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Transplantation of embryonic spleen tissue reveals a role for adult non-lymphoid cells in initiating lymphoid tissue organization

In this report we describe a transplantation system where embryonic spleens are grafted into adult hosts. This model can be used to analyze the cellular and molecular requirements for the development and organization of splenic microenvironments. Whole embryonic day 15 (ED15) spleens, grafted under...

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Autores principales: Glanville, Stephanie H, Bekiaris, Vasileios, Jenkinson, Eric J, Lane, Peter J L, Anderson, Graham, Withers, David R
Formato: Online Artículo Texto
Lenguaje:English
Publicado: WILEY-VCH Verlag 2009
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3149129/
https://www.ncbi.nlm.nih.gov/pubmed/19089813
http://dx.doi.org/10.1002/eji.200838724
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author Glanville, Stephanie H
Bekiaris, Vasileios
Jenkinson, Eric J
Lane, Peter J L
Anderson, Graham
Withers, David R
author_facet Glanville, Stephanie H
Bekiaris, Vasileios
Jenkinson, Eric J
Lane, Peter J L
Anderson, Graham
Withers, David R
author_sort Glanville, Stephanie H
collection PubMed
description In this report we describe a transplantation system where embryonic spleens are grafted into adult hosts. This model can be used to analyze the cellular and molecular requirements for the development and organization of splenic microenvironments. Whole embryonic day 15 (ED15) spleens, grafted under the kidney capsule of adult mice, were colonized by host-derived lymphocytes and DC and developed normal splenic architecture. Grafts were also able to form germinal centers in response to T-dependent antigen. Using this system we demonstrated that adult host-derived lymphotoxin (LT) α was sufficient for the development of ED15 LTα(−/−) grafts. Grafting of ED15 LTα(−/−) spleens into RAG(−/−) hosts followed by transfer of LT α(−/−) splenocytes revealed no requirement for lymphocyte-derived LT α in the induction of CCL21 or the development of T-zone stroma. These data suggest that interactions between adult lymphoid-tissue inducer-like cells and embryonic stromal cells initiated T-zone development. Furthermore, adult lymphoid tissue inducer-like cells were shown to develop from bone marrow-derived progenitors. The model described here demonstrates a method of transferring whole splenic microenvironments and dissecting the stromal and hematopoietic signals involved in spleen development and organization.
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spelling pubmed-31491292011-08-03 Transplantation of embryonic spleen tissue reveals a role for adult non-lymphoid cells in initiating lymphoid tissue organization Glanville, Stephanie H Bekiaris, Vasileios Jenkinson, Eric J Lane, Peter J L Anderson, Graham Withers, David R Eur J Immunol Leukocyte Signaling In this report we describe a transplantation system where embryonic spleens are grafted into adult hosts. This model can be used to analyze the cellular and molecular requirements for the development and organization of splenic microenvironments. Whole embryonic day 15 (ED15) spleens, grafted under the kidney capsule of adult mice, were colonized by host-derived lymphocytes and DC and developed normal splenic architecture. Grafts were also able to form germinal centers in response to T-dependent antigen. Using this system we demonstrated that adult host-derived lymphotoxin (LT) α was sufficient for the development of ED15 LTα(−/−) grafts. Grafting of ED15 LTα(−/−) spleens into RAG(−/−) hosts followed by transfer of LT α(−/−) splenocytes revealed no requirement for lymphocyte-derived LT α in the induction of CCL21 or the development of T-zone stroma. These data suggest that interactions between adult lymphoid-tissue inducer-like cells and embryonic stromal cells initiated T-zone development. Furthermore, adult lymphoid tissue inducer-like cells were shown to develop from bone marrow-derived progenitors. The model described here demonstrates a method of transferring whole splenic microenvironments and dissecting the stromal and hematopoietic signals involved in spleen development and organization. WILEY-VCH Verlag 2009-01 /pmc/articles/PMC3149129/ /pubmed/19089813 http://dx.doi.org/10.1002/eji.200838724 Text en Copyright © 2009 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim http://creativecommons.org/licenses/by/2.5/ Re-use of this article is permitted in accordance with the Creative Commons Deed, Attribution 2.5, which does not permit commercial exploitation.
spellingShingle Leukocyte Signaling
Glanville, Stephanie H
Bekiaris, Vasileios
Jenkinson, Eric J
Lane, Peter J L
Anderson, Graham
Withers, David R
Transplantation of embryonic spleen tissue reveals a role for adult non-lymphoid cells in initiating lymphoid tissue organization
title Transplantation of embryonic spleen tissue reveals a role for adult non-lymphoid cells in initiating lymphoid tissue organization
title_full Transplantation of embryonic spleen tissue reveals a role for adult non-lymphoid cells in initiating lymphoid tissue organization
title_fullStr Transplantation of embryonic spleen tissue reveals a role for adult non-lymphoid cells in initiating lymphoid tissue organization
title_full_unstemmed Transplantation of embryonic spleen tissue reveals a role for adult non-lymphoid cells in initiating lymphoid tissue organization
title_short Transplantation of embryonic spleen tissue reveals a role for adult non-lymphoid cells in initiating lymphoid tissue organization
title_sort transplantation of embryonic spleen tissue reveals a role for adult non-lymphoid cells in initiating lymphoid tissue organization
topic Leukocyte Signaling
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3149129/
https://www.ncbi.nlm.nih.gov/pubmed/19089813
http://dx.doi.org/10.1002/eji.200838724
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