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Reproducible gene targeting in recalcitrant Escherichia coli isolates
BACKGROUND: A number of allele replacement methods can be used to mutate bacterial genes. For instance, the Red recombinase system of phage Lambda has been used very efficiently to inactivate chromosomal genes in E. coli K-12, through recombination between regions of homology. However, this method d...
| Autores principales: | , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
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BioMed Central
2011
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3146853/ https://www.ncbi.nlm.nih.gov/pubmed/21696605 http://dx.doi.org/10.1186/1756-0500-4-213 |