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A Novel Acyl-CoA Beta-Transaminase Characterized from a Metagenome

BACKGROUND: Bacteria are key components in all ecosystems. However, our knowledge of bacterial metabolism is based solely on the study of cultivated organisms which represent just a tiny fraction of microbial diversity. To access new enzymatic reactions and new or alternative pathways, we investigat...

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Autores principales: Perret, Alain, Lechaplais, Christophe, Tricot, Sabine, Perchat, Nadia, Vergne, Carine, Pellé, Christine, Bastard, Karine, Kreimeyer, Annett, Vallenet, David, Zaparucha, Anne, Weissenbach, Jean, Salanoubat, Marcel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3149608/
https://www.ncbi.nlm.nih.gov/pubmed/21826218
http://dx.doi.org/10.1371/journal.pone.0022918
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author Perret, Alain
Lechaplais, Christophe
Tricot, Sabine
Perchat, Nadia
Vergne, Carine
Pellé, Christine
Bastard, Karine
Kreimeyer, Annett
Vallenet, David
Zaparucha, Anne
Weissenbach, Jean
Salanoubat, Marcel
author_facet Perret, Alain
Lechaplais, Christophe
Tricot, Sabine
Perchat, Nadia
Vergne, Carine
Pellé, Christine
Bastard, Karine
Kreimeyer, Annett
Vallenet, David
Zaparucha, Anne
Weissenbach, Jean
Salanoubat, Marcel
author_sort Perret, Alain
collection PubMed
description BACKGROUND: Bacteria are key components in all ecosystems. However, our knowledge of bacterial metabolism is based solely on the study of cultivated organisms which represent just a tiny fraction of microbial diversity. To access new enzymatic reactions and new or alternative pathways, we investigated bacterial metabolism through analyses of uncultivated bacterial consortia. METHODOLOGY/PRINCIPAL FINDINGS: We applied the gene context approach to assembled sequences of the metagenome of the anaerobic digester of a municipal wastewater treatment plant, and identified a new gene which may participate in an alternative pathway of lysine fermentation. CONCLUSIONS: We characterized a novel, unique aminotransferase that acts exclusively on Coenzyme A (CoA) esters, and proposed a variant route for lysine fermentation. Results suggest that most of the lysine fermenting organisms use this new pathway in the digester. Its presence in organisms representative of two distinct bacterial divisions indicate that it may also be present in other organisms.
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spelling pubmed-31496082011-08-08 A Novel Acyl-CoA Beta-Transaminase Characterized from a Metagenome Perret, Alain Lechaplais, Christophe Tricot, Sabine Perchat, Nadia Vergne, Carine Pellé, Christine Bastard, Karine Kreimeyer, Annett Vallenet, David Zaparucha, Anne Weissenbach, Jean Salanoubat, Marcel PLoS One Research Article BACKGROUND: Bacteria are key components in all ecosystems. However, our knowledge of bacterial metabolism is based solely on the study of cultivated organisms which represent just a tiny fraction of microbial diversity. To access new enzymatic reactions and new or alternative pathways, we investigated bacterial metabolism through analyses of uncultivated bacterial consortia. METHODOLOGY/PRINCIPAL FINDINGS: We applied the gene context approach to assembled sequences of the metagenome of the anaerobic digester of a municipal wastewater treatment plant, and identified a new gene which may participate in an alternative pathway of lysine fermentation. CONCLUSIONS: We characterized a novel, unique aminotransferase that acts exclusively on Coenzyme A (CoA) esters, and proposed a variant route for lysine fermentation. Results suggest that most of the lysine fermenting organisms use this new pathway in the digester. Its presence in organisms representative of two distinct bacterial divisions indicate that it may also be present in other organisms. Public Library of Science 2011-08-03 /pmc/articles/PMC3149608/ /pubmed/21826218 http://dx.doi.org/10.1371/journal.pone.0022918 Text en Perret et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Perret, Alain
Lechaplais, Christophe
Tricot, Sabine
Perchat, Nadia
Vergne, Carine
Pellé, Christine
Bastard, Karine
Kreimeyer, Annett
Vallenet, David
Zaparucha, Anne
Weissenbach, Jean
Salanoubat, Marcel
A Novel Acyl-CoA Beta-Transaminase Characterized from a Metagenome
title A Novel Acyl-CoA Beta-Transaminase Characterized from a Metagenome
title_full A Novel Acyl-CoA Beta-Transaminase Characterized from a Metagenome
title_fullStr A Novel Acyl-CoA Beta-Transaminase Characterized from a Metagenome
title_full_unstemmed A Novel Acyl-CoA Beta-Transaminase Characterized from a Metagenome
title_short A Novel Acyl-CoA Beta-Transaminase Characterized from a Metagenome
title_sort novel acyl-coa beta-transaminase characterized from a metagenome
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3149608/
https://www.ncbi.nlm.nih.gov/pubmed/21826218
http://dx.doi.org/10.1371/journal.pone.0022918
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