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Morphological and Molecular Analysis of the Nematostella vectensis Cnidom
The starlet sea anemone Nematostella vectensis is an emerging model organism for developmental and evolutionary biology. Due to the availability of genome data and its amenability to genetic manipulation Nematostella serves as a source for comparative molecular and phylogenetic studies. Despite this...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3145756/ https://www.ncbi.nlm.nih.gov/pubmed/21829492 http://dx.doi.org/10.1371/journal.pone.0022725 |
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author | Zenkert, Claudia Takahashi, Toshio Diesner, Mark-Oliver Özbek, Suat |
author_facet | Zenkert, Claudia Takahashi, Toshio Diesner, Mark-Oliver Özbek, Suat |
author_sort | Zenkert, Claudia |
collection | PubMed |
description | The starlet sea anemone Nematostella vectensis is an emerging model organism for developmental and evolutionary biology. Due to the availability of genome data and its amenability to genetic manipulation Nematostella serves as a source for comparative molecular and phylogenetic studies. Despite this fact, the characterization of the nematocyst inventory and of nematocyst-specific genes is still fragmentary and sometimes misleading in this cnidarian species. Here, we present a thorough qualitative and quantitative analysis of nematocysts in Nematostella vectensis. In addition, we have cloned major nematocyst components, Nematostella minicollagens 1, 3 and 4, and show their expression patterns by in situ hybridization and immunocytochemistry using specific antibodies. Our data provides tools and insights for further studies on nematocyst morphogenesis in Nematostella and comparative evolution in cnidarians. |
format | Online Article Text |
id | pubmed-3145756 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-31457562011-08-09 Morphological and Molecular Analysis of the Nematostella vectensis Cnidom Zenkert, Claudia Takahashi, Toshio Diesner, Mark-Oliver Özbek, Suat PLoS One Research Article The starlet sea anemone Nematostella vectensis is an emerging model organism for developmental and evolutionary biology. Due to the availability of genome data and its amenability to genetic manipulation Nematostella serves as a source for comparative molecular and phylogenetic studies. Despite this fact, the characterization of the nematocyst inventory and of nematocyst-specific genes is still fragmentary and sometimes misleading in this cnidarian species. Here, we present a thorough qualitative and quantitative analysis of nematocysts in Nematostella vectensis. In addition, we have cloned major nematocyst components, Nematostella minicollagens 1, 3 and 4, and show their expression patterns by in situ hybridization and immunocytochemistry using specific antibodies. Our data provides tools and insights for further studies on nematocyst morphogenesis in Nematostella and comparative evolution in cnidarians. Public Library of Science 2011-07-28 /pmc/articles/PMC3145756/ /pubmed/21829492 http://dx.doi.org/10.1371/journal.pone.0022725 Text en Zenkert et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Zenkert, Claudia Takahashi, Toshio Diesner, Mark-Oliver Özbek, Suat Morphological and Molecular Analysis of the Nematostella vectensis Cnidom |
title | Morphological and Molecular Analysis of the Nematostella vectensis Cnidom |
title_full | Morphological and Molecular Analysis of the Nematostella vectensis Cnidom |
title_fullStr | Morphological and Molecular Analysis of the Nematostella vectensis Cnidom |
title_full_unstemmed | Morphological and Molecular Analysis of the Nematostella vectensis Cnidom |
title_short | Morphological and Molecular Analysis of the Nematostella vectensis Cnidom |
title_sort | morphological and molecular analysis of the nematostella vectensis cnidom |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3145756/ https://www.ncbi.nlm.nih.gov/pubmed/21829492 http://dx.doi.org/10.1371/journal.pone.0022725 |
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