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Expression, crystallization and preliminary crystallographic study of the C-terminal half of nsp2 from SARS coronavirus
SARS coronavirus (SARS-CoV) is the aetiological agent of the highly infectious severe acute respiratory syndrome (SARS). To gain a better understanding of SARS-CoV replication and transcription proteins, a preliminary X-ray crystallographic study of the C-terminal domain of SARS-CoV nonstructural p...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
International Union of Crystallography
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3144797/ https://www.ncbi.nlm.nih.gov/pubmed/21795795 http://dx.doi.org/10.1107/S1744309111017829 |
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author | Li, Yuanyuan Ren, Zhilin Bao, Zehua Ming, Zhenhua Li, Xuemei |
author_facet | Li, Yuanyuan Ren, Zhilin Bao, Zehua Ming, Zhenhua Li, Xuemei |
author_sort | Li, Yuanyuan |
collection | PubMed |
description | SARS coronavirus (SARS-CoV) is the aetiological agent of the highly infectious severe acute respiratory syndrome (SARS). To gain a better understanding of SARS-CoV replication and transcription proteins, a preliminary X-ray crystallographic study of the C-terminal domain of SARS-CoV nonstructural protein 2 (nsp2) is reported here. The C-terminal domain of SARS-CoV nsp2 was cloned, overexpressed, purified and crystallized using polyethylene glycol 5000 monomethyl ether as the precipitant; the crystals diffracted to 2.5 Å resolution. The crystals belonged to space group P6(5), with unit-cell parameters a = b = 112.8, c = 91.1 Å, α = β = 90, γ = 120°. One molecule is assumed to be present per asymmetric unit, which gives a Matthews coefficient of 2.89 Å(3) Da(−1) and a solvent content of 56.2%. |
format | Online Article Text |
id | pubmed-3144797 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | International Union of Crystallography |
record_format | MEDLINE/PubMed |
spelling | pubmed-31447972013-07-01 Expression, crystallization and preliminary crystallographic study of the C-terminal half of nsp2 from SARS coronavirus Li, Yuanyuan Ren, Zhilin Bao, Zehua Ming, Zhenhua Li, Xuemei Acta Crystallogr Sect F Struct Biol Cryst Commun Crystallization Communications SARS coronavirus (SARS-CoV) is the aetiological agent of the highly infectious severe acute respiratory syndrome (SARS). To gain a better understanding of SARS-CoV replication and transcription proteins, a preliminary X-ray crystallographic study of the C-terminal domain of SARS-CoV nonstructural protein 2 (nsp2) is reported here. The C-terminal domain of SARS-CoV nsp2 was cloned, overexpressed, purified and crystallized using polyethylene glycol 5000 monomethyl ether as the precipitant; the crystals diffracted to 2.5 Å resolution. The crystals belonged to space group P6(5), with unit-cell parameters a = b = 112.8, c = 91.1 Å, α = β = 90, γ = 120°. One molecule is assumed to be present per asymmetric unit, which gives a Matthews coefficient of 2.89 Å(3) Da(−1) and a solvent content of 56.2%. International Union of Crystallography 2011-06-30 /pmc/articles/PMC3144797/ /pubmed/21795795 http://dx.doi.org/10.1107/S1744309111017829 Text en © International Union of Crystallography 2011 |
spellingShingle | Crystallization Communications Li, Yuanyuan Ren, Zhilin Bao, Zehua Ming, Zhenhua Li, Xuemei Expression, crystallization and preliminary crystallographic study of the C-terminal half of nsp2 from SARS coronavirus |
title | Expression, crystallization and preliminary crystallographic study of the C-terminal half of nsp2 from SARS coronavirus |
title_full | Expression, crystallization and preliminary crystallographic study of the C-terminal half of nsp2 from SARS coronavirus |
title_fullStr | Expression, crystallization and preliminary crystallographic study of the C-terminal half of nsp2 from SARS coronavirus |
title_full_unstemmed | Expression, crystallization and preliminary crystallographic study of the C-terminal half of nsp2 from SARS coronavirus |
title_short | Expression, crystallization and preliminary crystallographic study of the C-terminal half of nsp2 from SARS coronavirus |
title_sort | expression, crystallization and preliminary crystallographic study of the c-terminal half of nsp2 from sars coronavirus |
topic | Crystallization Communications |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3144797/ https://www.ncbi.nlm.nih.gov/pubmed/21795795 http://dx.doi.org/10.1107/S1744309111017829 |
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