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Nitric oxide is required for an optimal establishment of the Medicago truncatula–Sinorhizobium meliloti symbiosis

Nitric oxide (NO) is a gaseous molecule that participates in numerous plant signalling pathways. It is involved in plant responses to pathogens and development processes such as seed germination, flowering and stomatal closure. Using a permeable NO-specific fluorescent probe and a bacterial reporter...

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Autores principales: del Giudice, Jennifer, Cam, Yvan, Damiani, Isabelle, Fung-Chat, Franck, Meilhoc, Eliane, Bruand, Claude, Brouquisse, Renaud, Puppo, Alain, Boscari, Alexandre
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Blackwell Publishing Ltd 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3147055/
https://www.ncbi.nlm.nih.gov/pubmed/21457261
http://dx.doi.org/10.1111/j.1469-8137.2011.03693.x
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author del Giudice, Jennifer
Cam, Yvan
Damiani, Isabelle
Fung-Chat, Franck
Meilhoc, Eliane
Bruand, Claude
Brouquisse, Renaud
Puppo, Alain
Boscari, Alexandre
author_facet del Giudice, Jennifer
Cam, Yvan
Damiani, Isabelle
Fung-Chat, Franck
Meilhoc, Eliane
Bruand, Claude
Brouquisse, Renaud
Puppo, Alain
Boscari, Alexandre
author_sort del Giudice, Jennifer
collection PubMed
description Nitric oxide (NO) is a gaseous molecule that participates in numerous plant signalling pathways. It is involved in plant responses to pathogens and development processes such as seed germination, flowering and stomatal closure. Using a permeable NO-specific fluorescent probe and a bacterial reporter strain expressing the lacZ gene under the control of a NO-responsive promoter, we detected NO production in the first steps, during infection threads growth, of the Medicago truncatula–Sinorhizobium meliloti symbiotic interaction. Nitric oxide was also detected, by confocal microscopy, in nodule primordia. Depletion of NO caused by cPTIO (2-(4-carboxyphenyl)-4,4,5,5-tetramethyl imidazoline-1-oxyl-3-oxide), an NO scavenger, resulted in a significant delay in nodule appearance. The overexpression of a bacterial hmp gene, encoding a flavohaemoglobin able to scavenge NO, under the control of a nodule-specific promoter (pENOD20) in transgenic roots, led to the same phenotype. The NO scavenging resulting from these approaches provoked the downregulation of plant genes involved in nodule development, such as MtCRE1 and MtCCS52A. Furthermore, an Hmp-overexpressing S. meliloti mutant strain was found to be less competitive than the wild type in the nodulation process. Taken together, these results indicate that NO is required for an optimal establishment of the M. truncatula–S. meliloti symbiotic interaction.
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spelling pubmed-31470552011-08-03 Nitric oxide is required for an optimal establishment of the Medicago truncatula–Sinorhizobium meliloti symbiosis del Giudice, Jennifer Cam, Yvan Damiani, Isabelle Fung-Chat, Franck Meilhoc, Eliane Bruand, Claude Brouquisse, Renaud Puppo, Alain Boscari, Alexandre New Phytol Research Nitric oxide (NO) is a gaseous molecule that participates in numerous plant signalling pathways. It is involved in plant responses to pathogens and development processes such as seed germination, flowering and stomatal closure. Using a permeable NO-specific fluorescent probe and a bacterial reporter strain expressing the lacZ gene under the control of a NO-responsive promoter, we detected NO production in the first steps, during infection threads growth, of the Medicago truncatula–Sinorhizobium meliloti symbiotic interaction. Nitric oxide was also detected, by confocal microscopy, in nodule primordia. Depletion of NO caused by cPTIO (2-(4-carboxyphenyl)-4,4,5,5-tetramethyl imidazoline-1-oxyl-3-oxide), an NO scavenger, resulted in a significant delay in nodule appearance. The overexpression of a bacterial hmp gene, encoding a flavohaemoglobin able to scavenge NO, under the control of a nodule-specific promoter (pENOD20) in transgenic roots, led to the same phenotype. The NO scavenging resulting from these approaches provoked the downregulation of plant genes involved in nodule development, such as MtCRE1 and MtCCS52A. Furthermore, an Hmp-overexpressing S. meliloti mutant strain was found to be less competitive than the wild type in the nodulation process. Taken together, these results indicate that NO is required for an optimal establishment of the M. truncatula–S. meliloti symbiotic interaction. Blackwell Publishing Ltd 2011-07 /pmc/articles/PMC3147055/ /pubmed/21457261 http://dx.doi.org/10.1111/j.1469-8137.2011.03693.x Text en Copyright © 2011 New Phytologist Trust http://creativecommons.org/licenses/by/2.5/ Re-use of this article is permitted in accordance with the Creative Commons Deed, Attribution 2.5, which does not permit commercial exploitation.
spellingShingle Research
del Giudice, Jennifer
Cam, Yvan
Damiani, Isabelle
Fung-Chat, Franck
Meilhoc, Eliane
Bruand, Claude
Brouquisse, Renaud
Puppo, Alain
Boscari, Alexandre
Nitric oxide is required for an optimal establishment of the Medicago truncatula–Sinorhizobium meliloti symbiosis
title Nitric oxide is required for an optimal establishment of the Medicago truncatula–Sinorhizobium meliloti symbiosis
title_full Nitric oxide is required for an optimal establishment of the Medicago truncatula–Sinorhizobium meliloti symbiosis
title_fullStr Nitric oxide is required for an optimal establishment of the Medicago truncatula–Sinorhizobium meliloti symbiosis
title_full_unstemmed Nitric oxide is required for an optimal establishment of the Medicago truncatula–Sinorhizobium meliloti symbiosis
title_short Nitric oxide is required for an optimal establishment of the Medicago truncatula–Sinorhizobium meliloti symbiosis
title_sort nitric oxide is required for an optimal establishment of the medicago truncatula–sinorhizobium meliloti symbiosis
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3147055/
https://www.ncbi.nlm.nih.gov/pubmed/21457261
http://dx.doi.org/10.1111/j.1469-8137.2011.03693.x
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