A Gene Optimization Strategy that Enhances Production of Fully Functional P-Glycoprotein in Pichia pastoris

BACKGROUND: Structural and biochemical studies of mammalian membrane proteins remain hampered by inefficient production of pure protein. We explored codon optimization based on highly expressed Pichia pastoris genes to enhance co-translational folding and production of P-glycoprotein (Pgp), an ATP-d...

Descripción completa

Detalles Bibliográficos
Autores principales: Bai, Jiangping, Swartz, Douglas J., Protasevich, Irina I., Brouillette, Christie G., Harrell, Patina M., Hildebrandt, Ellen, Gasser, Brigitte, Mattanovich, Diethard, Ward, Andrew, Chang, Geoffrey, Urbatsch, Ina L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2011
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3149604/
https://www.ncbi.nlm.nih.gov/pubmed/21826197
http://dx.doi.org/10.1371/journal.pone.0022577

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3149604/
https://www.ncbi.nlm.nih.gov/pubmed/21826197
http://dx.doi.org/10.1371/journal.pone.0022577

Ejemplares similares