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Expression, purification, crystallization and preliminary crystallographic analysis of a putative Clostridium difficile surface protein Cwp19
Cwp19 is a putatively surface-located protein from Clostridium difficile. A recombinant N-terminal protein (residues 27–401) lacking the signal peptide and the C-terminal cell-wall-binding repeats (PFam04122) was crystallized using the sitting-drop vapour-diffusion method and diffracted to 2 Å resol...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
International Union of Crystallography
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3144791/ https://www.ncbi.nlm.nih.gov/pubmed/21795789 http://dx.doi.org/10.1107/S1744309111016770 |
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author | Kirby, Jonathan M. Thiyagarajan, Nethaji Roberts, April K. Shone, Clifford C. Acharya, K. Ravi |
author_facet | Kirby, Jonathan M. Thiyagarajan, Nethaji Roberts, April K. Shone, Clifford C. Acharya, K. Ravi |
author_sort | Kirby, Jonathan M. |
collection | PubMed |
description | Cwp19 is a putatively surface-located protein from Clostridium difficile. A recombinant N-terminal protein (residues 27–401) lacking the signal peptide and the C-terminal cell-wall-binding repeats (PFam04122) was crystallized using the sitting-drop vapour-diffusion method and diffracted to 2 Å resolution. The crystal appeared to belong to the primitive monoclinic space group P2(1), with unit-cell parameters a = 109.1, b = 61.2, c = 109.2 Å, β = 111.85°, and is estimated to contain two molecules of Cwp19 per asymmetric unit. |
format | Online Article Text |
id | pubmed-3144791 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | International Union of Crystallography |
record_format | MEDLINE/PubMed |
spelling | pubmed-31447912011-08-03 Expression, purification, crystallization and preliminary crystallographic analysis of a putative Clostridium difficile surface protein Cwp19 Kirby, Jonathan M. Thiyagarajan, Nethaji Roberts, April K. Shone, Clifford C. Acharya, K. Ravi Acta Crystallogr Sect F Struct Biol Cryst Commun Crystallization Communications Cwp19 is a putatively surface-located protein from Clostridium difficile. A recombinant N-terminal protein (residues 27–401) lacking the signal peptide and the C-terminal cell-wall-binding repeats (PFam04122) was crystallized using the sitting-drop vapour-diffusion method and diffracted to 2 Å resolution. The crystal appeared to belong to the primitive monoclinic space group P2(1), with unit-cell parameters a = 109.1, b = 61.2, c = 109.2 Å, β = 111.85°, and is estimated to contain two molecules of Cwp19 per asymmetric unit. International Union of Crystallography 2011-06-30 /pmc/articles/PMC3144791/ /pubmed/21795789 http://dx.doi.org/10.1107/S1744309111016770 Text en © Kirby et al. 2011 http://creativecommons.org/licenses/by/2.0/uk/ This is an open-access article distributed under the terms of the Creative Commons Attribution Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original authors and source are cited. |
spellingShingle | Crystallization Communications Kirby, Jonathan M. Thiyagarajan, Nethaji Roberts, April K. Shone, Clifford C. Acharya, K. Ravi Expression, purification, crystallization and preliminary crystallographic analysis of a putative Clostridium difficile surface protein Cwp19 |
title | Expression, purification, crystallization and preliminary crystallographic analysis of a putative Clostridium difficile surface protein Cwp19 |
title_full | Expression, purification, crystallization and preliminary crystallographic analysis of a putative Clostridium difficile surface protein Cwp19 |
title_fullStr | Expression, purification, crystallization and preliminary crystallographic analysis of a putative Clostridium difficile surface protein Cwp19 |
title_full_unstemmed | Expression, purification, crystallization and preliminary crystallographic analysis of a putative Clostridium difficile surface protein Cwp19 |
title_short | Expression, purification, crystallization and preliminary crystallographic analysis of a putative Clostridium difficile surface protein Cwp19 |
title_sort | expression, purification, crystallization and preliminary crystallographic analysis of a putative clostridium difficile surface protein cwp19 |
topic | Crystallization Communications |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3144791/ https://www.ncbi.nlm.nih.gov/pubmed/21795789 http://dx.doi.org/10.1107/S1744309111016770 |
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