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Three-Dimensional Imaging of the Mouse Neurovasculature with Magnetic Resonance Microscopy

Knowledge of the three-dimensional (3D) architecture of blood vessels in the brain is crucial because the progression of various neuropathologies ranging from Alzheimer's disease to brain tumors involves anomalous blood vessels. The challenges in obtaining such data from patients, in conjunctio...

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Detalles Bibliográficos
Autores principales: Pathak, Arvind P., Kim, Eugene, Zhang, Jiangyang, Jones, Melina V.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3144917/
https://www.ncbi.nlm.nih.gov/pubmed/21818357
http://dx.doi.org/10.1371/journal.pone.0022643
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author Pathak, Arvind P.
Kim, Eugene
Zhang, Jiangyang
Jones, Melina V.
author_facet Pathak, Arvind P.
Kim, Eugene
Zhang, Jiangyang
Jones, Melina V.
author_sort Pathak, Arvind P.
collection PubMed
description Knowledge of the three-dimensional (3D) architecture of blood vessels in the brain is crucial because the progression of various neuropathologies ranging from Alzheimer's disease to brain tumors involves anomalous blood vessels. The challenges in obtaining such data from patients, in conjunction with development of mouse models of neuropathology, have made the murine brain indispensable for investigating disease induced neurovascular changes. Here we describe a novel method for “whole brain” 3D mapping of murine neurovasculature using magnetic resonance microscopy (μMRI). This approach preserves the vascular and white matter tract architecture, and can be combined with complementary MRI contrast mechanisms such as diffusion tensor imaging (DTI) to examine the interplay between the vasculature and white matter reorganization that often characterizes neuropathologies. Following validation with micro computed tomography (μCT) and optical microscopy, we demonstrate the utility of this method by: (i) combined 3D imaging of angiogenesis and white matter reorganization in both, invasive and non-invasive brain tumor models; (ii) characterizing the morphological heterogeneity of the vascular phenotype in the murine brain; and (iii) conducting “multi-scale” imaging of brain tumor angiogenesis, wherein we directly compared in vivo MRI blood volume measurements with ex vivo vasculature data.
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spelling pubmed-31449172011-08-04 Three-Dimensional Imaging of the Mouse Neurovasculature with Magnetic Resonance Microscopy Pathak, Arvind P. Kim, Eugene Zhang, Jiangyang Jones, Melina V. PLoS One Research Article Knowledge of the three-dimensional (3D) architecture of blood vessels in the brain is crucial because the progression of various neuropathologies ranging from Alzheimer's disease to brain tumors involves anomalous blood vessels. The challenges in obtaining such data from patients, in conjunction with development of mouse models of neuropathology, have made the murine brain indispensable for investigating disease induced neurovascular changes. Here we describe a novel method for “whole brain” 3D mapping of murine neurovasculature using magnetic resonance microscopy (μMRI). This approach preserves the vascular and white matter tract architecture, and can be combined with complementary MRI contrast mechanisms such as diffusion tensor imaging (DTI) to examine the interplay between the vasculature and white matter reorganization that often characterizes neuropathologies. Following validation with micro computed tomography (μCT) and optical microscopy, we demonstrate the utility of this method by: (i) combined 3D imaging of angiogenesis and white matter reorganization in both, invasive and non-invasive brain tumor models; (ii) characterizing the morphological heterogeneity of the vascular phenotype in the murine brain; and (iii) conducting “multi-scale” imaging of brain tumor angiogenesis, wherein we directly compared in vivo MRI blood volume measurements with ex vivo vasculature data. Public Library of Science 2011-07-27 /pmc/articles/PMC3144917/ /pubmed/21818357 http://dx.doi.org/10.1371/journal.pone.0022643 Text en Pathak et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Pathak, Arvind P.
Kim, Eugene
Zhang, Jiangyang
Jones, Melina V.
Three-Dimensional Imaging of the Mouse Neurovasculature with Magnetic Resonance Microscopy
title Three-Dimensional Imaging of the Mouse Neurovasculature with Magnetic Resonance Microscopy
title_full Three-Dimensional Imaging of the Mouse Neurovasculature with Magnetic Resonance Microscopy
title_fullStr Three-Dimensional Imaging of the Mouse Neurovasculature with Magnetic Resonance Microscopy
title_full_unstemmed Three-Dimensional Imaging of the Mouse Neurovasculature with Magnetic Resonance Microscopy
title_short Three-Dimensional Imaging of the Mouse Neurovasculature with Magnetic Resonance Microscopy
title_sort three-dimensional imaging of the mouse neurovasculature with magnetic resonance microscopy
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3144917/
https://www.ncbi.nlm.nih.gov/pubmed/21818357
http://dx.doi.org/10.1371/journal.pone.0022643
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