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Quantification of cAMP and cGMP analogs in intact cells: pitfalls in enzyme immunoassays for cyclic nucleotides

Immunoassays are routinely used as research tools to measure intracellular cAMP and cGMP concentrations. Ideally, this application requires antibodies with high sensitivity and specificity. The present work evaluates the cross-reactivity of commercially available cyclic nucleotide analogs with two n...

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Detalles Bibliográficos
Autores principales: Werner, Katharina, Schwede, Frank, Genieser, Hans-Gottfried, Geiger, Jörg, Butt, Elke
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer-Verlag 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3145891/
https://www.ncbi.nlm.nih.gov/pubmed/21713381
http://dx.doi.org/10.1007/s00210-011-0662-6