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Overlapping kinetochore targets of CK2 and Aurora B kinases in mitotic regulation

Protein kinase CK2 is one of the most conserved kinases in eukaryotic cells and plays essential roles in diverse processes. While we know that CK2 plays a role(s) in cell division, our understanding of how CK2 regulates cell cycle progression is limited. In this study, we revealed a regulatory role...

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Autores principales: Peng, Yutian, Wong, Catherine C. L., Nakajima, Yuko, Tyers, Randall G., Sarkeshik, Ali S., Yates, John, Drubin, David G., Barnes, Georjana
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The American Society for Cell Biology 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3145544/
https://www.ncbi.nlm.nih.gov/pubmed/21633108
http://dx.doi.org/10.1091/mbc.E10-11-0915
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author Peng, Yutian
Wong, Catherine C. L.
Nakajima, Yuko
Tyers, Randall G.
Sarkeshik, Ali S.
Yates, John
Drubin, David G.
Barnes, Georjana
author_facet Peng, Yutian
Wong, Catherine C. L.
Nakajima, Yuko
Tyers, Randall G.
Sarkeshik, Ali S.
Yates, John
Drubin, David G.
Barnes, Georjana
author_sort Peng, Yutian
collection PubMed
description Protein kinase CK2 is one of the most conserved kinases in eukaryotic cells and plays essential roles in diverse processes. While we know that CK2 plays a role(s) in cell division, our understanding of how CK2 regulates cell cycle progression is limited. In this study, we revealed a regulatory role for CK2 in kinetochore function. The kinetochore is a multi-protein complex that assembles on the centromere of a chromosome and functions to attach chromosomes to spindle microtubules. To faithfully segregate chromosomes and maintain genomic integrity, the kinetochore is tightly regulated by multiple mechanisms, including phosphorylation by Aurora B kinase. We found that a loss of CK2 kinase activity inhibits anaphase spindle elongation and results in chromosome missegregation. Moreover, a lack of CK2 activates the spindle assembly checkpoint. We demonstrate that CK2 associates with Mif2, the Saccharomyces cerevisiae homologue of human CENP-C, which serves as an important link between the inner and outer kinetochore. Furthermore, we show Mif2 and the inner kinetochore protein Ndc10 are phosphorylated by CK2, and this phosphorylation plays antagonistic and synergistic roles with Aurora B phosphorylation of these targets, respectively.
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spelling pubmed-31455442011-10-16 Overlapping kinetochore targets of CK2 and Aurora B kinases in mitotic regulation Peng, Yutian Wong, Catherine C. L. Nakajima, Yuko Tyers, Randall G. Sarkeshik, Ali S. Yates, John Drubin, David G. Barnes, Georjana Mol Biol Cell Articles Protein kinase CK2 is one of the most conserved kinases in eukaryotic cells and plays essential roles in diverse processes. While we know that CK2 plays a role(s) in cell division, our understanding of how CK2 regulates cell cycle progression is limited. In this study, we revealed a regulatory role for CK2 in kinetochore function. The kinetochore is a multi-protein complex that assembles on the centromere of a chromosome and functions to attach chromosomes to spindle microtubules. To faithfully segregate chromosomes and maintain genomic integrity, the kinetochore is tightly regulated by multiple mechanisms, including phosphorylation by Aurora B kinase. We found that a loss of CK2 kinase activity inhibits anaphase spindle elongation and results in chromosome missegregation. Moreover, a lack of CK2 activates the spindle assembly checkpoint. We demonstrate that CK2 associates with Mif2, the Saccharomyces cerevisiae homologue of human CENP-C, which serves as an important link between the inner and outer kinetochore. Furthermore, we show Mif2 and the inner kinetochore protein Ndc10 are phosphorylated by CK2, and this phosphorylation plays antagonistic and synergistic roles with Aurora B phosphorylation of these targets, respectively. The American Society for Cell Biology 2011-08-01 /pmc/articles/PMC3145544/ /pubmed/21633108 http://dx.doi.org/10.1091/mbc.E10-11-0915 Text en © 2011 Peng et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0). “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society of Cell Biology.
spellingShingle Articles
Peng, Yutian
Wong, Catherine C. L.
Nakajima, Yuko
Tyers, Randall G.
Sarkeshik, Ali S.
Yates, John
Drubin, David G.
Barnes, Georjana
Overlapping kinetochore targets of CK2 and Aurora B kinases in mitotic regulation
title Overlapping kinetochore targets of CK2 and Aurora B kinases in mitotic regulation
title_full Overlapping kinetochore targets of CK2 and Aurora B kinases in mitotic regulation
title_fullStr Overlapping kinetochore targets of CK2 and Aurora B kinases in mitotic regulation
title_full_unstemmed Overlapping kinetochore targets of CK2 and Aurora B kinases in mitotic regulation
title_short Overlapping kinetochore targets of CK2 and Aurora B kinases in mitotic regulation
title_sort overlapping kinetochore targets of ck2 and aurora b kinases in mitotic regulation
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3145544/
https://www.ncbi.nlm.nih.gov/pubmed/21633108
http://dx.doi.org/10.1091/mbc.E10-11-0915
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