryan J.
Mattick, John S.
Melo, Francisco
Larrain, Juan
author_sort Faunes, Fernando
collection PubMed
description Transposable elements comprise a large proportion of animal genomes. Transposons can have detrimental effects on genome stability but also offer positive roles for genome evolution and gene expression regulation. Proper balance of the positive and deleterious effects of transposons is crucial for cell homeostasis and requires a mechanism that tightly regulates their expression. Herein we describe the expression of DNA transposons of the Tc1/mariner superfamily during Xenopus development. Sense and antisense transcripts containing complete Tc1-2_Xt were detected in Xenopus embryos. Both transcripts were found in zygotic stages and were mainly localized in Spemann's organizer and neural tissues. In addition, the Tc1-like elements Eagle, Froggy, Jumpy, Maya, Xeminos and TXr were also expressed in zygotic stages but not oocytes in X. tropicalis. Interestingly, although Tc1-2_Xt transcripts were not detected in Xenopus laevis embryos, transcripts from other two Tc1-like elements (TXr and TXz) presented a similar temporal and spatial pattern during X. laevis development. Deep sequencing analysis of Xenopus tropicalis gastrulae showed that PIWI-interacting RNAs (piRNAs) are specifically derived from several Tc1-like elements. The localized expression of Tc1-like elements in neural tissues suggests that they could play a role during the development of the Xenopus nervous system.
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spelling pubmed-31442302011-08-04 Expression of Transposable Elements in Neural Tissues during Xenopus Development Faunes, Fernando Sanchez, Natalia Moreno, Mauricio Olivares, Gonzalo H. Lee-Liu, Dasfne Almonacid, Leonardo Slater, Alex W. Norambuena, Tomas Taft, Ryan J. Mattick, John S. Melo, Francisco Larrain, Juan PLoS One Research Article Transposable elements comprise a large proportion of animal genomes. Transposons can have detrimental effects on genome stability but also offer positive roles for genome evolution and gene expression regulation. Proper balance of the positive and deleterious effects of transposons is crucial for cell homeostasis and requires a mechanism that tightly regulates their expression. Herein we describe the expression of DNA transposons of the Tc1/mariner superfamily during Xenopus development. Sense and antisense transcripts containing complete Tc1-2_Xt were detected in Xenopus embryos. Both transcripts were found in zygotic stages and were mainly localized in Spemann's organizer and neural tissues. In addition, the Tc1-like elements Eagle, Froggy, Jumpy, Maya, Xeminos and TXr were also expressed in zygotic stages but not oocytes in X. tropicalis. Interestingly, although Tc1-2_Xt transcripts were not detected in Xenopus laevis embryos, transcripts from other two Tc1-like elements (TXr and TXz) presented a similar temporal and spatial pattern during X. laevis development. Deep sequencing analysis of Xenopus tropicalis gastrulae showed that PIWI-interacting RNAs (piRNAs) are specifically derived from several Tc1-like elements. The localized expression of Tc1-like elements in neural tissues suggests that they could play a role during the development of the Xenopus nervous system. Public Library of Science 2011-07-26 /pmc/articles/PMC3144230/ /pubmed/21818339 http://dx.doi.org/10.1371/journal.pone.0022569 Text en Faunes et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Faunes, Fernando
Sanchez, Natalia
Moreno, Mauricio
Olivares, Gonzalo H.
Lee-Liu, Dasfne
Almonacid, Leonardo
Slater, Alex W.
Norambuena, Tomas
Taft, Ryan J.
Mattick, John S.
Melo, Francisco
Larrain, Juan
Expression of Transposable Elements in Neural Tissues during Xenopus Development
title Expression of Transposable Elements in Neural Tissues during Xenopus Development
title_full Expression of Transposable Elements in Neural Tissues during Xenopus Development
title_fullStr Expression of Transposable Elements in Neural Tissues during Xenopus Development
title_full_unstemmed Expression of Transposable Elements in Neural Tissues during Xenopus Development
title_short Expression of Transposable Elements in Neural Tissues during Xenopus Development
title_sort expression of transposable elements in neural tissues during xenopus development
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3144230/
https://www.ncbi.nlm.nih.gov/pubmed/21818339
http://dx.doi.org/10.1371/journal.pone.0022569
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