Cargando…
Chemoselective imaging of mouse brain tissue via multiplex CARS microscopy
The fast and reliable characterization of pathological tissue is a debated topic in the application of vibrational spectroscopy in medicine. In the present work we apply multiplex coherent anti-Stokes Raman scattering (MCARS) to the investigation of fresh mouse brain tissue. The combination of imagi...
Autores principales: | , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Optical Society of America
2011
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3149512/ https://www.ncbi.nlm.nih.gov/pubmed/21833351 http://dx.doi.org/10.1364/BOE.2.002110 |
_version_ | 1782209454477934592 |
---|---|
author | Pohling, Christoph Buckup, Tiago Pagenstecher, Axel Motzkus, Marcus |
author_facet | Pohling, Christoph Buckup, Tiago Pagenstecher, Axel Motzkus, Marcus |
author_sort | Pohling, Christoph |
collection | PubMed |
description | The fast and reliable characterization of pathological tissue is a debated topic in the application of vibrational spectroscopy in medicine. In the present work we apply multiplex coherent anti-Stokes Raman scattering (MCARS) to the investigation of fresh mouse brain tissue. The combination of imaginary part extraction followed by principal component analysis led to color contrast between grey and white matter as well as layers of granule and Purkinje cells. Additional quantitative information was obtained by using a decomposition algorithm. The results perfectly agree with HE stained references slides prepared separately making multiplex CARS an ideal approach for chemoselective imaging. |
format | Online Article Text |
id | pubmed-3149512 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | Optical Society of America |
record_format | MEDLINE/PubMed |
spelling | pubmed-31495122011-08-10 Chemoselective imaging of mouse brain tissue via multiplex CARS microscopy Pohling, Christoph Buckup, Tiago Pagenstecher, Axel Motzkus, Marcus Biomed Opt Express Microscopy The fast and reliable characterization of pathological tissue is a debated topic in the application of vibrational spectroscopy in medicine. In the present work we apply multiplex coherent anti-Stokes Raman scattering (MCARS) to the investigation of fresh mouse brain tissue. The combination of imaginary part extraction followed by principal component analysis led to color contrast between grey and white matter as well as layers of granule and Purkinje cells. Additional quantitative information was obtained by using a decomposition algorithm. The results perfectly agree with HE stained references slides prepared separately making multiplex CARS an ideal approach for chemoselective imaging. Optical Society of America 2011-06-30 /pmc/articles/PMC3149512/ /pubmed/21833351 http://dx.doi.org/10.1364/BOE.2.002110 Text en ©2011 Optical Society of America http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-No Derivative Works 3.0 Unported License, which permits download and redistribution, provided that the original work is properly cited. This license restricts the article from being modified or used commercially. |
spellingShingle | Microscopy Pohling, Christoph Buckup, Tiago Pagenstecher, Axel Motzkus, Marcus Chemoselective imaging of mouse brain tissue via multiplex CARS microscopy |
title | Chemoselective imaging of mouse brain tissue via multiplex CARS microscopy |
title_full | Chemoselective imaging of mouse brain tissue via multiplex CARS microscopy |
title_fullStr | Chemoselective imaging of mouse brain tissue via multiplex CARS microscopy |
title_full_unstemmed | Chemoselective imaging of mouse brain tissue via multiplex CARS microscopy |
title_short | Chemoselective imaging of mouse brain tissue via multiplex CARS microscopy |
title_sort | chemoselective imaging of mouse brain tissue via multiplex cars microscopy |
topic | Microscopy |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3149512/ https://www.ncbi.nlm.nih.gov/pubmed/21833351 http://dx.doi.org/10.1364/BOE.2.002110 |
work_keys_str_mv | AT pohlingchristoph chemoselectiveimagingofmousebraintissueviamultiplexcarsmicroscopy AT buckuptiago chemoselectiveimagingofmousebraintissueviamultiplexcarsmicroscopy AT pagenstecheraxel chemoselectiveimagingofmousebraintissueviamultiplexcarsmicroscopy AT motzkusmarcus chemoselectiveimagingofmousebraintissueviamultiplexcarsmicroscopy |