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Comparative Analysis of Korean Human Gut Microbiota by Barcoded Pyrosequencing

Human gut microbiota plays important roles in harvesting energy from the diet, stimulating the proliferation of the intestinal epithelium, developing the immune system, and regulating fat storage in the host. Characterization of gut microbiota, however, has been limited to western people and is not...

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Autores principales: Nam, Young-Do, Jung, Mi-Ja, Roh, Seong Woon, Kim, Min-Soo, Bae, Jin-Woo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3146482/
https://www.ncbi.nlm.nih.gov/pubmed/21829445
http://dx.doi.org/10.1371/journal.pone.0022109
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author Nam, Young-Do
Jung, Mi-Ja
Roh, Seong Woon
Kim, Min-Soo
Bae, Jin-Woo
author_facet Nam, Young-Do
Jung, Mi-Ja
Roh, Seong Woon
Kim, Min-Soo
Bae, Jin-Woo
author_sort Nam, Young-Do
collection PubMed
description Human gut microbiota plays important roles in harvesting energy from the diet, stimulating the proliferation of the intestinal epithelium, developing the immune system, and regulating fat storage in the host. Characterization of gut microbiota, however, has been limited to western people and is not sufficiently extensive to fully describe microbial communities. In this study, we investigated the overall composition of the gut microbiota and its host specificity and temporal stability in 20 Koreans using 454-pyrosequencing with barcoded primers targeting the V1 to V3 region of the bacterial 16S rRNA gene. A total of 303,402 high quality reads covered each sample and 8,427 reads were analyzed on average. The results were compared with those of individuals from the USA, China and Japan. In general, microbial communities were dominated by five previously identified phyla: Actinobacteria, Firmicutes, Bacteroidetes, Fusobacteria, and Proteobacteria. UPGMA cluster analysis showed that the species composition of gut microbiota was host-specific and stable over the duration of the test period, but the relative abundance of each member fluctuated. 43 core Korean gut microbiota were identified by comparison of sequences from each individual, of which 15 species level phylotypes were related to previously-reported butyrate-producing bacteria. UniFrac analysis revealed that human gut microbiota differed between countries: Korea, USA, Japan and China, but tended to vary less between individual Koreans, suggesting that gut microbial composition is related to internal and external characteristics of each country member such as host genetics and diet styles.
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spelling pubmed-31464822011-08-09 Comparative Analysis of Korean Human Gut Microbiota by Barcoded Pyrosequencing Nam, Young-Do Jung, Mi-Ja Roh, Seong Woon Kim, Min-Soo Bae, Jin-Woo PLoS One Research Article Human gut microbiota plays important roles in harvesting energy from the diet, stimulating the proliferation of the intestinal epithelium, developing the immune system, and regulating fat storage in the host. Characterization of gut microbiota, however, has been limited to western people and is not sufficiently extensive to fully describe microbial communities. In this study, we investigated the overall composition of the gut microbiota and its host specificity and temporal stability in 20 Koreans using 454-pyrosequencing with barcoded primers targeting the V1 to V3 region of the bacterial 16S rRNA gene. A total of 303,402 high quality reads covered each sample and 8,427 reads were analyzed on average. The results were compared with those of individuals from the USA, China and Japan. In general, microbial communities were dominated by five previously identified phyla: Actinobacteria, Firmicutes, Bacteroidetes, Fusobacteria, and Proteobacteria. UPGMA cluster analysis showed that the species composition of gut microbiota was host-specific and stable over the duration of the test period, but the relative abundance of each member fluctuated. 43 core Korean gut microbiota were identified by comparison of sequences from each individual, of which 15 species level phylotypes were related to previously-reported butyrate-producing bacteria. UniFrac analysis revealed that human gut microbiota differed between countries: Korea, USA, Japan and China, but tended to vary less between individual Koreans, suggesting that gut microbial composition is related to internal and external characteristics of each country member such as host genetics and diet styles. Public Library of Science 2011-07-29 /pmc/articles/PMC3146482/ /pubmed/21829445 http://dx.doi.org/10.1371/journal.pone.0022109 Text en Nam et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Nam, Young-Do
Jung, Mi-Ja
Roh, Seong Woon
Kim, Min-Soo
Bae, Jin-Woo
Comparative Analysis of Korean Human Gut Microbiota by Barcoded Pyrosequencing
title Comparative Analysis of Korean Human Gut Microbiota by Barcoded Pyrosequencing
title_full Comparative Analysis of Korean Human Gut Microbiota by Barcoded Pyrosequencing
title_fullStr Comparative Analysis of Korean Human Gut Microbiota by Barcoded Pyrosequencing
title_full_unstemmed Comparative Analysis of Korean Human Gut Microbiota by Barcoded Pyrosequencing
title_short Comparative Analysis of Korean Human Gut Microbiota by Barcoded Pyrosequencing
title_sort comparative analysis of korean human gut microbiota by barcoded pyrosequencing
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3146482/
https://www.ncbi.nlm.nih.gov/pubmed/21829445
http://dx.doi.org/10.1371/journal.pone.0022109
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