Native-State Stability Determines the Extent of Degradation Relative to Secretion of Protein Variants from Pichia pastoris

We have investigated the relationship between the stability and secreted yield of a series of mutational variants of human lysozyme (HuL) in Pichia pastoris. We show that genes directly involved in the unfolded protein response (UPR), ER-associated degradation (ERAD) and ER-phagy are transcriptional...

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Detalles Bibliográficos
Autores principales: Whyteside, Graham, Alcocer, Marcos J. C., Kumita, Janet R., Dobson, Christopher M., Lazarou, Maria, Pleass, Richard J., Archer, David B.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2011
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3144928/
https://www.ncbi.nlm.nih.gov/pubmed/21818368
http://dx.doi.org/10.1371/journal.pone.0022692

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