Selective Removal of Promoter Nucleosomes by the RSC Chromatin Remodeling Complex

Purified chromatin rings, excised from the PHO5 locus of yeast in transcriptionally repressed and activated states, were remodeled with RSC and ATP. Nucleosomes were translocated, and those originating on the promoter of repressed rings were removed, whereas those originating on the open reading fra...

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Autores principales: Lorch, Yahli, Griesenbeck, Joachim, Boeger, Hinrich, Maier-Davis, Barbara, Kornberg, Roger D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2011
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3150231/
https://www.ncbi.nlm.nih.gov/pubmed/21725295
http://dx.doi.org/10.1038/nsmb.2072
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author Lorch, Yahli
Griesenbeck, Joachim
Boeger, Hinrich
Maier-Davis, Barbara
Kornberg, Roger D.
author_facet Lorch, Yahli
Griesenbeck, Joachim
Boeger, Hinrich
Maier-Davis, Barbara
Kornberg, Roger D.
author_sort Lorch, Yahli
collection PubMed
description Purified chromatin rings, excised from the PHO5 locus of yeast in transcriptionally repressed and activated states, were remodeled with RSC and ATP. Nucleosomes were translocated, and those originating on the promoter of repressed rings were removed, whereas those originating on the open reading frame (ORF) were retained. Treatment of the repressed rings with histone deacetylase diminished the removal of promoter nucleosomes. These findings point to a principle of promoter chromatin remodeling for transcription, that promoter-specificity resides primarily in the nucleosomes, rather than in the remodeling complex that acts upon them.
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spelling pubmed-31502312012-02-01 Selective Removal of Promoter Nucleosomes by the RSC Chromatin Remodeling Complex Lorch, Yahli Griesenbeck, Joachim Boeger, Hinrich Maier-Davis, Barbara Kornberg, Roger D. Nat Struct Mol Biol Article Purified chromatin rings, excised from the PHO5 locus of yeast in transcriptionally repressed and activated states, were remodeled with RSC and ATP. Nucleosomes were translocated, and those originating on the promoter of repressed rings were removed, whereas those originating on the open reading frame (ORF) were retained. Treatment of the repressed rings with histone deacetylase diminished the removal of promoter nucleosomes. These findings point to a principle of promoter chromatin remodeling for transcription, that promoter-specificity resides primarily in the nucleosomes, rather than in the remodeling complex that acts upon them. 2011-07-03 /pmc/articles/PMC3150231/ /pubmed/21725295 http://dx.doi.org/10.1038/nsmb.2072 Text en Users may view, print, copy, download and text and data- mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use: http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Lorch, Yahli
Griesenbeck, Joachim
Boeger, Hinrich
Maier-Davis, Barbara
Kornberg, Roger D.
Selective Removal of Promoter Nucleosomes by the RSC Chromatin Remodeling Complex
title Selective Removal of Promoter Nucleosomes by the RSC Chromatin Remodeling Complex
title_full Selective Removal of Promoter Nucleosomes by the RSC Chromatin Remodeling Complex
title_fullStr Selective Removal of Promoter Nucleosomes by the RSC Chromatin Remodeling Complex
title_full_unstemmed Selective Removal of Promoter Nucleosomes by the RSC Chromatin Remodeling Complex
title_short Selective Removal of Promoter Nucleosomes by the RSC Chromatin Remodeling Complex
title_sort selective removal of promoter nucleosomes by the rsc chromatin remodeling complex
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3150231/
https://www.ncbi.nlm.nih.gov/pubmed/21725295
http://dx.doi.org/10.1038/nsmb.2072
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