Assessment of Bioflocculant Production by Bacillus sp. Gilbert, a Marine Bacterium Isolated from the Bottom Sediment of Algoa Bay

The bioflocculant-producing potentials of a marine bacteria isolated from the bottom sediment of Algoa Bay was investigated using standard methods. The 16S rDNA sequence analysis revealed 98% similarity to that of Bacillus sp. HXG-C1 and the nucleotide sequence was deposited in GenBank as Bacillus s...

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Detalles Bibliográficos
Autores principales: Nontembiso, Piyo, Sekelwa, Cosa, Leonard, Mabinya V., Anthony, Okoh I.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Molecular Diversity Preservation International 2011
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3148500/
https://www.ncbi.nlm.nih.gov/pubmed/21822413
http://dx.doi.org/10.3390/md9071232
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author Nontembiso, Piyo
Sekelwa, Cosa
Leonard, Mabinya V.
Anthony, Okoh I.
author_facet Nontembiso, Piyo
Sekelwa, Cosa
Leonard, Mabinya V.
Anthony, Okoh I.
author_sort Nontembiso, Piyo
collection PubMed
description The bioflocculant-producing potentials of a marine bacteria isolated from the bottom sediment of Algoa Bay was investigated using standard methods. The 16S rDNA sequence analysis revealed 98% similarity to that of Bacillus sp. HXG-C1 and the nucleotide sequence was deposited in GenBank as Bacillus sp. Gilbert with accession number HQ537128. Bioflocculant was optimally produced when sucrose (72% flocculating activity) and ammonium chloride (91% flocculating activity) were used as sole sources of carbon and nitrogen, respectively; an initial pH 6.2 of the production medium; and Mg(2+) as cation. Chemical analysis of the purified bioflocculant revealed the compound to be a polysaccharide.
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spelling pubmed-31485002011-08-05 Assessment of Bioflocculant Production by Bacillus sp. Gilbert, a Marine Bacterium Isolated from the Bottom Sediment of Algoa Bay Nontembiso, Piyo Sekelwa, Cosa Leonard, Mabinya V. Anthony, Okoh I. Mar Drugs Article The bioflocculant-producing potentials of a marine bacteria isolated from the bottom sediment of Algoa Bay was investigated using standard methods. The 16S rDNA sequence analysis revealed 98% similarity to that of Bacillus sp. HXG-C1 and the nucleotide sequence was deposited in GenBank as Bacillus sp. Gilbert with accession number HQ537128. Bioflocculant was optimally produced when sucrose (72% flocculating activity) and ammonium chloride (91% flocculating activity) were used as sole sources of carbon and nitrogen, respectively; an initial pH 6.2 of the production medium; and Mg(2+) as cation. Chemical analysis of the purified bioflocculant revealed the compound to be a polysaccharide. Molecular Diversity Preservation International 2011-07-11 /pmc/articles/PMC3148500/ /pubmed/21822413 http://dx.doi.org/10.3390/md9071232 Text en © 2011 by the authors; licensee MDPI, Basel, Switzerland http://creativecommons.org/licenses/by/3.0 This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Nontembiso, Piyo
Sekelwa, Cosa
Leonard, Mabinya V.
Anthony, Okoh I.
Assessment of Bioflocculant Production by Bacillus sp. Gilbert, a Marine Bacterium Isolated from the Bottom Sediment of Algoa Bay
title Assessment of Bioflocculant Production by Bacillus sp. Gilbert, a Marine Bacterium Isolated from the Bottom Sediment of Algoa Bay
title_full Assessment of Bioflocculant Production by Bacillus sp. Gilbert, a Marine Bacterium Isolated from the Bottom Sediment of Algoa Bay
title_fullStr Assessment of Bioflocculant Production by Bacillus sp. Gilbert, a Marine Bacterium Isolated from the Bottom Sediment of Algoa Bay
title_full_unstemmed Assessment of Bioflocculant Production by Bacillus sp. Gilbert, a Marine Bacterium Isolated from the Bottom Sediment of Algoa Bay
title_short Assessment of Bioflocculant Production by Bacillus sp. Gilbert, a Marine Bacterium Isolated from the Bottom Sediment of Algoa Bay
title_sort assessment of bioflocculant production by bacillus sp. gilbert, a marine bacterium isolated from the bottom sediment of algoa bay
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3148500/
https://www.ncbi.nlm.nih.gov/pubmed/21822413
http://dx.doi.org/10.3390/md9071232
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