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The Regenerative Capacity of the Zebrafish Caudal Fin Is Not Affected by Repeated Amputations
BACKGROUND: The zebrafish has the capacity to regenerate many tissues and organs. The caudal fin is one of the most convenient tissues to approach experimentally due to its accessibility, simple structure and fast regeneration. In this work we investigate how the regenerative capacity is affected by...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3145768/ https://www.ncbi.nlm.nih.gov/pubmed/21829525 http://dx.doi.org/10.1371/journal.pone.0022820 |
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author | Azevedo, Ana Sofia Grotek, Bartholomäus Jacinto, António Weidinger, Gilbert Saúde, Leonor |
author_facet | Azevedo, Ana Sofia Grotek, Bartholomäus Jacinto, António Weidinger, Gilbert Saúde, Leonor |
author_sort | Azevedo, Ana Sofia |
collection | PubMed |
description | BACKGROUND: The zebrafish has the capacity to regenerate many tissues and organs. The caudal fin is one of the most convenient tissues to approach experimentally due to its accessibility, simple structure and fast regeneration. In this work we investigate how the regenerative capacity is affected by recurrent fin amputations and by experimental manipulations that block regeneration. METHODOLOGY/PRINCIPAL FINDINGS: We show that consecutive repeated amputations of zebrafish caudal fin do not reduce its regeneration capacity and do not compromise any of the successive regeneration steps: wound healing, blastema formation and regenerative outgrowth. Interfering with Wnt/ß-catenin signalling using heat-shock-mediated overexpression of Dickkopf1 completely blocks fin regeneration. Notably, if these fins were re-amputated at the non-inhibitory temperature, the regenerated caudal fin reached the original length, even after several rounds of consecutive Wnt/ß-catenin signalling inhibition and re-amputation. CONCLUSIONS/SIGNIFICANCE: We show that the caudal fin has an almost unlimited capacity to regenerate. Even after inhibition of regeneration caused by the loss of Wnt/ß-catenin signalling, a new amputation resets the regeneration capacity within the caudal fin, suggesting that blastema formation does not depend on a pool of stem/progenitor cells that require Wnt/ß-catenin signalling for their survival. |
format | Online Article Text |
id | pubmed-3145768 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-31457682011-08-09 The Regenerative Capacity of the Zebrafish Caudal Fin Is Not Affected by Repeated Amputations Azevedo, Ana Sofia Grotek, Bartholomäus Jacinto, António Weidinger, Gilbert Saúde, Leonor PLoS One Research Article BACKGROUND: The zebrafish has the capacity to regenerate many tissues and organs. The caudal fin is one of the most convenient tissues to approach experimentally due to its accessibility, simple structure and fast regeneration. In this work we investigate how the regenerative capacity is affected by recurrent fin amputations and by experimental manipulations that block regeneration. METHODOLOGY/PRINCIPAL FINDINGS: We show that consecutive repeated amputations of zebrafish caudal fin do not reduce its regeneration capacity and do not compromise any of the successive regeneration steps: wound healing, blastema formation and regenerative outgrowth. Interfering with Wnt/ß-catenin signalling using heat-shock-mediated overexpression of Dickkopf1 completely blocks fin regeneration. Notably, if these fins were re-amputated at the non-inhibitory temperature, the regenerated caudal fin reached the original length, even after several rounds of consecutive Wnt/ß-catenin signalling inhibition and re-amputation. CONCLUSIONS/SIGNIFICANCE: We show that the caudal fin has an almost unlimited capacity to regenerate. Even after inhibition of regeneration caused by the loss of Wnt/ß-catenin signalling, a new amputation resets the regeneration capacity within the caudal fin, suggesting that blastema formation does not depend on a pool of stem/progenitor cells that require Wnt/ß-catenin signalling for their survival. Public Library of Science 2011-07-28 /pmc/articles/PMC3145768/ /pubmed/21829525 http://dx.doi.org/10.1371/journal.pone.0022820 Text en Azevedo et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Azevedo, Ana Sofia Grotek, Bartholomäus Jacinto, António Weidinger, Gilbert Saúde, Leonor The Regenerative Capacity of the Zebrafish Caudal Fin Is Not Affected by Repeated Amputations |
title | The Regenerative Capacity of the Zebrafish Caudal Fin Is Not Affected by Repeated Amputations |
title_full | The Regenerative Capacity of the Zebrafish Caudal Fin Is Not Affected by Repeated Amputations |
title_fullStr | The Regenerative Capacity of the Zebrafish Caudal Fin Is Not Affected by Repeated Amputations |
title_full_unstemmed | The Regenerative Capacity of the Zebrafish Caudal Fin Is Not Affected by Repeated Amputations |
title_short | The Regenerative Capacity of the Zebrafish Caudal Fin Is Not Affected by Repeated Amputations |
title_sort | regenerative capacity of the zebrafish caudal fin is not affected by repeated amputations |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3145768/ https://www.ncbi.nlm.nih.gov/pubmed/21829525 http://dx.doi.org/10.1371/journal.pone.0022820 |
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