Modeling and measuring intracellular fluxes of secreted recombinant protein in Pichia pastoris with a novel (34)S labeling procedure

BACKGROUND: The budding yeast Pichia pastoris is widely used for protein production. To determine the best suitable strategy for strain improvement, especially for high secretion, quantitative data of intracellular fluxes of recombinant protein are very important. Especially the balance between intr...

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Detalles Bibliográficos
Autores principales: Pfeffer, Martin, Maurer, Michael, Köllensperger, Gunda, Hann, Stephan, Graf, Alexandra B, Mattanovich, Diethard
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2011
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3147017/
https://www.ncbi.nlm.nih.gov/pubmed/21703020
http://dx.doi.org/10.1186/1475-2859-10-47

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