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Expression, crystallization and preliminary crystallographic study of the C-terminal half of nsp2 from SARS coronavirus

SARS coronavirus (SARS-CoV) is the aetiological agent of the highly infectious severe acute respiratory syndrome (SARS). To gain a better understanding of SARS-CoV replication and transcription proteins, a preliminary X-ray crystallo­graphic study of the C-terminal domain of SARS-CoV nonstructural p...

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Autores principales: Li, Yuanyuan, Ren, Zhilin, Bao, Zehua, Ming, Zhenhua, Li, Xuemei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: International Union of Crystallography 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3144797/
https://www.ncbi.nlm.nih.gov/pubmed/21795795
http://dx.doi.org/10.1107/S1744309111017829
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author Li, Yuanyuan
Ren, Zhilin
Bao, Zehua
Ming, Zhenhua
Li, Xuemei
author_facet Li, Yuanyuan
Ren, Zhilin
Bao, Zehua
Ming, Zhenhua
Li, Xuemei
author_sort Li, Yuanyuan
collection PubMed
description SARS coronavirus (SARS-CoV) is the aetiological agent of the highly infectious severe acute respiratory syndrome (SARS). To gain a better understanding of SARS-CoV replication and transcription proteins, a preliminary X-ray crystallo­graphic study of the C-terminal domain of SARS-CoV nonstructural protein 2 (nsp2) is reported here. The C-­terminal domain of SARS-CoV nsp2 was cloned, overexpressed, purified and crystallized using polyethylene glycol 5000 monomethyl ether as the precipitant; the crystals diffracted to 2.5 Å resolution. The crystals belonged to space group P6(5), with unit-cell parameters a = b = 112.8, c = 91.1 Å, α = β = 90, γ = 120°. One molecule is assumed to be present per asymmetric unit, which gives a Matthews coefficient of 2.89 Å(3) Da(−1) and a solvent content of 56.2%.
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spelling pubmed-31447972013-07-01 Expression, crystallization and preliminary crystallographic study of the C-terminal half of nsp2 from SARS coronavirus Li, Yuanyuan Ren, Zhilin Bao, Zehua Ming, Zhenhua Li, Xuemei Acta Crystallogr Sect F Struct Biol Cryst Commun Crystallization Communications SARS coronavirus (SARS-CoV) is the aetiological agent of the highly infectious severe acute respiratory syndrome (SARS). To gain a better understanding of SARS-CoV replication and transcription proteins, a preliminary X-ray crystallo­graphic study of the C-terminal domain of SARS-CoV nonstructural protein 2 (nsp2) is reported here. The C-­terminal domain of SARS-CoV nsp2 was cloned, overexpressed, purified and crystallized using polyethylene glycol 5000 monomethyl ether as the precipitant; the crystals diffracted to 2.5 Å resolution. The crystals belonged to space group P6(5), with unit-cell parameters a = b = 112.8, c = 91.1 Å, α = β = 90, γ = 120°. One molecule is assumed to be present per asymmetric unit, which gives a Matthews coefficient of 2.89 Å(3) Da(−1) and a solvent content of 56.2%. International Union of Crystallography 2011-06-30 /pmc/articles/PMC3144797/ /pubmed/21795795 http://dx.doi.org/10.1107/S1744309111017829 Text en © International Union of Crystallography 2011
spellingShingle Crystallization Communications
Li, Yuanyuan
Ren, Zhilin
Bao, Zehua
Ming, Zhenhua
Li, Xuemei
Expression, crystallization and preliminary crystallographic study of the C-terminal half of nsp2 from SARS coronavirus
title Expression, crystallization and preliminary crystallographic study of the C-terminal half of nsp2 from SARS coronavirus
title_full Expression, crystallization and preliminary crystallographic study of the C-terminal half of nsp2 from SARS coronavirus
title_fullStr Expression, crystallization and preliminary crystallographic study of the C-terminal half of nsp2 from SARS coronavirus
title_full_unstemmed Expression, crystallization and preliminary crystallographic study of the C-terminal half of nsp2 from SARS coronavirus
title_short Expression, crystallization and preliminary crystallographic study of the C-terminal half of nsp2 from SARS coronavirus
title_sort expression, crystallization and preliminary crystallographic study of the c-terminal half of nsp2 from sars coronavirus
topic Crystallization Communications
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3144797/
https://www.ncbi.nlm.nih.gov/pubmed/21795795
http://dx.doi.org/10.1107/S1744309111017829
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