Selective Removal of Promoter Nucleosomes by the RSC Chromatin Remodeling Complex

Purified chromatin rings, excised from the PHO5 locus of yeast in transcriptionally repressed and activated states, were remodeled with RSC and ATP. Nucleosomes were translocated, and those originating on the promoter of repressed rings were removed, whereas those originating on the open reading fra...

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Detalles Bibliográficos
Autores principales: Lorch, Yahli, Griesenbeck, Joachim, Boeger, Hinrich, Maier-Davis, Barbara, Kornberg, Roger D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2011
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3150231/
https://www.ncbi.nlm.nih.gov/pubmed/21725295
http://dx.doi.org/10.1038/nsmb.2072
Descripción
Sumario:Purified chromatin rings, excised from the PHO5 locus of yeast in transcriptionally repressed and activated states, were remodeled with RSC and ATP. Nucleosomes were translocated, and those originating on the promoter of repressed rings were removed, whereas those originating on the open reading frame (ORF) were retained. Treatment of the repressed rings with histone deacetylase diminished the removal of promoter nucleosomes. These findings point to a principle of promoter chromatin remodeling for transcription, that promoter-specificity resides primarily in the nucleosomes, rather than in the remodeling complex that acts upon them.

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