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Nitric oxide is required for an optimal establishment of the Medicago truncatula–Sinorhizobium meliloti symbiosis
Nitric oxide (NO) is a gaseous molecule that participates in numerous plant signalling pathways. It is involved in plant responses to pathogens and development processes such as seed germination, flowering and stomatal closure. Using a permeable NO-specific fluorescent probe and a bacterial reporter...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Blackwell Publishing Ltd
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3147055/ https://www.ncbi.nlm.nih.gov/pubmed/21457261 http://dx.doi.org/10.1111/j.1469-8137.2011.03693.x |
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author | del Giudice, Jennifer Cam, Yvan Damiani, Isabelle Fung-Chat, Franck Meilhoc, Eliane Bruand, Claude Brouquisse, Renaud Puppo, Alain Boscari, Alexandre |
author_facet | del Giudice, Jennifer Cam, Yvan Damiani, Isabelle Fung-Chat, Franck Meilhoc, Eliane Bruand, Claude Brouquisse, Renaud Puppo, Alain Boscari, Alexandre |
author_sort | del Giudice, Jennifer |
collection | PubMed |
description | Nitric oxide (NO) is a gaseous molecule that participates in numerous plant signalling pathways. It is involved in plant responses to pathogens and development processes such as seed germination, flowering and stomatal closure. Using a permeable NO-specific fluorescent probe and a bacterial reporter strain expressing the lacZ gene under the control of a NO-responsive promoter, we detected NO production in the first steps, during infection threads growth, of the Medicago truncatula–Sinorhizobium meliloti symbiotic interaction. Nitric oxide was also detected, by confocal microscopy, in nodule primordia. Depletion of NO caused by cPTIO (2-(4-carboxyphenyl)-4,4,5,5-tetramethyl imidazoline-1-oxyl-3-oxide), an NO scavenger, resulted in a significant delay in nodule appearance. The overexpression of a bacterial hmp gene, encoding a flavohaemoglobin able to scavenge NO, under the control of a nodule-specific promoter (pENOD20) in transgenic roots, led to the same phenotype. The NO scavenging resulting from these approaches provoked the downregulation of plant genes involved in nodule development, such as MtCRE1 and MtCCS52A. Furthermore, an Hmp-overexpressing S. meliloti mutant strain was found to be less competitive than the wild type in the nodulation process. Taken together, these results indicate that NO is required for an optimal establishment of the M. truncatula–S. meliloti symbiotic interaction. |
format | Online Article Text |
id | pubmed-3147055 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | Blackwell Publishing Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-31470552011-08-03 Nitric oxide is required for an optimal establishment of the Medicago truncatula–Sinorhizobium meliloti symbiosis del Giudice, Jennifer Cam, Yvan Damiani, Isabelle Fung-Chat, Franck Meilhoc, Eliane Bruand, Claude Brouquisse, Renaud Puppo, Alain Boscari, Alexandre New Phytol Research Nitric oxide (NO) is a gaseous molecule that participates in numerous plant signalling pathways. It is involved in plant responses to pathogens and development processes such as seed germination, flowering and stomatal closure. Using a permeable NO-specific fluorescent probe and a bacterial reporter strain expressing the lacZ gene under the control of a NO-responsive promoter, we detected NO production in the first steps, during infection threads growth, of the Medicago truncatula–Sinorhizobium meliloti symbiotic interaction. Nitric oxide was also detected, by confocal microscopy, in nodule primordia. Depletion of NO caused by cPTIO (2-(4-carboxyphenyl)-4,4,5,5-tetramethyl imidazoline-1-oxyl-3-oxide), an NO scavenger, resulted in a significant delay in nodule appearance. The overexpression of a bacterial hmp gene, encoding a flavohaemoglobin able to scavenge NO, under the control of a nodule-specific promoter (pENOD20) in transgenic roots, led to the same phenotype. The NO scavenging resulting from these approaches provoked the downregulation of plant genes involved in nodule development, such as MtCRE1 and MtCCS52A. Furthermore, an Hmp-overexpressing S. meliloti mutant strain was found to be less competitive than the wild type in the nodulation process. Taken together, these results indicate that NO is required for an optimal establishment of the M. truncatula–S. meliloti symbiotic interaction. Blackwell Publishing Ltd 2011-07 /pmc/articles/PMC3147055/ /pubmed/21457261 http://dx.doi.org/10.1111/j.1469-8137.2011.03693.x Text en Copyright © 2011 New Phytologist Trust http://creativecommons.org/licenses/by/2.5/ Re-use of this article is permitted in accordance with the Creative Commons Deed, Attribution 2.5, which does not permit commercial exploitation. |
spellingShingle | Research del Giudice, Jennifer Cam, Yvan Damiani, Isabelle Fung-Chat, Franck Meilhoc, Eliane Bruand, Claude Brouquisse, Renaud Puppo, Alain Boscari, Alexandre Nitric oxide is required for an optimal establishment of the Medicago truncatula–Sinorhizobium meliloti symbiosis |
title | Nitric oxide is required for an optimal establishment of the Medicago truncatula–Sinorhizobium meliloti symbiosis |
title_full | Nitric oxide is required for an optimal establishment of the Medicago truncatula–Sinorhizobium meliloti symbiosis |
title_fullStr | Nitric oxide is required for an optimal establishment of the Medicago truncatula–Sinorhizobium meliloti symbiosis |
title_full_unstemmed | Nitric oxide is required for an optimal establishment of the Medicago truncatula–Sinorhizobium meliloti symbiosis |
title_short | Nitric oxide is required for an optimal establishment of the Medicago truncatula–Sinorhizobium meliloti symbiosis |
title_sort | nitric oxide is required for an optimal establishment of the medicago truncatula–sinorhizobium meliloti symbiosis |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3147055/ https://www.ncbi.nlm.nih.gov/pubmed/21457261 http://dx.doi.org/10.1111/j.1469-8137.2011.03693.x |
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