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An Experimental Model of Meningoencephalomyelitis by Rocio Flavivirus in Balb/C Mice: Inflammatory Response, Cytokine Production, and Histopathology

Rocio virus (ROCV) is a flavivirus, probably transmitted by Culex mosquitoes and maintained in nature as a zoonosis of wild birds. Rocio virus caused a human epidemic of severe encephalitis that lasted from 1973 to 1980 in the Ribeira valley, in the southeastern coast of Brazil. After this outbreak,...

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Autores principales: de Barros, Veridiana Ester Dias, Saggioro, Fabiano P., Neder, Luciano, de Oliveira França, Rafael Freitas, Mariguela, Viviane, Chávez, Juliana Helena, Penharvel, Sandra, Forjaz, Jorge, da Fonseca, Benedito Antônio Lopes, Figueiredo, Luiz Tadeu Moraes
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The American Society of Tropical Medicine and Hygiene 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3144838/
https://www.ncbi.nlm.nih.gov/pubmed/21813860
http://dx.doi.org/10.4269/ajtmh.2011.10-0246
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author de Barros, Veridiana Ester Dias
Saggioro, Fabiano P.
Neder, Luciano
de Oliveira França, Rafael Freitas
Mariguela, Viviane
Chávez, Juliana Helena
Penharvel, Sandra
Forjaz, Jorge
da Fonseca, Benedito Antônio Lopes
Figueiredo, Luiz Tadeu Moraes
author_facet de Barros, Veridiana Ester Dias
Saggioro, Fabiano P.
Neder, Luciano
de Oliveira França, Rafael Freitas
Mariguela, Viviane
Chávez, Juliana Helena
Penharvel, Sandra
Forjaz, Jorge
da Fonseca, Benedito Antônio Lopes
Figueiredo, Luiz Tadeu Moraes
author_sort de Barros, Veridiana Ester Dias
collection PubMed
description Rocio virus (ROCV) is a flavivirus, probably transmitted by Culex mosquitoes and maintained in nature as a zoonosis of wild birds. Rocio virus caused a human epidemic of severe encephalitis that lasted from 1973 to 1980 in the Ribeira valley, in the southeastern coast of Brazil. After this outbreak, serologic evidence of ROCV circulation has been reported and public health authorities are concerned about a return of ROCV outbreaks in Brazil. We show here a study on the pathogenesis and the physiopathology of ROCV disease in the central nervous system of a Balb/C young adult mice experimental model. The animals were intraperitoneally infected by ROCV and followed from 0 to 9 days after infection, when all of them died. Nervous tissue samples were collected from infected animals for immunohistochemistry and molecular biology analysis. We observed the virus in the central nervous system, the inflammatory changes induced by Th1 and Th2 cytokines, and the final irreversible damage of nervous tissues by neuronal degeneration and apoptosis. These findings can help to better understand the pathogenesis and physiopathology of the human meningoencephalomyelitis by ROCV and other flaviviruses.
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spelling pubmed-31448382011-08-09 An Experimental Model of Meningoencephalomyelitis by Rocio Flavivirus in Balb/C Mice: Inflammatory Response, Cytokine Production, and Histopathology de Barros, Veridiana Ester Dias Saggioro, Fabiano P. Neder, Luciano de Oliveira França, Rafael Freitas Mariguela, Viviane Chávez, Juliana Helena Penharvel, Sandra Forjaz, Jorge da Fonseca, Benedito Antônio Lopes Figueiredo, Luiz Tadeu Moraes Am J Trop Med Hyg Articles Rocio virus (ROCV) is a flavivirus, probably transmitted by Culex mosquitoes and maintained in nature as a zoonosis of wild birds. Rocio virus caused a human epidemic of severe encephalitis that lasted from 1973 to 1980 in the Ribeira valley, in the southeastern coast of Brazil. After this outbreak, serologic evidence of ROCV circulation has been reported and public health authorities are concerned about a return of ROCV outbreaks in Brazil. We show here a study on the pathogenesis and the physiopathology of ROCV disease in the central nervous system of a Balb/C young adult mice experimental model. The animals were intraperitoneally infected by ROCV and followed from 0 to 9 days after infection, when all of them died. Nervous tissue samples were collected from infected animals for immunohistochemistry and molecular biology analysis. We observed the virus in the central nervous system, the inflammatory changes induced by Th1 and Th2 cytokines, and the final irreversible damage of nervous tissues by neuronal degeneration and apoptosis. These findings can help to better understand the pathogenesis and physiopathology of the human meningoencephalomyelitis by ROCV and other flaviviruses. The American Society of Tropical Medicine and Hygiene 2011-08-01 /pmc/articles/PMC3144838/ /pubmed/21813860 http://dx.doi.org/10.4269/ajtmh.2011.10-0246 Text en ©The American Society of Tropical Medicine and Hygiene http://creativecommons.org/licenses/by/2.5/ This is an Open Access article distributed under the terms of the American Society of Tropical Medicine and Hygiene's Re-use License which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Articles
de Barros, Veridiana Ester Dias
Saggioro, Fabiano P.
Neder, Luciano
de Oliveira França, Rafael Freitas
Mariguela, Viviane
Chávez, Juliana Helena
Penharvel, Sandra
Forjaz, Jorge
da Fonseca, Benedito Antônio Lopes
Figueiredo, Luiz Tadeu Moraes
An Experimental Model of Meningoencephalomyelitis by Rocio Flavivirus in Balb/C Mice: Inflammatory Response, Cytokine Production, and Histopathology
title An Experimental Model of Meningoencephalomyelitis by Rocio Flavivirus in Balb/C Mice: Inflammatory Response, Cytokine Production, and Histopathology
title_full An Experimental Model of Meningoencephalomyelitis by Rocio Flavivirus in Balb/C Mice: Inflammatory Response, Cytokine Production, and Histopathology
title_fullStr An Experimental Model of Meningoencephalomyelitis by Rocio Flavivirus in Balb/C Mice: Inflammatory Response, Cytokine Production, and Histopathology
title_full_unstemmed An Experimental Model of Meningoencephalomyelitis by Rocio Flavivirus in Balb/C Mice: Inflammatory Response, Cytokine Production, and Histopathology
title_short An Experimental Model of Meningoencephalomyelitis by Rocio Flavivirus in Balb/C Mice: Inflammatory Response, Cytokine Production, and Histopathology
title_sort experimental model of meningoencephalomyelitis by rocio flavivirus in balb/c mice: inflammatory response, cytokine production, and histopathology
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3144838/
https://www.ncbi.nlm.nih.gov/pubmed/21813860
http://dx.doi.org/10.4269/ajtmh.2011.10-0246
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